Bioactivated protein-based porous microcarriers for tissue engineering applications

2014 ◽  
Vol 2 (44) ◽  
pp. 7795-7803 ◽  
Author(s):  
Baiwen Luo ◽  
Qiu Li Loh ◽  
Marcus Thien Chong Wong ◽  
Nguan Soon Tan ◽  
Cleo Choong
Keyword(s):  
Tissue Engineering ◽  
Extracellular Matrix ◽  
Cell Adhesion ◽  
Biological Factors ◽  
Adhesion Receptors ◽  
Engineering Applications ◽  
Cell Material ◽  
Cell Material Interactions

Lipoaspirate-derived extracellular matrix enrichment was able to provide the necessary cell adhesion receptors and biological factors for improving cell–material interactions of porous OVA microcarriers.

Evaluation of Cell-Material Interactions on Newly Designed, Printable Polymers for Tissue Engineering Applications

2011 ◽  
Vol 13 (12) ◽  
pp. B467-B475 ◽  
Author(s):  
Esther C. Novosel ◽  
Wolfdietrich Meyer ◽  
Nadine Klechowitz ◽  
Hartmut Krüger ◽  
Michael Wegener ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Engineering Applications ◽  
Cell Material ◽  
Cell Material Interactions

Decellularized natural 3D cellulose scaffold derived from Borassus flabellifer (Linn.) as extracellular matrix for tissue engineering applications

2021 ◽  
pp. 118494
Author(s):  
Balaji Mahendiran ◽  
Shalini Muthusamy ◽  
R. Selvakumar ◽  
Narmadha Rajeswaran ◽  
Sowndarya Sampath ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Extracellular Matrix ◽  
Engineering Applications

scholarly journals Nanometer-sized extracellular matrix coating on polymer-based scaffold for tissue engineering applications

2015 ◽  
Vol 104 (1) ◽  
pp. 94-103 ◽  
Author(s):  
Noriyuki Uchida ◽  
Srikanth Sivaraman ◽  
Nicholas J. Amoroso ◽  
William R. Wagner ◽  
Akihiro Nishiguchi ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Extracellular Matrix ◽  
Engineering Applications

scholarly journals Fabrication of Nanohydroxyapatite/Poly(caprolactone) Composite Microfibers Using Electrospinning Technique for Tissue Engineering Applications

2014 ◽  
Vol 2014 ◽  
pp. 1-7 ◽  
Author(s):  
Mohd Izzat Hassan ◽  
Tao Sun ◽  
Naznin Sultana
Keyword(s):  
Tissue Engineering ◽  
Extracellular Matrix ◽  
Three Dimensional ◽  
Average Diameter ◽  
Tissue Engineering Scaffold ◽  
Engineering Applications ◽  
Electrospinning Technique ◽  
Fibrous Scaffolds ◽  
Nanosized Hydroxyapatite ◽  
Poly Caprolactone

Tissue engineering fibrous scaffolds serve as three-dimensional (3D) environmental framework by mimicking the extracellular matrix (ECM) for cells to grow. Biodegradable polycaprolactone (PCL) microfibers were fabricated to mimic the ECM as a scaffold with 7.5% (w/v) and 12.5% (w/v) concentrations. Lower PCL concentration of 7.5% (w/v) resulted in microfibers with bead defects. The average diameter of fibers increased at higher voltage and the distance of tip to collector. Further investigation was performed by the incorporation of nanosized hydroxyapatite (nHA) into microfibers. The incorporation of 10% (w/w) nHA with 7.5% (w/v) PCL solution produced submicron sized beadless fibers. The microfibrous scaffolds were evaluated using various techniques. Biodegradable PCL and nHA/PCL could be promising for tissue engineering scaffold application.

Quantifying Mechanical Properties of PCL-Based Nanofiber Mats Using Atomic Force Microscopy

2019 ◽  
Author(s):  
Allison White ◽  
Amanda DeVos ◽  
Amr Elamin Elhussein ◽  
Jack Blank ◽  
Kalyani Nair
Keyword(s):  
Mechanical Properties ◽  
Tissue Engineering ◽  
Cell Proliferation ◽  
Cell Adhesion ◽  
Chemical Properties ◽  
Cell Behavior ◽  
Engineering Applications ◽  
Collagen Coating ◽  
Atomic Force ◽  
Nanofiber Mats

