An electronic DNA microarray technique for detection and differentiation of viable Campylobacter species

The Analyst ◽  
2006 ◽  
Vol 131 (8) ◽  
pp. 907 ◽  
Author(s):  
Hai Zhang ◽  
Zhilong Gong ◽  
Odell Pui ◽  
Yanming Liu ◽  
Xing-Fang Li

2002 ◽  
Vol 184 (9) ◽  
pp. 2344-2351 ◽  
Author(s):  
Mitsuo Ogura ◽  
Hirotake Yamaguchi ◽  
Kazuo Kobayashi ◽  
Naotake Ogasawara ◽  
Yasutaro Fujita ◽  
...  

ABSTRACT The Bacillus subtilis competence transcription factor ComK is required for establishment of competence for genetic transformation. In an attempt to study the ComK factor further, we explored the genes regulated by ComK using the DNA microarray technique. In addition to the genes known to be dependent on ComK for expression, we found many genes or operons whose ComK dependence was not known previously. Among these genes, we confirmed the ComK dependence of 16 genes by using lacZ fusions, and three genes were partially dependent on ComK. Transformation efficiency was significantly reduced in an smf disruption mutant, although disruption of the other ComK-dependent genes did not result in significant decreases in transformation efficiency. Nucleotide sequences similar to that of the ComK box were found for most of the newly discovered genes regulated by ComK.







2006 ◽  
Vol 31 (9) ◽  
pp. 1888-1899 ◽  
Author(s):  
S Hossein Fatemi ◽  
Teri J Reutiman ◽  
Timothy D Folsom ◽  
Christopher Bell ◽  
Lisa Nos ◽  
...  


2011 ◽  
Vol 6 (4) ◽  
pp. 814-826 ◽  
Author(s):  
Ian PG Marshall ◽  
Dusty RV Berggren ◽  
Mohammad F Azizian ◽  
Luke C Burow ◽  
Lewis Semprini ◽  
...  


2005 ◽  
Vol 77 (4) ◽  
pp. 528-540 ◽  
Author(s):  
Henrik Nordström ◽  
Kerstin I. Falk ◽  
Gunnel Lindegren ◽  
Mehrdad Mouzavi-Jazi ◽  
Annelie Waldén ◽  
...  


2009 ◽  
Vol 52 (3) ◽  
pp. 555-566 ◽  
Author(s):  
Regiane de Fátima Travensolo ◽  
Maria Vitória Cecchette Gottardi Costa ◽  
Lucia Maria Carareto-Alves ◽  
Emanuel Carrilho ◽  
Eliana Gertrudes de Macedo Lemos

DNA Microarray was developed to monitor the expression of many genes from Xylella fastidiosa, allowing the side by-side comparison of two situations in a single experiment. The experiments were performed using X. fastidiosa cells grown in two culture media: BCYE and XDM2. The primers were synthesized, spotted onto glass slides and the array was hybridized against fluorescently labeled cDNAs. The emitted signals were quantified, normalized and the data were statistically analyzed to verify the differentially expressed genes. According to the data, 104 genes were differentially expressed in XDM2 and 30 genes in BCYE media. The present study showed that DNA microarray technique efficiently differentiate the expressed genes under different conditions.





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