Construction of portable electrochemical immunosensors based on graphene hydrogel@polydopamine for microcystin-LR detection using multi-mesoporous carbon sphere-enzyme labels

RSC Advances ◽  
2016 ◽  
Vol 6 (57) ◽  
pp. 51662-51669 ◽  
Author(s):  
Cuifen Gan ◽  
Zihong Sun ◽  
Li Ling ◽  
Zuyu He ◽  
Hongtao Lei ◽  
...  

A portable electrochemical immunosensor was fabricated for the detection of microcystin-LR by using graphene hydrogel@polydopamine as the substrate material and multi-HRP-(MCSs/Thi@AuNPs)-Ab2 as the signal label.

2020 ◽  
Vol 28 ◽  
Author(s):  
Hayati Filik ◽  
Asiye Aslıhan Avan ◽  
Mustafa Özyürek

: The prostate-specific antigen (PSA) has been considered a crucial serological marker for distinguishing prostate based cancer. This surveys recent progress in the construction of nanomaterial-based electrochemical immunosensors for a PSA. This review (from 2015 to 2020) reports the latest progress in PSA sensing based on the employ of different types of nanostructured materials. The most popular used nanostructured materials are metal, metal oxide, carbon-based nanomaterials, and their hybrid architectures utilized for distinct amplification protocols. In this review, the electrochemical immunosensors for prostate-specific antigen sensing are classified into three categories such as sandwich type@labeled, label free@nonlabeled and aptamer-based electrochemical immunosensor.


Chemosensors ◽  
2021 ◽  
Vol 9 (4) ◽  
pp. 85
Author(s):  
Anton Popov ◽  
Benediktas Brasiunas ◽  
Asta Kausaite-Minkstimiene ◽  
Almira Ramanaviciene

With the increasing importance of healthcare and clinical diagnosis, as well as the growing demand for highly sensitive analytical instruments, immunosensors have received considerable attention. In this review, electrochemical immunosensor signal amplification strategies using metal nanoparticles (MNPs) and quantum dots (Qdots) as tags are overviewed, focusing on recent developments in the ultrasensitive detection of biomarkers. MNPs and Qdots can be used separately or in combination with other nanostructures, while performing the function of nanocarriers, electroactive labels, or catalysts. Thus, different functions of MNPs and Qdots as well as recent advances in electrochemical signal amplification are discussed. Additionally, the methods most often used for antibody immobilization on nanoparticles, immunoassay formats, and electrochemical methods for indirect biomarker detection are overviewed.


2019 ◽  
Vol 54 (21) ◽  
pp. 13674-13684 ◽  
Author(s):  
Zidengya Jiang ◽  
Xin Xi ◽  
Shi Qiu ◽  
Dongqing Wu ◽  
Wei Tang ◽  
...  

Nanomaterials ◽  
2019 ◽  
Vol 9 (12) ◽  
pp. 1745 ◽  
Author(s):  
Alfredo Sánchez ◽  
Anabel Villalonga ◽  
Gonzalo Martínez-García ◽  
Concepción Parrado ◽  
Reynaldo Villalonga

Electrochemical immunosensors are antibody-based affinity biosensors with a high impact on clinical, environmental, food, and pharmaceutical analysis. In general, the analytical performance of these devices is critically determined by the materials and reagents used for their construction, signal production and amplification. Dendrimers are monodisperse and highly branched polymers with three-dimensional structures widely employed as “soft” nanomaterials in electrochemical immunosensor technology. This review provides an overview on the state-of-the-art in dendrimer-based electrochemical immunosensors, focusing on those using polyamidoamine and poly (propylene imine) dendrimers. Special emphasis is given to the most original methods recently reported for the construction of immunosensor architectures incorporating dendrimers, as well as to novel sensing approaches based on dendrimer-assisted signal enhancement strategies.


2010 ◽  
Vol 25 (5) ◽  
pp. 1130-1136 ◽  
Author(s):  
Xiaojun Chen ◽  
Kui Zhang ◽  
Jinjun Zhou ◽  
Jie Xuan ◽  
Wei Yan ◽  
...  

2019 ◽  
Vol 37 (4) ◽  
pp. 585-589
Author(s):  
Shuiping Li ◽  
Huajun Zhu ◽  
Guilong Xu ◽  
Qing Lin ◽  
Chengshuang Wang ◽  
...  

AbstractA three-step method was used for the synthesis of mesoporous carbon sphere (MCS) material: firstly, silica (SiO2) nanoparticles were synthesized by the modified Stöber procedure; secondly, tetraethyl orthosilicate (TEOS) and cetyltrimethylammonium chloride (CTAC) were grafted onto SiO2 core nanoparticles to prepare SiO2@SiO2-CTAC hard templates; lastly, MCS material was fabricated by carbonizing and selective leaching SiO2/resorcinol-formaldehyde (RF) composites. The influence of the mass ratio of R-F/silica on the structure, morphology and crystal form was studied. The results indicate that the MCS materials have a uniform morphology. The increase of the mass ratio of R-F/silica can increase the specific surface area and pore volume. The three-step method provides a cost-effective procedure for the fabrication of MCS materials with uniform morphology.


Small ◽  
2020 ◽  
Vol 16 (41) ◽  
pp. 2003943 ◽  
Author(s):  
Wanfeng Xiong ◽  
Hongfang Li ◽  
Huimin Wang ◽  
Jundong Yi ◽  
Hanhui You ◽  
...  

2018 ◽  
Vol 9 (1) ◽  
pp. 55-62
Author(s):  
Xiaolong Yu ◽  
Bogang Li

Mesoporous C@FexSy composite as a negative electrode for supercapacitors was synthesized via a one-step hydrothermal treatment followed by an electrodeposition process and its electrochemical properties were studied. Compared with bare carbon sphere, the electrochemical performance of C@FexSy composite was significantly improved, with a high specific capacitance (267.45 F/g), good rate performance (201.08 F/g at 2.5 A/g), and superior cycling stability (almost no capacitance degradation after 1000 cycles). The results show that the obtained C@FexSy composite is a promising negative electrode material for supercapacitors


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