scholarly journals Rapid protein immobilization for thin film continuous flow biocatalysis

2016 ◽  
Vol 52 (66) ◽  
pp. 10159-10162 ◽  
Author(s):  
Joshua Britton ◽  
Colin L. Raston ◽  
Gregory A. Weiss
Keyword(s):  
Thin Film ◽  
Enzyme Activity ◽  
Enzyme Immobilization ◽  
Continuous Flow ◽  
Immobilized Enzyme ◽  
Protein Immobilization

Continuous flow biocatalysis gets a new spin. An efficient and general enzyme immobilization technique for vortex fluidic processing has been developed. The immobilized enzyme demonstrated no decrease in enzyme activity over 10 h in continuous flow with a >95% reduction in quantities of required reagents and enzymes.

Enzyme Immobilization on Nanomaterials for Biosensor and Biocatalyst in Food and Biomedical Industry

2019 ◽  
Vol 25 (24) ◽  
pp. 2661-2676 ◽  
Author(s):  
Sundaresan Bhavaniramya ◽  
Ramar Vanajothi ◽  
Selvaraju Vishnupriya ◽  
Kumpati Premkumar ◽  
Mohammad S. Al-Aboody ◽  
...  
Keyword(s):  
Enzyme Activity ◽  
Enzyme Immobilization ◽  
Food Industry ◽  
Environmental Changes ◽  
Immobilized Enzymes ◽  
Food Industries ◽  
Physiological Processes ◽  
Different Types ◽  
Simple Processing ◽  
Biomedical Industry

Enzymes exhibit a great catalytic activity for several physiological processes. Utilization of immobilized enzymes has a great potential in several food industries due to their excellent functional properties, simple processing and cost effectiveness during the past decades. Though they have several applications, they still exhibit some challenges. To overcome the challenges, nanoparticles with their unique physicochemical properties act as very attractive carriers for enzyme immobilization. The enzyme immobilization method is not only widely used in the food industry but is also a component methodology in the pharmaceutical industry. Compared to the free enzymes, immobilized forms are more robust and resistant to environmental changes. In this method, the mobility of enzymes is artificially restricted to changing their structure and properties. Due to their sensitive nature, the classical immobilization methods are still limited as a result of the reduction of enzyme activity. In order to improve the enzyme activity and their properties, nanomaterials are used as a carrier for enzyme immobilization. Recently, much attention has been directed towards the research on the potentiality of the immobilized enzymes in the food industry. Hence, the present review emphasizes the different types of immobilization methods that is presently used in the food industry and other applications. Various types of nanomaterials such as nanofibers, nanoflowers and magnetic nanoparticles are significantly used as a support material in the immobilization methods. However, several numbers of immobilized enzymes are used in the food industries to improve the processing methods which not only reduce the production cost but also the effluents from the industry.

scholarly journals Effects of morphology and pore size of mesoporous silicas on the efficiency of an immobilized enzyme

RSC Advances ◽  
2021 ◽  
Vol 11 (17) ◽  
pp. 10010-10017
Author(s):  
Ping-Chung Kuo ◽  
Zhi-Xun Lin ◽  
Tzi-Yi Wu ◽  
Chun-Han Hsu ◽  
Hong-Ping Lin ◽  
...  
Keyword(s):  
Enzyme Activity ◽  
Mesoporous Silica ◽  
Pore Size ◽  
Immobilized Enzyme ◽  
Mesoporous Silicas ◽  
High Loading ◽  
Loading Capacity ◽  
Silica Films

Mesoporous silica films were used as supports with high loading capacity and enzyme activity.

Protein Immobilization in Metal–Organic Frameworks by Covalent Binding

2014 ◽  
Vol 67 (11) ◽  
pp. 1629 ◽  
Author(s):  
Xuan Wang ◽  
Trevor A. Makal ◽  
Hong-Cai Zhou
Keyword(s):  
Enzymatic Activity ◽  
Enzyme Immobilization ◽  
Working Conditions ◽  
Active Site ◽  
Covalent Binding ◽  
Metal Organic Frameworks ◽  
Protein Immobilization ◽  
Immobilization Of Enzymes ◽  
Metal Organic ◽  
Biological Catalysis

Metal–organic frameworks (MOFs), possessing a well defined system of pores, demonstrate extensive potential serving as a platform in biological catalysis. Successful immobilization of enzymes in a MOF system retains the enzymatic activity, renders the active site more accessible to the substrate, and promises recyclability for reuse, and solvent adaptability in a broad range of working conditions. This highlight describes enzyme immobilization on MOFs via covalent binding and its significance.