Abstract Polymeric scaffolds aid in creating an environment for cell proliferation and differentiation in tissue engineering applications by acting as temporary artificial extracellular matrices (ECMs) for cells to form functional tissue. Many studies have reported that cell behavior can be significantly affected by the physical and chemical properties of a given scaffold. Therefore, the mechanical and structural properties of these scaffolds must be characterized. Polymeric solutions, such as polycaprolactone (PCL), have been electrospun into nanofiber mats to be used as cell scaffolds. Polycaprolactone (PCL) is a biocompatible polymer and is commonly used in tissue engineering applications; however, PCL is hydrophobic, which makes it difficult for cells to adhere to the mat. Coating the PCL-based mats with collagen, a naturally occurring protein with hydrophilic properties, may improve cell adhesion to the scaffold. The collagen coating may also alter the mechanical properties of the nanofiber mats. In this study, the effect of collagen coating on cell adhesion and proliferation are investigated using alamarBlue tests. Additionally, the mechanical and surface properties of PCL-based nanofiber mats are investigated using a Nanosurf C3000 atomic force microscope (AFM). One batch of PCL mats were coated with collagen, while the uncoated mats were used as controls. The cell behavior and material property values obtained from the uncoated PCL and collagen-coated PCL mats were analyzed and compared. The results of this study suggest that collagen does significantly influence the cell proliferation and material properties of PCL-based mats and that further studies should be conducted to better understand the effects of the nanoscale properties of the PCL-based mats on cell adhesion.

Cell Adhesion on a Wavy Surface

2013 ◽  
Author(s):  
Jia Hu ◽  
Yaling Liu
Keyword(s):  
Tissue Engineering ◽  
Cell Adhesion ◽  
Cell Response ◽  
Cell Spreading ◽  
Wavy Surface ◽  
Nanostructured Surface ◽  
Engineering Applications ◽  
Micro Scale ◽  
Nano Scale ◽  
A Cell

The ability to control the position of cells in an organized pattern on a substrate has become increasingly important for biosensing and tissue engineering applications [1–3]. With the advent of nanofabrication techniques, a number of researchers have studied the effects of nano-scale grooves on cell spreading, migration, morphology, signaling and orientation [4–6]. Recent studies have shown that cell adhesion/spreading can be influenced by a nanostructured surface [7]. In most current studies, the pattern dimensions are much smaller than the size of a cell. In this paper, we focus on studying cell response to micro scale patterns instead of nano-scale patterns.

A review on 3D printing in tissue engineering applications

2022 ◽  
Vol 0 (0) ◽  
Author(s):  
Mohan Prasath Mani ◽  
Madeeha Sadia ◽  
Saravana Kumar Jaganathan ◽  
Ahmad Zahran Khudzari ◽  
Eko Supriyanto ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Extracellular Matrix ◽  
3D Printing ◽  
Cell Adherence ◽  
Engineering Applications ◽  
Proliferation And Differentiation ◽  
3D Printed ◽  
Printing Techniques

Abstract In tissue engineering, 3D printing is an important tool that uses biocompatible materials, cells, and supporting components to fabricate complex 3D printed constructs. This review focuses on the cytocompatibility characteristics of 3D printed constructs, made from different synthetic and natural materials. From the overview of this article, inkjet and extrusion-based 3D printing are widely used methods for fabricating 3D printed scaffolds for tissue engineering. This review highlights that scaffold prepared by both inkjet and extrusion-based 3D printing techniques showed significant impact on cell adherence, proliferation, and differentiation as evidenced by in vitro and in vivo studies. 3D printed constructs with growth factors (FGF-2, TGF-β1, or FGF-2/TGF-β1) enhance extracellular matrix (ECM), collagen I content, and high glycosaminoglycan (GAG) content for cell growth and bone formation. Similarly, the utilization of 3D printing in other tissue engineering applications cannot be belittled. In conclusion, it would be interesting to combine different 3D printing techniques to fabricate future 3D printed constructs for several tissue engineering applications.

scholarly journals Protein/polysaccharide-based scaffolds mimicking native extracellular matrix for cardiac tissue engineering applications

2017 ◽  
Vol 106 (3) ◽  
pp. 769-781 ◽  
Author(s):  
Elisabetta Rosellini ◽  
Yu Shrike Zhang ◽  
Bianca Migliori ◽  
Niccoletta Barbani ◽  
Luigi Lazzeri ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Extracellular Matrix ◽  
Cardiac Tissue ◽  
Cardiac Tissue Engineering ◽  
Engineering Applications
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