Differential photodynamic response of cultured cells to methylene blue and toluidine blue: role of dark redox processes

Tissue from the hood and sarcophagus regions were fixed in 6% glutaraldehyde in 1 M.cacodylate buffer and washed in buffer. Tissue for SEM was partially dried, attached to aluminium targets with silver conducting paint, carbon-gold coated(100-500Å), and examined in a Kent Cambridge Stereoscan S4. Tissue for the light microscope was post fixed in 1% aqueous OsO4, dehydrated in acetone (4°C), embedded in Epon 812 and sectioned at ½u on a Sorvall MT 2 ultramicrotome. Cross and longitudinal sections were cut and stained with PAS, 0.5% toluidine blue and 1% azure II-methylene blue. Measurements were made from both SEM and Light micrographs.The tissue had two structurally distinct surfaces, an outer surface with small (225-500 µ) pubescent hairs (12/mm2), numerous stoma (77/mm2), and nectar glands(8/mm2); and an inner surface with large (784-1000 µ)stiff hairs(4/mm2), fewer stoma (46/mm2) and larger, more complex glands(16/mm2), presumably of a digestive nature.

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Modulation of endothelial inflammation after Methylene Blue: role of circulating cells

The Thoracic and Cardiovascular Surgeon ◽

10.1055/s-0034-1367260 ◽

2014 ◽

Vol 62 (S 01) ◽

Author(s):

I. Kanzler ◽

F. Guo ◽

N. Bogert ◽

A. Moritz ◽

A. Beiras-Fernandez

Keyword(s):

Methylene Blue ◽

Circulating Cells ◽

Endothelial Inflammation

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Role of heparan sulfate proteoglycans in the binding and uptake of apolipoprotein E-enriched remnant lipoproteins by cultured cells

Journal of Biological Chemistry ◽

10.1016/s0021-9258(18)82186-x ◽

1993 ◽

Vol 268 (14) ◽

pp. 10160-10167

Author(s):

Z.S. Ji ◽

W.J. Brecht ◽

R.D. Miranda ◽

M.M. Hussain ◽

T.L. Innerarity ◽

...

Keyword(s):

Apolipoprotein E ◽

Heparan Sulfate ◽

Cultured Cells ◽

Heparan Sulfate Proteoglycans ◽

Remnant Lipoproteins

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Possible Role of Peroxynitrite in the Responses Induced by Fusicoccin in Plant Cultured Cells

Plants ◽

10.3390/plants10010182 ◽

2021 ◽

Vol 10 (1) ◽

pp. 182 ◽

Cited By ~ 1

Author(s):

Massimo Malerba ◽

Raffaella Cerana

Keyword(s):

Cytochrome C ◽

Dna Fragmentation ◽

Stress Responses ◽

Molecular Chaperones ◽

Caspase 3 ◽

Cultured Cells ◽

Acer Pseudoplatanus ◽

Cytochrome C Release ◽

Induced Stress

Fusicoccin (FC) is a well-known phytotoxin able to induce in Acer pseudoplatanus L. (sycamore) cultured cells, a set of responses similar to those induced by stress conditions. In this work, the possible involvement of peroxynitrite (ONOO−) in FC-induced stress responses was studied measuring both in the presence and in the absence of 2,6,8-trihydroxypurine (urate), a specific ONOO− scavenger: (1) cell death; (2) specific DNA fragmentation; (3) lipid peroxidation; (4) production of RNS and ROS; (5) activity of caspase-3-like proteases; and (6) release of cytochrome c from mitochondria, variations in the levels of molecular chaperones Hsp90 in the mitochondria and Hsp70 BiP in the endoplasmic reticulum (ER), and of regulatory 14-3-3 proteins in the cytosol. The obtained results indicate a role for ONOO− in the FC-induced responses. In particular, ONOO− seems involved in a PCD form showing apoptotic features such as specific DNA fragmentation, caspase-3-like protease activity, and cytochrome c release from mitochondria.

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Metal Stress Response and Tolerance of Cultured Cells from Silene vulgaris and Lycopersicon peruvianum: Role of Heat Stress Proteins

Journal of Plant Physiology ◽

10.1016/s0176-1617(99)80296-x ◽

1999 ◽

Vol 154 (4) ◽

pp. 547-553 ◽

Cited By ~ 34

Author(s):

Reinhold Wollgiehn ◽

Dieter Neumann

Keyword(s):

Heat Stress ◽

Stress Response ◽

Stress Proteins ◽

Cultured Cells ◽

Metal Stress ◽

Lycopersicon Peruvianum ◽

Silene Vulgaris ◽

Heat Stress Proteins

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Hydrogen Peroxide Toxicity Induces Ras Signaling in Human Neuroblastoma SH-SY5Y Cultured Cells

Journal of Biomedicine and Biotechnology ◽

10.1155/2010/803815 ◽

2010 ◽

Vol 2010 ◽

pp. 1-4 ◽

Cited By ~ 7

Author(s):

Jirapa Chetsawang ◽

Piyarat Govitrapong ◽

Banthit Chetsawang

Keyword(s):

Hydrogen Peroxide ◽

Cell Viability ◽

Cultured Cells ◽

Neuronal Cells ◽

Competitive Inhibitor ◽

Ras Signaling ◽

Ras Proteins ◽

Human Neuroblastoma ◽

Dependent Signaling Pathway

It has been reported that overproduction of reactive oxygen species occurs after brain injury and mediates neuronal cells degeneration. In the present study, we examined the role of Ras signaling on hydrogen peroxide-induced neuronal cells degeneration in dopaminergic neuroblastoma SH-SY5Y cells. Hydrogen peroxide significantly reduced cell viability in SH-SY5Y cultured cells. An inhibitor of the enzyme that catalyzes the farnesylation of Ras proteins, FTI-277, and a competitive inhibitor of GTP-binding proteins, GDP-beta-S significantly decreased hydrogen peroxide-induced reduction in cell viability in SH-SY5Y cultured cells. The results of this study might indicate that a Ras-dependent signaling pathway plays a role in hydrogen peroxide-induced toxicity in neuronal cells.

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