High-throughput sialylation measurement using lectins on an Octet platform for clone screening

Abstract Insecticide resistance has recently become a serious problem in the agricultural field. Development of insecticides with new mechanisms of action is essential to overcome this limitation. Juvenile hormone (JH) is an insect-specific hormone that plays key roles in maintaining the larval stage of insects. Hence, JH signaling pathway is considered a suitable target in the development of novel insecticides; however, only a few JH signaling inhibitors (JHSIs) have been reported, and no practical JHSIs have been developed. Here, we established a high-throughput screening (HTS) system for exploration of novel JHSIs using a Bombyx mori cell line (BmN_JF&AR cells) and carried out a large-scale screening in this cell line using a chemical library. The four-step HTS yielded 69 compounds as candidate JHSIs. Topical application of JHSI48 to B. mori larvae caused precocious metamorphosis. In ex vivo culture of the epidermis, JHSI48 suppressed the expression of the Krüppel homolog 1 gene, which is directly activated by JH-liganded receptor. Moreover, JHSI48 caused a parallel rightward shift in the JH response curve, suggesting that JHSI48 possesses a competitive antagonist-like activity. Thus, large-scale HTS using chemical libraries may have applications in development of future insecticides targeting the JH signaling pathway.

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An improved high-throughput method for assessing the impact of bioformulation on plant parameters: Case study with pigeonpea seeds

Physiological and Molecular Plant Pathology ◽

10.1016/j.pmpp.2021.101657 ◽

2021 ◽

pp. 101657

Author(s):

Sakshi Tewari ◽

Shilpi Sharma

Keyword(s):

High Throughput ◽

High Throughput Method ◽

The Impact

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High Throughput Centrifugal Electrospinning of Polyacrylonitrile Nanofibers for Carbon Fiber Nonwovens

Polymers ◽

10.3390/polym13081313 ◽

2021 ◽

Vol 13 (8) ◽

pp. 1313

Author(s):

Andreas Hoffmann ◽

Alexander J. C. Kuehne

Keyword(s):

High Throughput ◽

Carbon Nanofiber ◽

Fiber Diameter ◽

Carbon Precursor ◽

Production Methods ◽

High Throughput Method ◽

Radial Forces ◽

Fiber Deposition ◽

The Impact ◽

Pan Fibers

Carbon nanofiber nonwovens are promising materials for electrode or filtration applications; however, their utilization is obviated by a lack of high throughput production methods. In this study, we utilize a highly effective high-throughput method for the fabrication of polyacrylonitrile (PAN) nanofibers as a nonwoven on a dedicated substrate. The method employs rotational-, air pressure- and electrostatic forces to produce fibers from the inner edge of a rotating bell towards a flat collector. We investigate the impact of all above-mentioned forces on the fiber diameter, morphology, and bundling of the carbon-precursor PAN fibers. The interplay of radial forces with collector-facing forces has an influence on the uniformity of fiber deposition. Finally, the obtained PAN nanofibers are converted to carbon nonwovens by thermal treatment.

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