Prognostic implications of type and density of tumour-infiltrating lymphocytes in gastric cancer

Abstract Background To explore CT radiomics and morphologic characteristics for predicting programmed cell death ligand 1 on tumour cells (PD-L1) and tumour infiltrating lymphocytes (PD-L1-TILs) status in gastric cancer (GC). Methods From March 2019 to October 2019, 101 patients identified with GCs who underwent surgery at our hospital were enrolled in this study retrospectively. Radiomic features were extracted from regions of interest manually drawn on venous CT images. Besides, 13 morphologic characteristics were evaluated. The signatures based on radiomics and morphologic characteristics were built using multiple classifiers (Support Vector Machine [SVM], Naive Bayes [NB], Decision Trees [DT], and Random Forest [RF]). Receiver operating characteristic (ROC) curve was performed to assess diagnostic efficiency. Results The adjacent adipose tissue (P = 0.009) and numerous radiomic features (all P < 0.05) differed significantly between GCs with different PD-L1 status. Six radiomic features showed significant differences between different PD-L1-TILs status (all P < 0.05). The highest areas under the ROC curves (AUCs) of signatures generated by classifiers were 0.807 (SVM) and 0.729 (NB) for the prediction of PD-L1 and PD-L1-TILs status, respectively. Conclusions It was promising to predict PD-L1 status in GCs noninvasively using CT radiomics combined with morphologic characteristics. It might help to improve clinical decision making with regard to immunotherapy. However, the prediction for PD-L1-TILs needs to be explored further.

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Study Of The Association Of Tumor Deposits With Tumour-infiltrating Lymphocytes And Prognosis In Gastric Cancer Patients

10.21203/rs.3.rs-754314/v1 ◽

2021 ◽

Author(s):

Xinyue Li ◽

Jing Yang

Keyword(s):

Gastric Cancer ◽

Proportional Hazards ◽

Clinical Stage ◽

Disease Free Survival ◽

Clinicopathological Characteristics ◽

Cox Proportional Hazards ◽

Rank Test ◽

Cancer Specific Survival ◽

Tumour Infiltrating Lymphocytes ◽

Infiltrating Lymphocytes

Abstract Background: To investigate the relationship between tumour deposits(TDs) with the clinicopathological characteristics,prognosis of gastric cancer and tumour-infiltrating lymphocytes( TILs）.Methods: The pathological findings of 369 patients with gastric cancer were retrospectively analysed to observe the expression of TDs, and the levels of stromal TILs . The relationships between TDs status, clinicopathological characteristics, and TILs infiltration level were compared using the chi-square test, and rank data were tested using the rank sum test. Kaplan-Meier was used for survival analysis, and the log-rank test was used to determine the differences in survival curves between groups. The prognostic value of TDs was assessed using multivariate Cox proportional hazards regression analysis.Results: TDs were significantly associated with sex, Lymphovascular invasion, Perineural invasion, pathological TNM stage, and clinical stage (all P<0.05). TILs levels were lower in TDs(+) group and higher in TDs(-) group. TDs(+) group had poor Disease-free survival, cancer-specific survival , and overall survival as compared with TDs(-) groups.Conclusions: TDs is negatively correlated with TILs , and TDs+ was an Independent predictors of the prognosis of gastric cancer.

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Compared expression of Tumour Infiltrating Lymphocytes and FOXA1 in matched pairs of brain metastasis and primary breast cancer and prognostic implications – a pilot study

European Journal of Cancer ◽

10.1016/s0959-8049(20)30697-3 ◽

2020 ◽

Vol 138 ◽

pp. S63-S64

Author(s):

A. Thulin ◽

E. Rönnerman ◽

S. De Lara ◽

C. Chamalidou ◽

A. Schoenfeldt ◽

...

Keyword(s):

Breast Cancer ◽

Pilot Study ◽

Brain Metastasis ◽

Primary Breast Cancer ◽

Matched Pairs ◽

Prognostic Implications ◽

Tumour Infiltrating Lymphocytes ◽

Infiltrating Lymphocytes

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Prognostic role of tumour-infiltrating lymphocytes assessed by H&E-stained section in gastric cancer: a systematic review and meta-analysis

BMJ Open ◽

10.1136/bmjopen-2020-044163 ◽

2021 ◽

Vol 11 (1) ◽

pp. e044163

Author(s):

Chunfang Tian ◽

Haiyan Jing ◽

Caixia Wang ◽

Weibo Wang ◽

Yangang Cui ◽

...

Keyword(s):

Gastric Cancer ◽

Systematic Review ◽

Histological Grade ◽

Meta Analysis ◽

Random Effect ◽

Clinicopathological Characteristics ◽

Cochrane Library ◽

Depth Of Invasion ◽

Tumour Infiltrating Lymphocytes ◽

Infiltrating Lymphocytes

ObjectivesSome studies have identified tumour-infiltrating lymphocytes (TILs) in H&E-stained sections of gastric cancer, but the prognostic and clinicopathological significance of this remains unclear. The objective of this study is to evaluate the associations between H&E-based TIL density and prognosis and clinicopathological characteristics of patients with gastric cancer.DesignSystematic review and meta-analysis.Data sourcesCochrane Library, PubMed and Embase databases were searched through 25 February 2020.Eligibility criteriaStudies evaluating the correlations between TILs assessed by H&E-stained sections and prognosis and clinicopathological characteristics of gastric cancer were included.Data extraction and synthesisRelevant data were extracted and risks of bias were assessed independently by two reviewers. HR and relative risk (RR) with 95% CI were pooled by random-effect models to estimate the associations between TIL density and overall survival (OS) and clinicopathological characteristics, respectively.ResultsWe enrolled nine studies including 2835 cases for the present meta-analysis. High TILs were associated with superior OS (HR=0.68, 95% CI 0.52 to 0.87, p=0.003) compared with low TILs. High TILs were significantly associated with lower depth of invasion (T3–T4 vs T1–T2) (RR=0.58, 95% CI 0.50 to 0.66, p<0.001), less lymph node involvement (presence vs absence) (RR=0.68, 95% CI 0.56 to 0.81, p<0.001) and earlier TNM (tumour, node, metastasis) stage (III–IV vs I–II) (RR=0.68, 95% CI 0.55 to 0.83, p<0.001). TIL density was not associated with age, gender, Lauren classification or histological grade. The methodology for evaluating TIL and its cut-off value varied across different studies, which might affect the results of our meta-analysis.ConclusionsOur meta-analysis suggests that H&E-based TIL density is a reliable biomarker to predict the clinical outcomes of patients with gastric cancer. Multicentre, prospective studies are needed to further confirm our findings.PROSPERO registration numberCRD42020169877.

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