Microbial assisted industrially important multiple enzymes from fish processing waste: purification, characterization and application for the simultaneous hydrolysis of lipid and protein molecules

RSC Advances ◽  
2016 ◽  
Vol 6 (96) ◽  
pp. 93602-93620 ◽  
Author(s):  
S. Hepziba Suganthi ◽  
K. Ramani

Fish processing waste (FPW) was evaluated as the substrate for the concomitant production of industrially important alkaline lipase and protease byStreptomyces thermolineatusfor the hydrolysis of lipid and protein rich FPW.

2009 ◽  
Vol 112 (1) ◽  
pp. 125-130 ◽  
Author(s):  
Talita S. Espósito ◽  
Ian P.G. Amaral ◽  
Diego S. Buarque ◽  
Givanildo B. Oliveira ◽  
Luiz B. Carvalho ◽  
...  

1970 ◽  
Vol 46 (3) ◽  
pp. 375-378 ◽  
Author(s):  
MM Islam ◽  
SM Masum ◽  
MM Rahman ◽  
AA Shaikh

The present investigation described the effective preparation of glucosamine hydrochloride (GluHCl) from chitin which was extracted from indigenous shrimp shell. GluHCl has attracted much attention owing to its therapeutic activity in osteoarthritis and widely used dietary supplement. The key step involved was extraction of chitin from shrimp skeleton and then hydrolysis of chitin by concentrated hydrochloric acid. The reaction proceeds via break down of glycoside linkage. Structural analysis was carried out by melting point, TLC, FT-IR, elemental analysis and all the data were compared with that of standard GluHCl. The elemental (C, 32.75; H, 6.51; N, 6.20) analysis is good concord with the calculated value (C, 33.42; H, 6.54; N, 6.50). Absence of v max at 1726 cm-1 indicates that GluHCl is a deacetylated product of chitin. The yields of the product mainly depend on reaction conditions. Maximum yield (63.5%) was obtained when chitin was hydrolyzed with concentrated HCl for 1.30 h. Key words: Shrimp shell; Chitin, Acid hydrolysis; Glucosamine hydrochloride Osteoarthritis. DOI: http://dx.doi.org/10.3329/bjsir.v46i3.9046 BJSIR 2011; 46(3): 375-378


Author(s):  
Tracey Anne Colley ◽  
Judith Valerian ◽  
Michael Zwicky Hauschild ◽  
Stig Irving Olsen ◽  
Morten Birkved

Nutrient depletion in Tanzanian sisal production has led to yield decreases over time. We use nutrient mass balances embedded within a life cycle assessment to quantify the extent of nutrient depletion for different production systems, then used circular economy principles to identify potential cosubstrates from within the Tanzanian economy to anaerobically digest with sisal wastes. The biogas produced is then used to generate bioelectricity and the digestate residual can be used as a fertilizer to address the nutrient depletion. If no current beneficial use of the cosubstrate was assumed, then beef manure and marine fish processing waste were the best cosubstrates. If agricultural wastes were assumed to have a current beneficial use as fertilizer, then marine fish processing waste and human urine were the best cosubstrates. The largest reduction in environmental impacts resulted from bioelectricity replacing electricity from fossil fuels in the national electricity grid and improved onsite waste management practices. There is significant potential to revitalize Tanzanian sisal production by applying circular economy principles to sisal waste management and bioenergy production.


Author(s):  
Benedykt Władyka ◽  
Katarzyna Pustelny

AbstractProteases, also referred to as peptidases, are the enzymes that catalyse the hydrolysis of peptide bonds in polipeptides. A variety of biological functions and processes depend on their activity. Regardless of the organism’s complexity, peptidases are essential at every stage of life of every individual cell, since all protein molecules produced must be proteolytically processed and eventually recycled. Protease inhibitors play a crucial role in the required strict and multilevel control of the activity of proteases involved in processes conditioning both the physiological and pathophysiological functioning of an organism, as well as in host-pathogen interactions. This review describes the regulation of activity of bacterial proteases produced by dangerous human pathogens, focusing on the Staphylococcus genus.


