Vis-NIR wavelength selection for non-destructive discriminant analysis of breed screening of transgenic sugarcane

2014 ◽  
Vol 6 (21) ◽  
pp. 8810-8816 ◽  
Author(s):  
Haosong Guo ◽  
Jiemei Chen ◽  
Tao Pan ◽  
Jihua Wang ◽  
Gan Cao

The Savitzky–Golay (SG) method and moving-window waveband screening are applied to a coupling model of principal component (PCA) and linear discriminant analyses (LDA).

2018 ◽  
Vol 11 (02) ◽  
pp. 1850005 ◽  
Author(s):  
Lijun Yao ◽  
Weiqun Xu ◽  
Tao Pan ◽  
Jiemei Chen

The moving-window bis-correlation coefficients (MW-BiCC) was proposed and employed for the discriminant analysis of transgenic sugarcane leaves and [Formula: see text]-thalassemia with visible and near-infrared (Vis–NIR) spectroscopy. The well-performed moving-window principal component analysis linear discriminant analysis (MW-PCA–LDA) was also conducted for comparison. A total of 306 transgenic (positive) and 150 nontransgenic (negative) leave samples of sugarcane were collected and divided to calibration, prediction, and validation. The diffuse reflection spectra were corrected using Savitzky–Golay (SG) smoothing with first-order derivative ([Formula: see text]), third-degree polynomial ([Formula: see text]) and 25 smoothing points ([Formula: see text]). The selected waveband was 736–1054[Formula: see text]nm with MW-BiCC, and the positive and negative validation recognition rates ([Formula: see text]_REC[Formula: see text], [Formula: see text]_REC[Formula: see text] were 100%, 98.0%, which achieved the same effect as MW-PCA–LDA. Another example, the 93 [Formula: see text]-thalassemia (positive) and 148 nonthalassemia (negative) of human hemolytic samples were collected. The transmission spectra were corrected using SG smoothing with [Formula: see text], [Formula: see text] and [Formula: see text]. Using MW-BiCC, many best wavebands were selected (e.g., 1116–1146, 1794–1848 and 2284–2342[Formula: see text]nm). The [Formula: see text]_REC[Formula: see text] and [Formula: see text]_REC[Formula: see text] were both 100%, which achieved the same effect as MW-PCA–LDA. Importantly, the BiCC only required calculating correlation coefficients between the spectrum of prediction sample and the average spectra of two types of calibration samples. Thus, BiCC was very simple in algorithm, and expected to obtain more applications. The results first confirmed the feasibility of distinguishing [Formula: see text]-thalassemia and normal control samples by NIR spectroscopy, and provided a promising simple tool for large population thalassemia screening.


Plant Disease ◽  
2012 ◽  
Vol 96 (11) ◽  
pp. 1683-1689 ◽  
Author(s):  
Sindhuja Sankaran ◽  
Reza Ehsani ◽  
Sharon A. Inch ◽  
Randy C. Ploetz

Laurel wilt, caused by the fungus Raffaelea lauricola, affects the growth, development, and productivity of avocado, Persea americana. This study evaluated the potential of visible-near infrared spectroscopy for non-destructive sensing of this disease. The symptoms of laurel wilt are visually similar to those caused by freeze damage (leaf necrosis). In this work, we performed classification studies with visible-near infrared spectra of asymptomatic and symptomatic leaves from infected plants, as well as leaves from freeze-damaged and healthy plants, both of which were non-infected. The principal component scores computed from principal component analysis were used as input features in four classifiers: linear discriminant analysis, quadratic discriminant analysis (QDA), Naïve-Bayes classifier, and bagged decision trees (BDT). Among the classifiers, QDA and BDT resulted in classification accuracies of higher than 94% when classifying asymptomatic leaves from infected plants. All of the classifiers were able to discriminate symptomatic-infected leaves from freeze-damaged leaves. However, the false negatives mainly resulted from asymptomatic-infected leaves being classified as healthy. Analyses of average vegetation indices of freeze-damaged, healthy (non-infected), asymptomatic-infected, and symptomatic-infected leaves indicated that the normalized difference vegetation index and the simple ratio index were statistically different.


