scholarly journals Development of a cell system for siRNA screening of pathogen responses in human and mouse macrophages

2015 ◽  
Vol 5 (1) ◽  
Author(s):  
Ning Li ◽  
Jing Sun ◽  
Zachary L. Benet ◽  
Ze Wang ◽  
Souhaila Al-Khodor ◽  
...  
Keyword(s):  
Cell System ◽  
Mouse Macrophages ◽  
A Cell ◽  
Human And Mouse ◽  
Sirna Screening

Proteomic Characterization of Protein-Protein Interactions

2021 ◽  
Vol 4 (1) ◽  
Author(s):  
Veenstra TD ◽  
Keyword(s):  
Protein Interactions ◽  
Disease Diagnosis ◽  
Cell System ◽  
Protein Protein Interactions ◽  
Novel Technologies ◽  
Cell Functions ◽  
Single Protein ◽  
A Cell ◽  
Molecular Components

Identifying all the molecular components within a living cell is the first step into understanding how it functions. To further understand how a cell functions requires identifying the interactions that occur between these components. This fact is especially relevant for proteins. No protein within a human cell functions on its own without interacting with another biomolecule - usually another protein. While Protein-Protein Interactions (PPI) have historically been determined by examining a single protein per study, novel technologies developed over the past couple of decades are enabling high-throughput methods that aim to describe entire protein networks within cells. In this review, some of the technologies that have led to these developments are described along with applications of these techniques. Ultimately the goal of these technologies is to map out the entire circuitry of PPI within human cells to be able to predict the global consequences of perturbations to the cell system. This predictive capability will have major impacts on the future of both disease diagnosis and treatment.

Desialylated LDL uptake in human and mouse macrophages can be mediated by a lectin receptor

1996 ◽  
Vol 121 (1) ◽  
pp. 151-163 ◽  
Author(s):  
Thomas Grewal ◽  
Anna Bartlett ◽  
James W. Burgess ◽  
Nicolle H. Packer ◽  
Keith K. Stanley
Keyword(s):  
Lectin Receptor ◽  
Mouse Macrophages ◽  
Ldl Uptake ◽  
Human And Mouse

scholarly journals HiCRes: a computational method to estimate and predict the resolution of HiC libraries

2020 ◽  
Author(s):  
Claire Marchal ◽  
Nivedita Singh ◽  
Ximena Corso-Díaz ◽  
Anand Swaroop
Keyword(s):  
Expression Patterns ◽  
Three Dimensional ◽  
Computational Method ◽  
Mathematical Concepts ◽  
Regulate Gene Expression ◽  
Chromatin Interactions ◽  
Genome Wide ◽  
A Cell ◽  
Cell Type Specific ◽  
Human And Mouse

AbstractThree-dimensional (3D) conformation of the chromatin is crucial to stringently regulate gene expression patterns and DNA replication in a cell-type specific manner. HiC is a key technique for measuring 3D chromatin interactions genome wide. Estimating and predicting the resolution of a library is an essential step in any HiC experimental design. Here, we present the mathematical concepts to estimate the resolution of a library and predict whether deeper sequencing would enhance the resolution. We have developed HiCRes, a docker pipeline, by applying these concepts to human and mouse HiC libraries.

scholarly journals Overcoming the Challenges for a Mass Manufacturing Machine for the Assembly of PEMFC Stacks

Machines ◽  
2019 ◽  
Vol 7 (4) ◽  
pp. 66 ◽  
Author(s):  
Porstmann ◽  
Wannemacher ◽  
Richter
Keyword(s):  
Fuel Cell ◽  
Economies Of Scale ◽  
Polymer Electrolyte Membrane ◽  
Cell System ◽  
Active Components ◽  
Fuel Cell Stack ◽  
Electrolyte Membrane ◽  
Modular Concept ◽  
A Cell ◽  
Manufacturing Machine

