scholarly journals MicroRNA expression profile in Lampetra morii upon Vibrio anguillarum infection and miR-4561 characterization targeting lip

2021 ◽  
Vol 4 (1) ◽  
Author(s):  
Lie Ma ◽  
Meng Gou ◽  
Zeyu Du ◽  
Ting Zhu ◽  
Jun Li ◽  
...  
Keyword(s):  
Immune Regulation ◽  
Genetic Information ◽  
Vibrio Anguillarum ◽  
Microrna Expression ◽  
Seed Region ◽  
Bacterial Clearance ◽  
Embryonic Cells ◽  
Immunological Stress ◽  
Expression Atlas ◽  
Induced Apoptosis

AbstractAs a critical evolutionary pivot between invertebrates and vertebrates, lampreys provide rich genetic information. Lamprey immune protein (LIP) is a key immune regulator. MicroRNAs, well-conserved in the response to immunological stress, remain understudied in lamprey immunity. We generated a lamprey microRNA expression atlas, using deep sequencing, upon Vibrio anguillarum infection. Using comparative methods, we found that miR-4561 potentially regulates innate immunity via interaction with lip. We found a sequence in the 3′-UTR region of LIP mRNA complementary to the miR-4561 seed region; miR-4561 expression was negatively correlated with LIP. During V. anguillarum infection, miR-4561 inhibited LIP expression and bacterial clearance. Notably, LIP expression in supraneural body cells was necessary for the Gram-negative immune response. Additionally, we observed that overexpression of miR-4561 induced apoptosis in embryonic cells, suggesting a role in embryonic development. Collectively, we show lamprey microRNAs may significantly affect gene regulation and provide new insights on LIP-mediated immune regulation.

Berberine alleviates LPS-induced apoptosis, oxidation, and skewed lineages during mouse preimplantation development

2022 ◽  
Author(s):  
Xiaosu Miao ◽  
Wei Cui
Keyword(s):  
Inner Cell Mass ◽  
Polycystic Ovary ◽  
Cell Mass ◽  
Cell Lineage ◽  
Culture Conditions ◽  
Preimplantation Development ◽  
Embryonic Cells ◽  
Preimplantation Embryo Development ◽  
New Treatment ◽  
Induced Apoptosis

Abstract Female infertility is a heterogeneous disorder with a variety of complex causes, including inflammation and oxidative stress, which are also closely associated with the pathogenesis of Polycystic Ovary Syndrome (PCOS). As a new treatment for PCOS, berberine (BER), a natural compound from Berberis, has been clinically applied recently. However, the mechanisms underlying the association between BER and embryogenesis are still largely unknown. In this study, effects of BER on preimplantation development was evaluated by using both normal and inflammatory culture conditions induced by lipopolysaccharide (LPS) in the mouse. Our data first suggest that BER itself (25 nM) does not affect embryo quality or future developmental potency, moreover, it can effectively alleviate LPS-induced embryonic damage by mitigating apoptosis via ROS−/caspase-3-dependent pathways and by suppressing pro-inflammatory cytokines via inhibition of NF-κB signaling pathway during preimplantation embryo development. In addition, skewed cell lineage specification in inner cell mass (ICM) and primitive endoderm (PE) caused by LPS can also be successfully rescued with BER. In summary, these findings for the first time demonstrate the non-toxicity of low doses of BER and its anti-apoptotic and anti-oxidative properties on embryonic cells during mammalian preimplantation development.

scholarly journals miRVine: a microRNA expression atlas of grapevine based on small RNA sequencing

BMC Genomics ◽  
2015 ◽  
Vol 16 (1) ◽  
Author(s):  
Jayakumar Belli Kullan ◽  
Daniela Lopes Paim Pinto ◽  
Edoardo Bertolini ◽  
Marianna Fasoli ◽  
Sara Zenoni ◽  
...  
Keyword(s):  
Rna Sequencing ◽  
Small Rna ◽  
Microrna Expression ◽  
Small Rna Sequencing ◽  
Expression Atlas

scholarly journals A STUDY OF THE MECHANISMS OF DNA AND THYMINE DEGRADATION IN CULTURED HUMAN CELLS INFECTED WITH A LIPOVIRUS

