scholarly journals A new H2S-specific near-infrared fluorescence-enhanced probe that can visualize the H2S level in colorectal cancer cells in mice

2017 ◽  
Vol 8 (4) ◽  
pp. 2776-2781 ◽  
Author(s):  
Kun Zhang ◽  
Jie Zhang ◽  
Zhen Xi ◽  
Lu-Yuan Li ◽  
Xiangxiang Gu ◽  
...  
Keyword(s):  
Colorectal Cancer ◽  
Cancer Cells ◽  
Real Time ◽  
Near Infrared ◽  
Near Infrared Fluorescence ◽  
Real Time Imaging ◽  
Highly Sensitive ◽  
Colorectal Cancer Cells

A highly sensitive H2S-specific near-infrared fluorescence-enhanced probe was developed for real-time imaging of endogenous H2S in colorectal cancer cells (HCT116 and HT29) in mice.

Verbascoside Attenuates Rac-1 and HIF-1α Signaling Cascade in Colorectal Cancer Cells

2019 ◽  
Vol 18 (15) ◽  
pp. 2149-2155
Author(s):  
Danial Seyfi ◽  
Seyed B. Behzad ◽  
Mohammad Nabiuni ◽  
Kazem Parivar ◽  
Mohammad Tahmaseb ◽  
...  
Keyword(s):  
Colorectal Cancer ◽  
Colon Cancer ◽  
Cancer Cells ◽  
Real Time ◽  
Real Time Pcr ◽  
Mrna Levels ◽  
Dependent Manner ◽  
Colon Cancer Cells ◽  
Ht29 Cells ◽  
Colorectal Cancer Cells

Objective: Metastasis phenotype is considered as the main challenge in colon cancer therapeutic methods. Furthermore, the side effects of conventional colorectal cancer treatment methods have attracted a lot of attention into natural ingredients. The aim of the study was to assess the molecular mechanism of verbascoside as natural bio-compound in human HT29 colon cancer cells. Methods: HT29 cells were cultured in RPMI-1640 medium containing 10% FBS and 1% penicillin/ streptomycin at 37°C and 5% CO2. HT-29 cells were treated with different concentrations of verbascoside (10, 20, 30, 40, 50, 70, 100 µg/ml) for 24 hours, then MTT assay was used to calculate 50% inhibitory concentration. The migration of the colon cancer cells was evaluated by scratch assay. To evaluate involved antiproliferative mechanism, Rac-1 (Ras-related C3 botulinum toxin substrate 1) and HIF-1α (hypoxia-inducible factor-1α) related gene expression were evaluated by Real Time PCR. Results: The results showed that verbascoside inhibited HT29 colon cancer cell proliferation dose-dependently and IC50 was evaluated as 50 μg/ml (***P<0.001). The results of wound healing assay demonstrated verbascoside decreased cell migration in a dose dependent manner. In the IC50 treated HT29 cells metastatic progression was significantly suppressed as **P<0.01. The results of Real Time PCR showed an attenuating effect of verbascoside on Rac-1, Zeb-1 (zinc finger E-box binding homeobox 1), Arp2 (Actin-Related Proteins), Pak1 (p21 (RAC1) activated kinase 1), VEGF (Vascular endothelial growth factor) and HIF-1α as Epithelial-Mesenchymal Transition markers. The down regulation of mRNA levels was Rac-1= 15.38, HIF-1 α = 16.66, Pak-1, Arp-2= 6.25, VEGF=24.39, Zeb-1=35.71 in HT29 cells treated with IC50 concentration of verbascoside. Conclusion: Colorectal cancer cells induce Rac-1 and HIF-1α overexpression which plays an important role in the activation and progression of cell motility, angiogenesis and metastasis. Overall results showed that verbascoside elucidated significant anti-metastatic and anti-invasion activities through suppression of Rac-1, HIF-1α, and Zeb-1 signaling pathway and it may be a suitable candidate to overwhelm colon cancer metastatic phenotype.

Highly sensitive and specific cytosensing of HT 29 colorectal cancer cells using folic acid functionalized-KCC-1 nanoparticles

2019 ◽  
Vol 132 ◽  
pp. 122-131 ◽  
Author(s):  
Jafar Soleymani ◽  
Mohammad Hasanzadeh ◽  
Mohammad Hossein Somi ◽  
Nasrin Shadjou ◽  
Abolghasem Jouyban
Keyword(s):  
Colorectal Cancer ◽  
Folic Acid ◽  
Cancer Cells ◽  
Highly Sensitive ◽  
Colorectal Cancer Cells ◽  
Ht 29

Detection and quantification of colorectal cancer cells in peripheral blood and bone marrow by Real time RT-PCR

2003 ◽  
Vol 124 (4) ◽  
pp. A606-A607
Author(s):  
Nikolaus Ansorge ◽  
Kerem Bulut ◽  
Peter Felderbauer ◽  
Jan-Michel Otte ◽  
Young-Ran Suh ◽  
...  
Keyword(s):  
Colorectal Cancer ◽  
Bone Marrow ◽  
Cancer Cells ◽  
Real Time ◽  
Peripheral Blood ◽  
Rt Pcr ◽  
Colorectal Cancer Cells ◽  
Detection And Quantification

Near-infrared fluorescence cholangiography with indocyanine green for biliary atresia. Real-time imaging during the Kasai procedure: a pilot study

2015 ◽  
Vol 31 (12) ◽  
pp. 1177-1182 ◽  
Author(s):  
Yutaka Hirayama ◽  
Yasushi Iinuma ◽  
Naoyuki Yokoyama ◽  
Tetsuya Otani ◽  
Daisuke Masui ◽  
...  
Keyword(s):  
Pilot Study ◽  
Indocyanine Green ◽  
Biliary Atresia ◽  
Real Time ◽  
Near Infrared ◽  
Near Infrared Fluorescence ◽  
Real Time Imaging ◽  
Kasai Procedure

scholarly journals Detection of colorectal cancer cells from feces using quantitative real-time RT-PCR for colorectal cancer diagnosis

2008 ◽  
Author(s):  
Yoshikatsu Koga ◽  
Masahiro Yasunaga ◽  
Yoshihiro Moriya ◽  
Takayuki Akasu ◽  
Shin Fujita ◽  
...  
Keyword(s):  
Colorectal Cancer ◽  
Cancer Cells ◽  
Real Time ◽  
Cancer Diagnosis ◽  
Rt Pcr ◽  
Colorectal Cancer Cells

Near-infrared cyanine-based sensor for Fe3+ with high sensitivity: its intracellular imaging application in colorectal cancer cells

RSC Advances ◽  
2016 ◽  
Vol 6 (103) ◽  
pp. 100759-100764 ◽  
Author(s):  
Mingming Zhu ◽  
Chuanxing Shi ◽  
Xitao Xu ◽  
Zhiqian Guo ◽  
Weihong Zhu
Keyword(s):  
Colorectal Cancer ◽  
Cancer Cells ◽  
Near Infrared ◽  
High Sensitivity ◽  
Intracellular Imaging ◽  
Imaging Application ◽  
Colorectal Cancer Cells

A cyanine-based probe with N-(2-hydroxyethyl) amide arms was designed for Fe3+ with a remarkable colorimetric and fluorometric response. It was successfully applied to the imaging of Fe3+ ions in colorectal cancer cells.

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