scholarly journals Comprehensive multiomic characterization of human papillomavirus-driven recurrent respiratory papillomatosis reveals distinct molecular subtypes

2021 ◽  
Vol 4 (1) ◽  
Author(s):  
Cem Sievers ◽  
Yvette Robbins ◽  
Ke Bai ◽  
Xinping Yang ◽  
Paul E. Clavijo ◽  
...  
Keyword(s):  
Human Papillomavirus ◽  
Squamous Cell ◽  
Cellular Proliferation ◽  
Recurrent Respiratory Papillomatosis ◽  
Low Risk ◽  
Viral Immunity ◽  
Upper Aerodigestive Tract ◽  
High Risk Hpv ◽  
Insight Into

AbstractRecurrent respiratory papillomatosis (RRP) is a debilitating neoplastic disorder of the upper aerodigestive tract caused by chronic infection with low-risk human papillomavirus types 6 or 11. Patients with severe RRP can require hundreds of lifetime surgeries to control their disease and pulmonary papillomatosis can be fatal. Here we report the comprehensive genomic and transcriptomic characterization of respiratory papillomas. We discovered and characterized distinct subtypes with transcriptional resemblance to either a basal or differentiated cell state that associate with disease aggressiveness and differ in key molecular, immune and APOBEC mutagenesis profiles. Through integrated comparison with high-risk HPV-associated head and neck squamous cell carcinoma, our analysis revealed divergent molecular and immune papilloma subtypes that form independent of underlying genomic alterations. Cumulatively our results support the development of dysregulated cellular proliferation and suppressed anti-viral immunity through distinct programs of squamous cell differentiation and associated expression of low-risk HPV genes. These analyses provide insight into the pathogenesis of respiratory papillomas and provide a foundation for the development of therapeutic strategies.

scholarly journals Oral squamous cell carcinoma versus oral verrucous carcinoma: an approach to cellular proliferation and negative relation to human papillomavirus (HPV)

Tumor Biology ◽  
2010 ◽  
Vol 32 (2) ◽  
pp. 409-416 ◽  
Author(s):  
José Vieira de Spíndula-Filho ◽  
Aparecido Divino da Cruz ◽  
Angélica Ferreira Oton-Leite ◽  
Aline Carvalho Batista ◽  
Cláudio Rodrigues Leles ◽  
...  
Keyword(s):  
Squamous Cell Carcinoma ◽  
Human Papillomavirus ◽  
Oral Squamous Cell Carcinoma ◽  
Cell Carcinoma ◽  
Squamous Cell ◽  
Cellular Proliferation ◽  
Verrucous Carcinoma ◽  
Negative Relation

scholarly journals Genomic characterization of human papillomavirus-positive and -negative human squamous cell cancer cell lines

Oncotarget ◽  
2017 ◽  
Vol 8 (49) ◽  
pp. 86369-86383 ◽  
Author(s):  
Nene N. Kalu ◽  
Tuhina Mazumdar ◽  
Shaohua Peng ◽  
Li Shen ◽  
Vaishnavi Sambandam ◽  
...  
Keyword(s):  
Human Papillomavirus ◽  
Cell Lines ◽  
Cancer Cell ◽  
Squamous Cell ◽  
Squamous Cell Cancer ◽  
Cell Cancer ◽  
Cancer Cell Lines ◽  
Genomic Characterization

scholarly journals Detection of human papillomavirus oncoprotein E7 in liquid-based cytology

2012 ◽  
Vol 93 (2) ◽  
pp. 356-363 ◽  
Author(s):  
Maria Lidqvist ◽  
Olle Nilsson ◽  
Jan Holmgren ◽  
Sebastian Hölters ◽  
Eva Röijer ◽  
...  
Keyword(s):  
Human Papillomavirus ◽  
High Risk ◽  
Low Risk ◽  
Transformed Cells ◽  
Specific Staining ◽  
Hpv E7 ◽  
Detection And Identification ◽  
Liquid Based Cytology ◽  
E7 Proteins ◽  
High Risk Hpv