1962 ◽  
Vol 115 (5) ◽  
pp. 967-976 ◽  
Author(s):  
R. Shihman Chang ◽  
H. Liepins
Keyword(s):  
Genetic Information ◽  
Actinomycin D ◽  
Total Radioactivity ◽  
Encephalomyocarditis Virus ◽  
Host Cells ◽  
Embryonic Cells ◽  
Similar Increase ◽  
Physical Chemical ◽  
Cultured Human Cells ◽  
Detectable Quantity

DNA and thymine degradation on cultured human, mouse, and chick cells were studied. Significant increase in DNA-degrading activity was demonstrated in human embryonic cells killed by freeze-thawing, liver cells killed with mitomycin C, mouse embryonic cells infected with encephalomyocarditis virus, and in all cells killed by the lipovirus. Twelve other viral agents, actinomycin D, and 5-fluorodeoxyuridine failed to produce a similar increase. Thymidine-2-C14-labeled cultures, either live, killed, or infected by 19 different physical-chemical and biological agents, did not release detectable quantity of C14C2. Following infection with the lipovirus 20 to 60 per cent of the total radioactivity of thymidine-2-C14-labeled cultures was liberated as C14O2. It was postulated that the lipovirus introduced into the host cells the missing genetic information necessary for the synthesis of one or more enzymes responsible for the reductive catabolism of thymine.

scholarly journals A Mammalian microRNA Expression Atlas Based on Small RNA Library Sequencing

Cell ◽  
2007 ◽  
Vol 129 (7) ◽  
pp. 1401-1414 ◽  
Author(s):  
Pablo Landgraf ◽  
Mirabela Rusu ◽  
Robert Sheridan ◽  
Alain Sewer ◽  
Nicola Iovino ◽  
...  
Keyword(s):  
Small Rna ◽  
Microrna Expression ◽  
Expression Atlas ◽  
Small Rna Library

scholarly journals Blockade of ICAM-1 Improves the Outcome of Polymicrobial Sepsis via Modulating Neutrophil Migration and Reversing Immunosuppression

2014 ◽  
Vol 2014 ◽  
pp. 1-10 ◽  
Author(s):  
Yan-jun Zhao ◽  
Wen-jing Yi ◽  
Xiao-jian Wan ◽  
Jun Wang ◽  
Tian-zhu Tao ◽  
...  
Keyword(s):  
Lung Injury ◽  
Adhesion Molecule ◽  
Neutrophil Migration ◽  
Cecal Ligation ◽  
Costimulatory Molecules ◽  
Polymicrobial Sepsis ◽  
Intercellular Adhesion Molecule ◽  
Bacterial Clearance ◽  
Intercellular Adhesion Molecule 1 ◽  
Induced Apoptosis

Intercellular adhesion molecule-1 (ICAM-1) is a key adhesion molecule mediating neutrophil migration and infiltration during sepsis. But its role in the outcome of sepsis remains contradictory. The current study was performed to investigate the role of anti-ICAM-1 antibody in the outcome of polymicrobial sepsis and sepsis-induced immune disturbance. Effect of anti-ICAM-1 antibody on outcome of sepsis induced by cecal ligation and puncture (CLP) was evaluated by the survival analysis, bacterial clearance, and lung injury. Its influence on neutrophil migration and infiltration, as well as lymphocyte status, in thymus and spleen was also investigated. The results demonstrated that ICAM-1 mRNA was upregulated in lung, thymus, and spleen of CLP mice. Anti-ICAM-1 antibody improved survival and bacterial clearance in CLP mice and attenuated lung injury. Migration of neutrophils to peritoneal cavity was enhanced while their infiltration into lung, thymus, and spleen was hampered by ICAM-1 blockade. Anti-ICAM-1 antibody also prevented sepsis-induced apoptosis in thymus and spleen. Positive costimulatory molecules including CD28, CD80, and CD86 were upregulated, while negative costimulatory molecules including PD-1 and PD-L1 were downregulated following anti-ICAM-1 antibody administration. In conclusion, ICAM-1 blockade may improve outcome of sepsis. The rationale may include the modulated neutrophil migration and the reversed immunosuppression.

