Scanning fluorescence correlation spectroscopy as a versatile tool to measure static and dynamic properties of soft matter systems

Soft Matter ◽  
2015 ◽  
Vol 11 (46) ◽  
pp. 8939-8947
Author(s):  
Manish Nepal ◽  
Alon Oyler-Yaniv ◽  
Oleg Krichevsky
Keyword(s):  
Fluorescence Correlation Spectroscopy ◽  
Soft Matter ◽  
Dynamic Properties ◽  
Correlation Spectroscopy ◽  
Fluorescence Labeling ◽  
Fluorescence Correlation ◽  
Specific Fluorescence ◽  
System P ◽  
Versatile Tool ◽  
Matter System

Scanning fluorescence correlation spectroscopy in combination with specific fluorescence labeling is used to measure different static and dynamic properties of a soft matter system.

Fluorescence correlation spectroscopy: The technique and its applications in soft matter

2018 ◽  
Vol 4 (4) ◽  
Author(s):  
Anjali Gupta ◽  
Jagadish Sankaran ◽  
Thorsten Wohland
Keyword(s):  
Single Molecule ◽  
Fluorescence Correlation Spectroscopy ◽  
Soft Matter ◽  
Photophysical Properties ◽  
Spatiotemporal Analysis ◽  
Correlation Spectroscopy ◽  
Basic Principles ◽  
Chemical Reaction Kinetics ◽  
Fluorescence Correlation ◽  
Wide Range

Abstract Fluorescence correlation spectroscopy (FCS) is a well-established single-molecule method used for the quantitative spatiotemporal analysis of dynamic processes in a wide range of samples. It possesses single-molecule sensitivity but provides ensemble averaged molecular parameters such as mobility, concentration, chemical reaction kinetics, photophysical properties and interaction properties. These parameters have been utilized to characterize a variety of soft matter systems. This review provides an overview of the basic principles of various FCS modalities, their instrumentation, data analysis, and the applications of FCS to soft matter systems.

scholarly journals Development of a fluorescence correlation spectroscopy instrument and its application in sizing quantum dot nanoparticles

2015 ◽  
Vol 25 (1) ◽  
pp. 59 ◽  
Author(s):  
Nguyen Thi Thanh Bao ◽  
Dinh Van Trung
Keyword(s):  
Quantum Dots ◽  
Fluorescence Correlation Spectroscopy ◽  
Dynamic Properties ◽  
Correlation Spectroscopy ◽  
Fluorescence Signal ◽  
Fluorescence Correlation ◽  
Statistical Fluctuations ◽  
Measurement Results ◽  
Particle Level ◽  
Microscope Imaging

Fluorescence correlation spectroscopy is a relatively new technique to measure and quantify the statistical fluctuations of the fluorescence signal from the measurement volume. Combining with sensitive detection method and confocal microscopy, the FCS technique has become a powerful tool in studying the dynamic properties of nanoparticles at single particle level. In this paper we present the construction of a highly sensitive FCS instrument and the measurement results from a sample of semiconductor quantum dots. We provide the analysis procedure for determining the hydrodynamic radius of the quantum dots and compare the results with that obtained directly from electron microscope imaging. The good agreement indicates the reliability of the FCS technique and open the way for further applications of this technique in studying nanoparticles.

Fluorescence correlation spectroscopy study of the complexation of DNA hybrids, IgG antibody, and a chimeric protein of IgG-binding ZZ domains fused with a carbohydrate binding module

2017 ◽  
Vol 19 (25) ◽  
pp. 16606-16614 ◽  
Author(s):  
A. M. M. Rosa ◽  
D. M. F. Prazeres ◽  
P. M. R. Paulo
Keyword(s):  
Fluorescence Correlation Spectroscopy ◽  
Dna Recognition ◽  
Chimeric Protein ◽  
Recognition System ◽  
Correlation Spectroscopy ◽  
Carbohydrate Binding ◽  
Igg Antibody ◽  
Fluorescence Correlation ◽  
System P ◽  
Igg Binding

Fluorescence correlation spectroscopy (FCS) was used to characterize the molecular interactions between the four components of a DNA recognition system.

scholarly journals Nano-antenna enhanced two-focus fluorescence correlation spectroscopy

2017 ◽  
Vol 7 (1) ◽  
Author(s):  
Lutz Langguth ◽  
Agata Szuba ◽  
Sander A. Mann ◽  
Erik C. Garnett ◽  
Gijsje H. Koenderink ◽  
...  
Keyword(s):  
Fluorescence Correlation Spectroscopy ◽  
Correlation Spectroscopy ◽  
Fluorescence Correlation
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