Functionalization of Atomic Force Microscope Cantilevers with Single-T Cells or Single-Particle for Immunological Single-Cell Force Spectroscopy

10.3791/59609 ◽  
2019 ◽  
Author(s):  
Jiahuan Chen ◽  
Ying Xu ◽  
Yan Shi ◽  
Tie Xia
Keyword(s):  
T Cells ◽  
Atomic Force Microscope ◽  
Single Cell ◽  
Single Particle ◽  
Force Spectroscopy ◽  
Atomic Force ◽  
Cell Force

Quantifying bacterial adhesion on antifouling polymer brushes via single-cell force spectroscopy

2015 ◽  
Vol 6 (31) ◽  
pp. 5740-5751 ◽  
Author(s):  
Cesar Rodriguez-Emmenegger ◽  
Sébastien Janel ◽  
Andres de los Santos Pereira ◽  
Michael Bruns ◽  
Frank Lafont
Keyword(s):  
Atomic Force Microscope ◽  
Single Cell ◽  
Bacterial Adhesion ◽  
Bacterial Cell ◽  
Polymer Brushes ◽  
Force Spectroscopy ◽  
Adhesion Forces ◽  
Atomic Force ◽  
Cell Force

The adhesion forces between a single bacterial cell and different polymer brushes were measured directly with an atomic force microscope and correlated with their resistance to fouling.

scholarly journals Investigation of Adhesion and Mechanical Properties of Human Glioma Cells by Single Cell Force Spectroscopy and Atomic Force Microscopy

PLoS ONE ◽  
2014 ◽  
Vol 9 (11) ◽  
pp. e112582 ◽  
Author(s):  
Laura Andolfi ◽  
Eugenia Bourkoula ◽  
Elisa Migliorini ◽  
Anita Palma ◽  
Anja Pucer ◽  
...  
Keyword(s):  
Mechanical Properties ◽  
Atomic Force Microscopy ◽  
Single Cell ◽  
Glioma Cells ◽  
Force Spectroscopy ◽  
Human Glioma ◽  
Human Glioma Cells ◽  
Force Microscopy ◽  
Atomic Force ◽  
Cell Force

scholarly journals Single cell force spectroscopy of T cells recognizing a myelin-derived peptide on antigen presenting cells

2011 ◽  
Vol 136 (1) ◽  
pp. 13-20 ◽  
Author(s):  
Sabrina Hoffmann ◽  
Babak H. Hosseini ◽  
Markus Hecker ◽  
Ilia Louban ◽  
Nadja Bulbuc ◽  
...  
Keyword(s):  
T Cells ◽  
Single Cell ◽  
Force Spectroscopy ◽  
Antigen Presenting Cells ◽  
Antigen Presenting ◽  
Cell Force

scholarly journals Probing the PEDOT:PSS/cell interface with conductive colloidal probe AFM-SECM

Nanoscale ◽  
2016 ◽  
Vol 8 (8) ◽  
pp. 4475-4481 ◽  
Author(s):  
P. Knittel ◽  
H. Zhang ◽  
C. Kranz ◽  
G. G. Wallace ◽  
M. J. Higgins
Keyword(s):  
Electrical Stimulation ◽  
Single Cell ◽  
Molecular Level ◽  
Force Spectroscopy ◽  
Scanning Electrochemical Microscopy ◽  
Colloidal Probe ◽  
Atomic Force ◽  
Cell Force ◽  
Cell Interface ◽  
Electrochemical Microscopy

We introduce a novel conductive colloidal Atomic Force-Scanning Electrochemical Microscopy (AFM-SECM) probe for single cell force spectroscopy that enables detection of single cell and molecular-level force interactions at electromaterial interfaces whilst applying electrical stimulation.

Atomic force microscope-based single-molecule force spectroscopy of RNA unfolding

2011 ◽  
Vol 414 (1) ◽  
pp. 1-6 ◽  
Author(s):  
Hans A. Heus ◽  
Elias M. Puchner ◽  
Aafke J. van Vugt-Jonker ◽  
Julia L. Zimmermann ◽  
Hermann E. Gaub
Keyword(s):  
Atomic Force Microscope ◽  
Single Molecule ◽  
Force Spectroscopy ◽  
Single Molecule Force Spectroscopy ◽  
Atomic Force
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