The selection and characterization of a set of mouse mAbs against high-risk human papillomavirus (HPV) E7 oncoprotein and the development of protocols for immunocytochemistry (ICC) are described here. A large number of antibodies raised towards HPV16 and 18 E7 were tested for high-risk specificity by ELISA using a panel of HPV E7 proteins. Antibodies detecting low-risk E7 were discarded, resulting in 38 high-risk HPV E7-specific antibodies. The corresponding epitopes were mapped using overlapping HPV E7 fragments displayed on phage particles. Functionality in ICC against formalin-fixed cervical cancer cell lines was demonstrated for ten mAbs; their high-risk specificity was confirmed by Western blot analysis and ICC on transiently transformed cells expressing high- or low-risk HPV E7. These mAbs were specific for one or several of the high-risk strains HPV16, 18, 31, 35 and 45. Specific E7 staining of liquid-based cytology (LBC) samples was demonstrated for seven mAbs and optimized protocols were established. The E716-41 and E718-79 mAbs demonstrated particularly strong and specific staining of cells stored in LBC fluid for at least 6 months. It is proposed that the high-risk HPV E7 staining protocols established in this study may have the potential to be included in a complementary test for the detection and identification of malignantly transformed cells, in for example atypical squamous cells of undetermined significance samples.

Weekly cisplatin and radiotherapy for low-risk human papillomavirus positive oropharyngeal squamous cell carcinoma (HPVOPC).

2014 ◽  
Vol 32 (15_suppl) ◽  
pp. e17017-e17017
Author(s):  
Damien Urban ◽  
June Corry ◽  
Benjamin J. Solomon ◽  
Annette May Ling Lim ◽  
Tsien Fua ◽  
...  
Keyword(s):  
Squamous Cell Carcinoma ◽  
Human Papillomavirus ◽  
Cell Carcinoma ◽  
Squamous Cell ◽  
Oropharyngeal Squamous Cell Carcinoma ◽  
Low Risk ◽  
Weekly Cisplatin

scholarly journals Human papillomavirus in high- and low-risk areas of oesophageal squamous cell carcinoma in China

2007 ◽  
Vol 96 (10) ◽  
pp. 1554-1559 ◽  
Author(s):  
K Shuyama ◽  
A Castillo ◽  
F Aguayo ◽  
Q Sun ◽  
N Khan ◽  
...  
Keyword(s):  
Squamous Cell Carcinoma ◽  
Human Papillomavirus ◽  
Cell Carcinoma ◽  
Squamous Cell ◽  
Low Risk ◽  
Oesophageal Squamous Cell Carcinoma ◽  
Risk Areas

scholarly journals Additional Human Papillomavirus Types Detected by the Hybrid Capture Tube Test among Samples from Women with Cytological and Colposcopical Atypia

2000 ◽  
Vol 38 (1) ◽  
pp. 408-411
Author(s):  
József Kónya ◽  
György Veress ◽  
Attila Juhász ◽  
Krisztina Szarka ◽  
Tamás Sápy ◽  
...  
Keyword(s):  
Human Papillomavirus ◽  
High Risk ◽  
Fragment Length Polymorphism ◽  
Low Risk ◽  
Double Positive ◽  
Detection Range ◽  
Hybrid Capture ◽  
Pcr Rflp ◽  
Hpv Types ◽  
High Risk Hpv

ABSTRACT The type specificity of the human papillomavirus (HPV) Hybrid Capture Tube (HCT) test was evaluated by using typing with PCR (MY09-MY11)-restriction fragment length polymorphism (RFLP) and sequencing. All samples HCT test positive for only low-risk HPV ( n = 15) or only high-risk HPV ( n = 102) were confirmed, whereas 9 of 12 HCT test double-positive samples contained only high-risk HPV types as determined by PCR-RFLP. Several high-risk HPV types (HPV-53, -58, -62, -66, -CP8304, and -MM4) not included in the HCT test were indeed detected, indicating a broader detection range with retained distinction between low-risk and high-risk HPV types.

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