One of the major obstacles standing in the way of a break-through in fuel cell technology is its relatively high costs compared to well established fossil-based technologies. The reasons for these high costs predominantly lie in the use of non-standardized components, complex system components, and non-automated production of fuel cells. This problem can be identified at multiple levels, for example, the electrochemically active components of the fuel cell stack, peripheral components of the fuel cell system, and eventually on the level of stack and system assembly. This article focused on the industrialization of polymer electrolyte membrane fuel cell (PEMFC) stack components and assembly. To achieve this, the first step is the formulation of the requirement specifications for the automated PEMFC stack production. The developed mass manufacturing machine (MMM) enables a reduction of the assembly time of a cell fuel cell stack to 15 minutes. Furthermore the targeted automation level is theoretically capable of producing up to 10,000 fuel cell stacks per year. This will result in a ~50% stack cost reduction through economies of scale and increased automation. The modular concept is scalable to meet increasing future demand which is essential for the market ramp-up and success of this technology.

scholarly journals Crystalline Silica Impairs Efferocytosis Abilities of Human and Mouse Macrophages: Implication for Silica-Associated Systemic Sclerosis

2020 ◽  
Vol 11 ◽  
Author(s):  
Alain Lescoat ◽  
Alice Ballerie ◽  
Marie Lelong ◽  
Yu Augagneur ◽  
Claudie Morzadec ◽  
...  
Keyword(s):  
Systemic Sclerosis ◽  
Crystalline Silica ◽  
Mouse Macrophages ◽  
Human And Mouse

scholarly journals Phenotypic switch of human and mouse macrophages and resultant effects on apoptosis resistance in hepatocytes

2019 ◽  
Vol 25 (3) ◽  
pp. 176-185 ◽  
Author(s):  
Li Bai ◽  
Yu Chen ◽  
Sujun Zheng ◽  
Feng Ren ◽  
Ming Kong ◽  
...  
Keyword(s):  
Caspase 3 ◽  
Apoptosis Resistance ◽  
Phenotypic Switch ◽  
Novel Treatments ◽  
Mouse Macrophages ◽  
Ifn Γ ◽  
Acute Insult ◽  
Human And Mouse

Acute-on-chronic liver failure (ACLF) carries a significant burden on critical care services and health care resources. However, the exact pathogenesis of ACLF remains to be elucidated, and novel treatments are desperately required. In our previous work, we utilized mice subjected to acute insult in the context of hepatic fibrosis to simulate the development of ACLF and documented the favorable hepatoprotection conferred by M2-like macrophages in vivo and in vitro. In the present study, we focused on the phenotypic switch of human and mouse macrophages and assessed the effects of this switch on apoptosis resistance in hepatocytes. For this purpose, human and mouse macrophages were isolated and polarized into M0, M(IFN-γ), M(IFN-γ→IL-4), M(IL-4) or M(IL-4→IFN-γ) subsets. Conditioned media (CM) from these subsets were applied to human and mouse hepatocytes followed by apoptosis induction. Cell apoptosis was evaluated by immunostaining for cleaved caspase-3. As a result, M(IFN-γ) or M(IL-4) macrophages switched their phenotype into M(IFN-γ→IL-4) or M(IL-4→IFN-γ) through reprogramming with IL-4 or IFN-γ, respectively. Importantly, hepatocytes pre-treated with M(IFN-γ→IL-4) CMs exhibited much weaker expression of cleaved caspase-3, compared to those pre-treated with M(IFN-γ) CM, and vice versa. Together, phenotypic switch of macrophages toward M(IL-4) phenotype confers hepatocytes enhanced resistance to apoptosis.

Genetic programs expressed in resting and IL-4 alternatively activated mouse and human macrophages: similarities and differences

Blood ◽  
2013 ◽  
Vol 121 (9) ◽  
pp. e57-e69 ◽  
Author(s):  
Fernando O. Martinez ◽  
Laura Helming ◽  
Ronny Milde ◽  
Audrey Varin ◽  
Barbro N. Melgert ◽  
...  
Keyword(s):  
M2 Macrophages ◽  
Human Macrophages ◽  
Key Points ◽  
Gene And Protein Expression ◽  
Activated State ◽  
Mouse Macrophages ◽  
Similarities And Differences ◽  
Conserved Gene ◽  
Human And Mouse ◽  
Alternatively Activated

Key Points Human and mouse macrophages share partially conserved gene and protein expression programs in the resting or M2 activated state. TGM2 is a novel M2 marker consistently induced in human and mouse M2 macrophages.

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