2021 ◽  
pp. 096703352098731
Author(s):  
Adenilton C da Silva ◽  
Lívia PD Ribeiro ◽  
Ruth MB Vidal ◽  
Wladiana O Matos ◽  
Gisele S Lopes

The use of alcohol-based hand sanitizers is recommended as one of several strategies to minimize contamination and spread of the COVID-19 disease. Current reports suggest that the virucidal potential of ethanol occurs at concentrations close to 70%. Traditional methods of verifying the ethanol concentration in such products invite potential errors due to the viscosity of chemical components or may be prohibitively expensive to undertake in large demand. Near infrared (NIR) spectroscopy and chemometrics have already been used for the determination of ethanol in other matrices and present an alternative fast and reliable approach to quality control of alcohol-based hand sanitizers. In this study, a portable NIR spectrometer combined with classification chemometric tools, i.e., partial least square discriminant analysis (PLS–DA) and linear discriminant analysis with successive algorithm projection (SPA–LDA) were used to construct models to identify conforming and non-conforming commercial and laboratory synthesized hand sanitizer samples. Principal component analysis (PCA) was applied in an exploratory data study. Three principal components accounted for 99% of data variance and demonstrate clustering of conforming and non-conforming samples. The PLS–DA and SPA–LDA classification models presented 77 and 100% of accuracy in cross/internal validation respectively and 100% of accuracy in the classification of test samples. A total of 43% commercial samples evaluated using the PLS–DA and SPA–LDA presented ethanol content non-conforming for hand sanitizer gel. These results indicate that use of NIR spectroscopy and chemometrics is a promising strategy, yielding a method that is fast, portable, and reliable for discrimination of alcohol-based hand sanitizers with respect to conforming and non-conforming ethanol concentrations.


Sensors ◽  
2018 ◽  
Vol 18 (12) ◽  
pp. 4479 ◽  
Author(s):  
Xavier Cetó ◽  
Núria Serrano ◽  
Miriam Aragó ◽  
Alejandro Gámez ◽  
Miquel Esteban ◽  
...  

The development of a simple HPLC-UV method towards the evaluation of Spanish paprika’s phenolic profile and their discrimination based on the former is reported herein. The approach is based on C18 reversed-phase chromatography to generate characteristic fingerprints, in combination with linear discriminant analysis (LDA) to achieve their classification. To this aim, chromatographic conditions were optimized so as to achieve the separation of major phenolic compounds already identified in paprika. Paprika samples were subjected to a sample extraction stage by sonication and centrifugation; extracting procedure and conditions were optimized to maximize the generation of enough discriminant fingerprints. Finally, chromatograms were baseline corrected, compressed employing fast Fourier transform (FFT), and then analyzed by means of principal component analysis (PCA) and LDA to carry out the classification of paprika samples. Under the developed procedure, a total of 96 paprika samples were analyzed, achieving a classification rate of 100% for the test subset (n = 25).


Author(s):  
David Zhang ◽  
Xiao-Yuan Jing ◽  
Jian Yang

This chapter presents two straightforward image projection techniques — two-dimensional (2D) image matrix-based principal component analysis (IMPCA, 2DPCA) and 2D image matrix-based Fisher linear discriminant analysis (IMLDA, 2DLDA). After a brief introduction, we first introduce IMPCA. Then IMLDA technology is given. As a result, we summarize some useful conclusions.


Electronics ◽  
2019 ◽  
Vol 8 (8) ◽  
pp. 870
Author(s):  
Tengteng Wen ◽  
Dehan Luo ◽  
Yongjie Ji ◽  
Pingzhong Zhong

Odor reproduction, a branch of machine olfaction, is a technology through which a machine represents various odors by blending several odor sources in different proportions and releases them. In this paper, an odor reproduction system is proposed. The system includes an atomization-based odor dispenser using 16 micro-porous piezoelectric transducers. The authors propose the use of an electronic nose combined with a Principal Component Analysis–Linear Discriminant Analysis (PCA–LDA) model to evaluate the effectiveness of the system. The results indicate that the model can be used to evaluate the system.


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