scholarly journals Characterization of the transcriptomes and cuticular protein gene expression of alate adult, brachypterous neotenic and adultoid reproductives of Reticulitermes labralis

2016 ◽  
Vol 6 (1) ◽  
Author(s):  
Xiaohong Su ◽  
He Liu ◽  
Xiaojuan Yang ◽  
Jiaoling Chen ◽  
Honggui Zhang ◽  
...  
Keyword(s):  
Gene Expression ◽  
Protein Gene ◽  
Cuticular Protein ◽  
Cuticular Protein Gene

Nucleotide sequence of an adult-specific cuticular protein gene from the beetle Tenebrio molitor: effects of 20-hydroxyecdysone on mRNA accumulation

1993 ◽  
Vol 2 (2) ◽  
pp. 81-88 ◽  
Author(s):  
H. Bouhin ◽  
C. Braquart ◽  
J.-P. Charles ◽  
B. Quennedey ◽  
J. Delachambre
Keyword(s):  
Nucleotide Sequence ◽  
Protein Gene ◽  
Tenebrio Molitor ◽  
Cuticular Protein ◽  
Mrna Accumulation ◽  
Cuticular Protein Gene

Cloning and expression profiling of a cuticular protein gene in Daphnia carinata

2014 ◽  
Vol 224 (3) ◽  
pp. 129-135 ◽  
Author(s):  
Ajing Liu ◽  
Mingqing Zhang ◽  
Ling Kong ◽  
Donglei Wu ◽  
Xianlong Weng ◽  
...  
Keyword(s):  
Expression Profiling ◽  
Protein Gene ◽  
Cloning And Expression ◽  
Cuticular Protein ◽  
Daphnia Carinata ◽  
Cuticular Protein Gene

Characterization of antifreeze protein gene expression in summer spruce budworm larvae

2006 ◽  
Vol 36 (3) ◽  
pp. 210-218 ◽  
Author(s):  
Wensheng Qin ◽  
Michael G. Tyshenko ◽  
Daniel Doucet ◽  
Virginia K. Walker
Keyword(s):  
Gene Expression ◽  
Protein Gene ◽  
Spruce Budworm ◽  
Antifreeze Protein

scholarly journals Characterization of the Mouse Islet-Specific Glucose-6-Phosphatase Catalytic Subunit-Related Protein Gene Promoter by In Situ Footprinting: Correlation With Fusion Gene Expression in the Islet-Derived  TC-3 and Hamster Insulinoma Tumor Cell Lines

Diabetes ◽  
2001 ◽  
Vol 50 (3) ◽  
pp. 502-514 ◽  
Author(s):  
L. J. Bischof ◽  
C. C. Martin ◽  
C. A. Svitek ◽  
B. T. Stadelmaier ◽  
L. A. Hornbuckle ◽  
...  
Keyword(s):  
Gene Expression ◽  
Tumor Cell ◽  
Protein Gene ◽  
Fusion Gene ◽  
Gene Promoter ◽  
Tumor Cell Lines ◽  
Related Protein ◽  
Mouse Islet

scholarly journals Comparative transcriptomic analysis and endocuticular protein gene expression of alate adults, workers and soldiers of the termite Reticulitermes aculabialis

BMC Genomics ◽  
2019 ◽  
Vol 20 (1) ◽  
Author(s):  
Humaira Rasheed ◽  
Chenxu Ye ◽  
Yufeng Meng ◽  
Yuehua Ran ◽  
Jing Li ◽  
...  
Keyword(s):  
Gene Expression ◽  
Protein Gene ◽  
Structural Characteristics ◽  
Division Of Labour ◽  
Structural Proteins ◽  
Pcr Analysis ◽  
Insect Cuticle ◽  
Cuticular Protein ◽  
Qrt Pcr ◽  
Comparative Transcriptomic Analysis

Abstract Background The insect cuticle is mainly composed of exocuticle and endocuticle layers that consist of a large number of structural proteins. The thickness and synthesis of the exocuticle depend on different castes that perform various functions in alates, workers and soldiers. However, it is not clear whether the soft endocuticle is involved in the division of labour in termite colonies. To reveal the structural characteristics of the endocuticle in different castes, we investigated the thickness of endocuticle layers in alates, workers and soldiers of the termite Reticulitermes aculabialis, and then we sequenced their transcriptome and detected the endocuticle protein genes. The differential expression levels of the endocuticular protein genes were confirmed in the three castes. Results We found that there was a great difference in the thickness of the endocuticle among the alates, soldiers and workers. The thickest endocuticle layers were found in the heads of the workers 7.88 ± 1.67 μm. The endocuticle layer in the head of the workers was approximately three-fold and nine-fold thicker than that in the heads of soldiers and alates, respectively. The thinnest endocuticle layers occurred in the head, thorax and abdomen of alates, which were 0.86 ± 0.15, 0.76 ± 0.24 and 0.52 ± 0.17 μm thick, respectively, and had no significant differences. A total of 43,531,650 clean sequencing reads was obtained, and 89,475 unigenes were assembled. Of the 70 identified cuticular protein genes, 10 endocuticular genes that belong to the RR-1 family were selected. qRT-PCR analysis of the five endocuticular genes (SgAbd-2, SgAbd-9, Abd-5, SgAbd-2-like and Abd-4-like) revealed that the endocuticle genes were more highly expressed in workers than in soldiers and alates. Conclusion These results suggest that SgAbd and Abd are the key components of the endocuticle. We infer that the thicker endocuticle in workers is helpful for them to perform their functions against environmental stress.

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