An EWOD-based microfluidic chip for single-cell isolation, mRNA purification and subsequent multiplex qPCR

Lab on a Chip ◽  
2014 ◽  
Vol 14 (19) ◽  
pp. 3739-3749 ◽  
Author(s):  
A. Rival ◽  
D. Jary ◽  
C. Delattre ◽  
Y. Fouillet ◽  
G. Castellan ◽  
...  
Keyword(s):  
Gene Expression ◽  
Sample Preparation ◽  
Single Cell ◽  
Microfluidic Chip ◽  
Gene Expression Analysis ◽  
Magnetic Beads ◽  
Cell Manipulation ◽  
Qrt Pcr ◽  
Single Cell Isolation ◽  
Digital Microfluidic

A compact EWOD digital microfluidic chip enables single cell manipulation, sample preparation using magnetic beads and gene expression analysis by qRT-PCR.

scholarly journals Using single-cell transcriptomics to understand functional states and interactions in microbial eukaryotes

2019 ◽  
Vol 374 (1786) ◽  
pp. 20190098 ◽  
Author(s):  
Chuan Ku ◽  
Arnau Sebé-Pedrós
Keyword(s):  
Gene Expression ◽  
Single Cell ◽  
Expression Analysis ◽  
Gene Expression Analysis ◽  
Interspecific Interactions ◽  
Single Cells ◽  
Life Cycles ◽  
Modern Biology ◽  
Microbial Eukaryotes ◽  
Eukaryotic Microorganisms

Understanding the diversity and evolution of eukaryotic microorganisms remains one of the major challenges of modern biology. In recent years, we have advanced in the discovery and phylogenetic placement of new eukaryotic species and lineages, which in turn completely transformed our view on the eukaryotic tree of life. But we remain ignorant of the life cycles, physiology and cellular states of most of these microbial eukaryotes, as well as of their interactions with other organisms. Here, we discuss how high-throughput genome-wide gene expression analysis of eukaryotic single cells can shed light on protist biology. First, we review different single-cell transcriptomics methodologies with particular focus on microbial eukaryote applications. Then, we discuss single-cell gene expression analysis of protists in culture and what can be learnt from these approaches. Finally, we envision the application of single-cell transcriptomics to protist communities to interrogate not only community components, but also the gene expression signatures of distinct cellular and physiological states, as well as the transcriptional dynamics of interspecific interactions. Overall, we argue that single-cell transcriptomics can significantly contribute to our understanding of the biology of microbial eukaryotes. This article is part of a discussion meeting issue ‘Single cell ecology’.

scholarly journals Resolution of Cell Fate Decisions Revealed by Single-Cell Gene Expression Analysis from Zygote to Blastocyst

2010 ◽  
Vol 18 (4) ◽  
pp. 675-685 ◽  
Author(s):  
Guoji Guo ◽  
Mikael Huss ◽  
Guo Qing Tong ◽  
Chaoyang Wang ◽  
Li Li Sun ◽  
...  
Keyword(s):  
Gene Expression ◽  
Single Cell ◽  
Cell Fate ◽  
Expression Analysis ◽  
Gene Expression Analysis ◽  
Cell Fate Decisions ◽  
Cell Gene Expression ◽  
Cell Gene

An integrated digital microfluidic chip for multiplexed proteomic sample preparation and analysis by MALDI-MS

Lab on a Chip ◽  
2006 ◽  
Vol 6 (9) ◽  
pp. 1213 ◽  
Author(s):  
Hyejin Moon ◽  
Aaron R. Wheeler ◽  
Robin L. Garrell ◽  
Joseph A. Loo ◽  
Chang-Jin ?CJ? Kim
Keyword(s):  
Sample Preparation ◽  
Microfluidic Chip ◽  
Maldi Ms ◽  
Digital Microfluidic

scholarly journals Characterization of transcriptional networks in blood stem and progenitor cells using high-throughput single-cell gene expression analysis

2013 ◽  
Vol 15 (4) ◽  
pp. 363-372 ◽  
Author(s):  
Victoria Moignard ◽  
Iain C. Macaulay ◽  
Gemma Swiers ◽  
Florian Buettner ◽  
Judith Schütte ◽  
...  
Keyword(s):  
Gene Expression ◽  
Single Cell ◽  
Progenitor Cells ◽  
High Throughput ◽  
Gene Expression Analysis ◽  
Transcriptional Networks ◽  
Stem And Progenitor Cells ◽  
Cell Gene Expression ◽  
Cell Gene
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