scholarly journals Mitigating the non-specific uptake of immunomagnetic microparticles enables the extraction of endothelium from human fat

2021 ◽  
Vol 4 (1) ◽  
Author(s):  
Jeremy A. Antonyshyn ◽  
Vienna Mazzoli ◽  
Meghan J. McFadden ◽  
Anthony O. Gramolini ◽  
Stefan O. P. Hofer ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Endothelial Cells ◽  
Adipose Tissue ◽  
Stromal Cells ◽  
Reliable Method ◽  
Vascular Tissue ◽  
Human Adipose Tissue ◽  
Vascular Tissue Engineering ◽  
Microvascular Endothelial Cells ◽  
Specific Uptake

AbstractEndothelial cells are among the fundamental building blocks for vascular tissue engineering. However, a clinically viable source of endothelium has continued to elude the field. Here, we demonstrate the feasibility of sourcing autologous endothelium from human fat – an abundant and uniquely dispensable tissue that can be readily harvested with minimally invasive procedures. We investigate the challenges underlying the overgrowth of human adipose tissue-derived microvascular endothelial cells by stromal cells to facilitate the development of a reliable method for their acquisition. Magnet-assisted cell sorting strategies are established to mitigate the non-specific uptake of immunomagnetic microparticles, enabling the enrichment of endothelial cells to purities that prevent their overgrowth by stromal cells. This work delineates a reliable method for acquiring human adipose tissue-derived microvascular endothelial cells in large quantities with high purities that can be readily applied in future vascular tissue engineering applications.

Endothelial cells (ECs) for vascular tissue engineering: venous ECs are less thrombogenic than arterial ECs

2012 ◽  
Vol 9 (5) ◽  
pp. 564-576 ◽  
Author(s):  
I. L. A. Geenen ◽  
D. G. M. Molin ◽  
N. M. S. van den Akker ◽  
F. Jeukens ◽  
H. M. Spronk ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Endothelial Cells ◽  
Vascular Tissue ◽  
Vascular Tissue Engineering

Identification and Reduction of Cryoinjury in Endothelial Cells: A First Step toward Establishing a Cell Bank for Vascular Tissue Engineering

2006 ◽  
Vol 0 (0) ◽  
pp. 061114080139001
Author(s):  
Karla Lehle ◽  
Markus Hoenicka ◽  
Volker R. Jacobs ◽  
Franz X. Schmid ◽  
Dietrich E. Birnbaum
Keyword(s):  
Tissue Engineering ◽  
Endothelial Cells ◽  
Vascular Tissue ◽  
Vascular Tissue Engineering ◽  
Cell Bank ◽  
A Cell

Use of Polyelectrolyte Films in Vascular Tissue Engineering

2008 ◽  
Author(s):  
H. Kerdjoudj ◽  
V. Moby ◽  
N. Berthelemy ◽  
J. C. Voegel ◽  
P. Menu ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Endothelial Cells ◽  
Vascular Tissue ◽  
Vascular Endothelial Cells ◽  
Medical Science ◽  
Vascular Diseases ◽  
Vascular Tissue Engineering ◽  
High Morbidity ◽  
Layer By Layer

Vascular diseases with their high morbidity and mortality are a major challenge for medical science, engaging the best minds in modern medicine. The development of antithrombogenic surfaces still remains a huge challenge in the vascular tissue engineering field. Various researchers have expanded surface coating procedures allowing endothelial cells (EC) adhesion and retention on vascular substitutes or by incorporating some of the mechanisms employed by vascular endothelial cells i.e. heparin. The short in vivo patency of these grafts is related. Our group study evaluates a new surface modification based on polyelectrolyte building. The layer by layer self assembly and the result in polyelectrolyte multilayer films (PEM) became also in a recent past a challenging, simple and versatile way to engineer surfaces with highly specific properties. Previous studies indicated that the poly(sodium-4 styrene sulfonate)/poly (allylamine hydrochloride) PSS/PAH multilayered films when ended by PAH induce strong adhesion and retention of mature EC which spread and keep their phenotype as well on glass [1,2], on expanded polytetrafluoroethylene ePTFE [3] and on cryopreserved arteries [4,5]. The mechanical properties (compliance), leading to early intimal hyperplasia and graft failure, were lost after artery cryopreservation. We have demonstrated the compliance restoration of PEM treated cryopreserved close to native arteries [5]. The use of an autologous EC source avoids the immunological rejections of the grafts. With an autologous origin, high proliferation capacity and potentialities to proliferate and differentiate into matures EC, the endothelial progenitor cells (EPC) have raised huge interest and offer new opportunities in vascular engineering. Currents protocols for isolation and differentiation of EPC from peripheral blood requires at least 1 month to observe an endothelium-like morphology and about 2 months for confluent EC monolayer. The EPC cultivated on PEM treated glasses showed a monolayer development after only 14 days of culture. The morphological appearance and mature phenotype markers expression and repartition of the monolayer cells are close to mature EC [6]. These main results have led to French patent deposit in June 2007[7].

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