Quantifying bacterial adhesion on antifouling polymer brushes via single-cell force spectroscopy

2015 ◽  
Vol 6 (31) ◽  
pp. 5740-5751 ◽  
Author(s):  
Cesar Rodriguez-Emmenegger ◽  
Sébastien Janel ◽  
Andres de los Santos Pereira ◽  
Michael Bruns ◽  
Frank Lafont
Keyword(s):  
Atomic Force Microscope ◽  
Single Cell ◽  
Bacterial Adhesion ◽  
Bacterial Cell ◽  
Polymer Brushes ◽  
Force Spectroscopy ◽  
Adhesion Forces ◽  
Atomic Force ◽  
Cell Force

The adhesion forces between a single bacterial cell and different polymer brushes were measured directly with an atomic force microscope and correlated with their resistance to fouling.

scholarly journals FluidFM as a Tool to Study Adhesion Forces of Bacteria - Optimization of Parameters and Comparison to Conventional Bacterial Probe Scanning Force Spectroscopy

2019 ◽  
Author(s):  
Linda Hofherr ◽  
Christine Müller-Renno ◽  
Christiane Ziegler
Keyword(s):  
Single Cell ◽  
Single Cells ◽  
Force Spectroscopy ◽  
Bacterial Cells ◽  
Chemical Fixation ◽  
Adhesion Forces ◽  
Optimization Of Parameters ◽  
Scanning Force ◽  
Cell Force

AbstractThe FluidFM enables the immobilization of single cells on a hollow cantilever using relative underpressure. In this study, we systematically optimize versatile measurement parameters to improve the quality of force-distance curves recorded with a FluidFM. Using single bacterial cells, we show that Single Cell Force Spectroscopy experiments with the FluidFM lead to comparable results to a conventional Single Cell Force Spectroscopy approach using polydopamine for chemical fixation of a bacterial cell.

scholarly journals Probing the Adhesion Forces of Staphylococcus aureus to Central Venous Catheters by Single-Cell Force Spectroscopy

2019 ◽  
Vol 25 (S1) ◽  
pp. 53-54
Author(s):  
Gubesh Gunaratnam ◽  
Philipp Jung ◽  
Jacqueline Willeit ◽  
Christian Spengler ◽  
Karin Jacobs ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Single Cell ◽  
Force Spectroscopy ◽  
Central Venous Catheters ◽  
Adhesion Forces ◽  
Central Venous ◽  
Cell Force

In Situ Quantification of Living Cell Adhesion Forces: Single Cell Force Spectroscopy with a Nanotweezer

Langmuir ◽  
2014 ◽  
Vol 30 (10) ◽  
pp. 2952-2959 ◽  
Author(s):  
Hui Xie ◽  
Munan Yin ◽  
Weibin Rong ◽  
Lining Sun
Keyword(s):  
Cell Adhesion ◽  
Single Cell ◽  
Living Cell ◽  
Force Spectroscopy ◽  
Adhesion Forces ◽  
Cell Force

scholarly journals Investigation of Adhesion and Mechanical Properties of Human Glioma Cells by Single Cell Force Spectroscopy and Atomic Force Microscopy

PLoS ONE ◽  
2014 ◽  
Vol 9 (11) ◽  
pp. e112582 ◽  
Author(s):  
Laura Andolfi ◽  
Eugenia Bourkoula ◽  
Elisa Migliorini ◽  
Anita Palma ◽  
Anja Pucer ◽  
...  
Keyword(s):  
Mechanical Properties ◽  
Atomic Force Microscopy ◽  
Single Cell ◽  
Glioma Cells ◽  
Force Spectroscopy ◽  
Human Glioma ◽  
Human Glioma Cells ◽  
Force Microscopy ◽  
Atomic Force ◽  
Cell Force

scholarly journals Effect of Graphene Oxide Packing on Bacterial Adhesion using Single Cell Force Spectroscopy

2018 ◽  
Vol 114 (3) ◽  
pp. 352a-353a
Author(s):  
Elise Linna ◽  
Sara BinAhmed ◽  
Benjamin L. Stottrup ◽  
Santiago Romero-Vargas Castrill¢n
Keyword(s):  
Graphene Oxide ◽  
Single Cell ◽  
Bacterial Adhesion ◽  
Force Spectroscopy ◽  
Cell Force
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