scholarly journals Gene expression variation in arabidopsis embryos at single-nucleus resolution

Development ◽  
2021 ◽  
Author(s):  
Ping Kao ◽  
Michael A. Schon ◽  
Magdalena Mosiolek ◽  
Balaji Enugutti ◽  
Michael D. Nodine
Keyword(s):  
Gene Expression ◽  
Molecular Mechanisms ◽  
Cell Types ◽  
Embryonic Cell ◽  
Specific Information ◽  
Embryonic Cells ◽  
Basic Body ◽  
Expression Atlas ◽  
Single Nucleus ◽  
Transcriptional Regulatory Mechanisms

Soon after fertilization of egg and sperm, plant genomes become transcriptionally activated and drive a series of coordinated cell divisions to form the basic body plan during embryogenesis. Early embryonic cells rapidly diversify from each other, and investigation of the corresponding gene expression dynamics can help elucidate underlying cellular differentiation programs. However, current plant embryonic transcriptome datasets either lack cell-specific information or have RNA contamination from surrounding non-embryonic tissues. We have coupled fluorescence-activated nuclei sorting together with single-nucleus mRNA sequencing to construct a gene expression atlas of Arabidopsis thaliana early embryos at single-cell resolution. In addition to characterizing cell-specific transcriptomes, we found evidence that distinct epigenetic and transcriptional regulatory mechanisms operate across emerging embryonic cell types. These datasets and analyses, as well as the approach we devised, are expected to facilitate the discovery of molecular mechanisms underlying pattern formation in plant embryos.

scholarly journals MirZ: an integrated microRNA expression atlas and target prediction resource

2009 ◽  
Vol 37 (Web Server) ◽  
pp. W266-W272 ◽  
Author(s):  
J. Hausser ◽  
P. Berninger ◽  
C. Rodak ◽  
Y. Jantscher ◽  
S. Wirth ◽  
...  
Keyword(s):  
Target Prediction ◽  
Microrna Expression ◽  
Expression Atlas

scholarly journals Gene Expression Variation in Arabidopsis Embryos at Single-Nucleus Resolution

2021 ◽  
Author(s):  
Ping Kao ◽  
Michael A Schon ◽  
Magdalena Mosiolek ◽  
Michael D Nodine
Keyword(s):  
Gene Expression ◽  
Molecular Mechanisms ◽  
Cell Types ◽  
Embryonic Cell ◽  
Specific Information ◽  
Embryonic Cells ◽  
Basic Body ◽  
Expression Atlas ◽  
Single Nucleus ◽  
Transcriptional Regulatory Mechanisms

Soon after fertilization of egg and sperm, plant genomes become transcriptionally activated and drive a series of coordinated cell divisions to form the basic body plan during embryogenesis. Early embryonic cells rapidly diversify from each other, and investigation of the corresponding gene expression dynamics can help elucidate underlying cellular differentiation programs. However, current plant embryonic transcriptome datasets either lack cell-specific information or have RNA contamination from surrounding non-embryonic tissues. We have coupled fluorescence-activated nuclei sorting together with single-nucleus mRNA sequencing to construct a gene expression atlas of Arabidopsis thaliana early embryos at single-cell resolution. In addition to characterizing cell-specific transcriptomes, we found evidence that distinct epigenetic and transcriptional regulatory mechanisms operate across emerging embryonic cell types. These datasets and analyses, as well as the approach we devised, are expected to facilitate the discovery of molecular mechanisms underlying pattern formation in plant embryos.

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