Kinetic study on degradation of gaseous acetone over thin-film TiO2 photocatalyst in a continuous flow system

2007 ◽  
Vol 90 (1) ◽  
pp. 85-91 ◽  
Author(s):  
Sung Hyun Kim ◽  
Sang Bum Kim ◽  
Gyung Soo Kim ◽  
Hyun Tae Jang ◽  
Sung Chang Hong
Keyword(s):  
Thin Film ◽  
Kinetic Study ◽  
Continuous Flow ◽  
Flow System ◽  
Tio2 Photocatalyst ◽  
Continuous Flow System

scholarly journals Turbo thin film continuous flow production of biodiesel from fungal biomass

2019 ◽  
Vol 273 ◽  
pp. 431-438 ◽  
Author(s):  
Eko K. Sitepu ◽  
Darryl B. Jones ◽  
Zhanying Zhang ◽  
Youhong Tang ◽  
Sophie C. Leterme ◽  
...  
Keyword(s):  
Thin Film ◽  
Continuous Flow ◽  
Fungal Biomass

Moving into Nanotechnology Roles to Mimic and Boost Enzyme Activity

2018 ◽  
pp. 421-440
Author(s):  
Maria Laura Soriano
Keyword(s):  
Enzyme Activity ◽  
Catalytic Activity ◽  
Enzyme Immobilization ◽  
Mesoporous Materials ◽  
Specific Activity ◽  
Artificial Enzymes ◽  
Catalytic Nanoparticles ◽  
Solid Liquid ◽  
By Products ◽  
Insight Into

A new tendency toward the design of artificial enzymes based on nanostructures (nanodots, nanofibers, mesoporous materials) has emerged. On one hand, nanotechnology bestows self-catalytic nanoparticles with a specific activity to achieve efficient reactions with low number of by-products. On other hand, the nanoparticles may behave as nanometric scaffolds for hosting enzymes, promoting their catalytic activity and stability. In this case, enzyme immobilization requires the preservation of the catalytic activity by preventing enzyme unfolding and avoiding its aggregation. These approaches render many other advantages like hosting/storing enzymes in nanotechnological solid, liquid, and gel-like media. This chapter focuses on the most up-to-date approaches to manipulate or mimic enzyme activity based on nanotechnology, and offers examples of their applications in the most promising fields. It also gives new insight into the creation of reusable nanotechnological tools for enzyme storage.

scholarly journals Peptide-Mediated Immobilization on Magnetoferritin for Enzyme Recycling

Nanomaterials ◽  
2019 ◽  
Vol 9 (11) ◽  
pp. 1558 ◽  
Author(s):  
Zhang ◽  
Dong ◽  
Zhou ◽  
Hu ◽  
Li ◽  
...  
Keyword(s):  
Enzyme Immobilization ◽  
Immobilized Enzyme ◽  
Coiled Coil ◽  
Retention Rates ◽  
Generate Functional ◽  
Retention Efficiency ◽  
Enzyme Recycling ◽  
Lysine Residues ◽  
Target Molecules ◽  
Good Retention

Ferritin possess favorable properties because its exterior and interior surface can be applied to generate functional nanomaterials, which make them possible for enzyme immobilization and recycling. Here, we report the noncovalent immobilization of a genetically modified β-glucosidase onto the outer surface of synthetic magnetoferritin through the electrostatic interaction of a heterodimeric coiled-coil protein formed by coils containing lysine residues (K-coils) and coils containing glutamic acid (E-coils). The immobilized enzyme was characterized, and its enzymatic properties were evaluated. Furthermore, reusability of immobilized enzyme was demonstrated in aqueous solution under an applied magnetic field. The results showed that magnetoferritin was successfully prepared and it was an excellent support for enzyme immobilization. After three times usages, the retention rates were 93.75%, 82.5%, and 56.25%, respectively, demonstrating that immobilized enzyme possessed good retention efficiency and could be used as potential carrier for other biomolecules. The strategy of enzyme immobilization developed in this work can be applied, in general, to many other target molecules.

Continuous flow biodiesel production from wet microalgae using a hybrid thin film microfluidic platform

2018 ◽  
Vol 54 (85) ◽  
pp. 12085-12088 ◽  
Author(s):  
Eko K. Sitepu ◽  
Darryl B. Jones ◽  
Youhong Tang ◽  
Sophie C. Leterme ◽  
Kirsten Heimann ◽  
...  
Keyword(s):  
Thin Film ◽  
Continuous Flow ◽  
Room Temperature ◽  
Biodiesel Production ◽  
High Yield ◽  
Hybrid Thin Film ◽  
Microfluidic Platform ◽  
Thin Film Device ◽  
Wet Microalgae

A novel continuous flow turbo-thin film device (T2FD) is effective in producing biodiesel in high yield from wet microalgae at room temperature.

scholarly journals Optimization of enzyme immobilization on magnetic microparticles using 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide (EDC) as a crosslinking agent

2015 ◽  
Vol 7 (24) ◽  
pp. 10291-10298 ◽  
Author(s):  
F. Kazenwadel ◽  
H. Wagner ◽  
B. E. Rapp ◽  
M. Franzreb
Keyword(s):  
Enzyme Activity ◽  
Enzyme Immobilization ◽  
Crosslinking Agent ◽  
Magnetic Microparticles ◽  
Versatile Tool

Enzyme immobilization is a versatile tool in biotransformation processes to enhance enzyme activity and to secure an easy separation of catalysts and products and the reusability of enzymes.

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