2020 ◽  
Vol 1 (1) ◽  
pp. 100-110 ◽  
Author(s):  
Taner Sar ◽  
Jorge A. Ferreira ◽  
Mohammad J. Taherzadeh

Abstract Fish processing towards production of fillet gives rise to wastewater streams that are ultimately directed to biogas production and/or wastewater treatment. However, these wastewater streams are rich in minerals, fat, and proteins that can be converted to protein-rich feed ingredients through submerged cultivation of edible filamentous fungi. In this study, the origin of wastewater stream, initial pH, cultivation time, and extent of washing during sieving, were found to influence the amount of recovered material from the wastewater streams and its protein content, following cultivation with Aspergillus oryzae. Through cultivation of the filamentous fungus in sludge, 330 kg of material per ton of COD were recovered by sieving, corresponding to 121 kg protein per ton of COD, while through its cultivation in salt brine, 210 kg of material were recovered per ton of COD, corresponding to 128 kg protein per ton of COD. Removal ranges of 12–43%, 39–92%, and 32–66% for COD, total solids, and nitrogen, respectively, were obtained after A. oryzae growth and harvesting in the wastewater streams. Therefore, the present study shows the versatility that the integration of fungal cultivation provides to fish processing industries, and should be complemented by economic, environmental, and feeding studies, in order to reveal the most promising valorization strategy. Graphic abstract


2018 ◽  
Vol 24 (1) ◽  
pp. 565
Author(s):  
Jajat Sudrajat ◽  
Komariyati Komariyati ◽  
Supriyanto Supriyanto

AbstrakUpaya ini merupakan integrasi antara usaha penanganan limbah pengolahan ikan menjadi pakan ternak dan upaya peningkatan gizi serta pendapatan keluarga kelompok usaha pengolah ikan di Desa Sungai Kakap. Metode yang digunakan dalam pelaksanaan kegiatan adalah sosialisasi, pelatihan, pendampingan, serta monitoring dan evaluasi. Hasil kegiatan menunjukkan tim pelaksana berhasil membangkitkan semangat berwirausaha bagi ibu-ibu anggota kelompok usaha olahan ikan yang tergabung dalam Kelompok Lina Sederhana dan kelompok rumah tangga lainnya. Keberhasilan ditunjukkan berupa peningkatan omzet penjualan melalui pengelolaan sistim produksi dan pemasaran sekaligus menjadi model usaha ramah lingkungan. Kegiatan ini dapat menjadi model pembinaan bagi lembaga terkait dalam meningkatkan keuntungan usaha olahan ikan dan mewujudkan model rumah tangga pangan lestari.Kata kunci: limbah olahan ikan, pakan ternak, ternak itikAbstractThis effort is an integration between the handling of fish processing waste as animal feed and the effort to increase the household’s nutrition and income of fish processing business group in Sungai Kakap village. The methods is used in the implementation are socialization, training, mentoring, and monitoring and evaluation. The results showed that the team is successful in generating entrepreneurial spirit for the women of fish processing business group belonging to the Lina Sederhana group and other household groups. The success is shown by increasing sales turnover through the management of production and marketing systems as well as being an environmental friendly business model. This activity can be an empowerment model for government institutions in increasing the profit of fish processing business and realizing the model of sustainable food household.Keywords: fish processed waste, animal feed, ducks


Author(s):  
Nickolay Pavlovich Dyachenko ◽  
Eduard Pavlovich Dyachenko ◽  
Yuriy Aleksandrovich Maksimenko

The article presents the research of kinetics and intensity of convective-radiation foam drying of gelatin broth from ordinary fish processing wastes in the Astrakhan region. Approximating dependences of dry gelatin removal from the unit area of the working surface per unit time on the varied factors have been obtained. The rational modes of the process, in which the maximum specific removal of the dry product is achieved, have been determined. It has been found that using only radiation energy supply makes the process of foaming gelatin broth difficult due to the uncontrolled sharp increase of temperature of the product and, as a consequence, the subsequent melting and destruction of the foam. Putting radiation energy supply into the process of convective foam drying of the gelatinized broth under rational conditions increases the specific productivity of the process by three times.


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