AIE opens new applications in super-resolution imaging

2016 ◽  
Vol 4 (48) ◽  
pp. 7761-7765 ◽  
Author(s):  
Jiong Zhou ◽  
Guocan Yu ◽  
Feihe Huang
Keyword(s):  
Recent Work ◽  
Super Resolution ◽  
New Class ◽  
Turn On ◽  
Resolution Imaging ◽  
Fluorescence Turn On ◽  
New Applications

In a recent work, Tang and co-workers reported a new class of photoactivatable fluorescence turn-on AIE-based bioprobes which can be used for super-resolution imaging of mitochondria. This result opens a new door in the field of super-resolution imaging for developing a novel AIE-based bioprobe with high on–off contrast and a fast photoswitching rate.

A Mitochondrion-Specific Photoactivatable Fluorescence Turn-On AIE-Based Bioprobe for Localization Super-Resolution Microscope

2016 ◽  
Vol 28 (25) ◽  
pp. 5064-5071 ◽  
Author(s):  
Xinggui Gu ◽  
Engui Zhao ◽  
Teng Zhao ◽  
Miaomiao Kang ◽  
Chen Gui ◽  
...  
Keyword(s):  
Super Resolution ◽  
Turn On ◽  
Fluorescence Turn On

scholarly journals Bromo- and thiomaleimides as a new class of thiol-mediated fluorescence ‘turn-on’ reagents

2014 ◽  
Vol 12 (4) ◽  
pp. 557-560 ◽  
Author(s):  
Judith Youziel ◽  
Ahmed R. Akhbar ◽  
Qadeer Aziz ◽  
Mark E. B. Smith ◽  
Stephen Caddick ◽  
...  
Keyword(s):  
New Class ◽  
Turn On ◽  
Highly Effective ◽  
Fluorescence Turn On

Bromo- and thiomaleimides are shown to serve as highly effective quenchers of a covalently attached fluorophore. Reactions with thiols that lead to removal of the maleimide conjugation, or detachment of the fluorophore from the maleimide, result in ‘turn-on’ of the fluorescence.

scholarly journals Visualization and Manipulation of Actin Cytoskeleton with Small-Molecular Probes

2020 ◽  
Author(s):  
Takeru Takagi ◽  
Tasuku Ueno ◽  
Keisuke Ikawa ◽  
Daisuke Asanuma ◽  
Yusuke Nomura ◽  
...  
Keyword(s):  
Actin Cytoskeleton ◽  
Green Light ◽  
Dynamic Network ◽  
Super Resolution ◽  
Molecular Probes ◽  
Actin Binding ◽  
Mechanical Forces ◽  
Cellular Functions ◽  
New Class ◽  
Resolution Imaging

Actin is a ubiquitous cytoskeletal protein, forming a dynamic network that generates mechanical forces in the cell. Here, in order to dissect the complex mechanisms of actin-related cellular functions, we introduce two powerful tools based on a new class of actin-binding small molecule: one enables visualization of the actin cytoskeleton, including super-resolution imaging, and the other enables highly specific green-light-controlled fragmentation of actin filaments, affording unprecedented control of the actin cytoskeleton and its force network in living cells.

scholarly journals Visualization and Manipulation of Actin Cytoskeleton with Small-Molecular Probes

2020 ◽  
Author(s):  
Takeru Takagi ◽  
Tasuku Ueno ◽  
Keisuke Ikawa ◽  
Daisuke Asanuma ◽  
Yusuke Nomura ◽  
...  
Keyword(s):  
Actin Cytoskeleton ◽  
Green Light ◽  
Dynamic Network ◽  
Super Resolution ◽  
Molecular Probes ◽  
Actin Binding ◽  
Mechanical Forces ◽  
Cellular Functions ◽  
New Class ◽  
Resolution Imaging

Actin is a ubiquitous cytoskeletal protein, forming a dynamic network that generates mechanical forces in the cell. Here, in order to dissect the complex mechanisms of actin-related cellular functions, we introduce two powerful tools based on a new class of actin-binding small molecule: one enables visualization of the actin cytoskeleton, including super-resolution imaging, and the other enables highly specific green-light-controlled fragmentation of actin filaments, affording unprecedented control of the actin cytoskeleton and its force network in living cells.

scholarly journals Super-Resolution Imaging by Dielectric Superlenses: TiO2 Metamaterial Superlens versus BaTiO3 Superlens

Photonics ◽  
2021 ◽  
Vol 8 (6) ◽  
pp. 222
Author(s):  
Rakesh Dhama ◽  
Bing Yan ◽  
Cristiano Palego ◽  
Zengbo Wang
Keyword(s):  
Super Resolution ◽  
Field Of View ◽  
Nano Particles ◽  
Imaging Systems ◽  
Label Free ◽  
Theoretical Simulation ◽  
New Class ◽  
Physical Shape ◽  
Resolution Imaging ◽  
First Time

All-dielectric superlens made from micro and nano particles has emerged as a simple yet effective solution to label-free, super-resolution imaging. High-index BaTiO3 Glass (BTG) microspheres are among the most widely used dielectric superlenses today but could potentially be replaced by a new class of TiO2 metamaterial (meta-TiO2) superlens made of TiO2 nanoparticles. In this work, we designed and fabricated TiO2 metamaterial superlens in full-sphere shape for the first time, which resembles BTG microsphere in terms of the physical shape, size, and effective refractive index. Super-resolution imaging performances were compared using the same sample, lighting, and imaging settings. The results show that TiO2 meta-superlens performs consistently better over BTG superlens in terms of imaging contrast, clarity, field of view, and resolution, which was further supported by theoretical simulation. This opens new possibilities in developing more powerful, robust, and reliable super-resolution lens and imaging systems.

scholarly journals Tumor Protein D54 defines a new class of intracellular transport vesicle

2018 ◽  
Author(s):  
Gabrielle Larocque ◽  
Penelope J. La-Borde ◽  
Nicholas I. Clarke ◽  
Nicholas J. Carter ◽  
Stephen J. Royle
Keyword(s):  
Membrane Trafficking ◽  
Intracellular Transport ◽  
Super Resolution ◽  
Molecular Heterogeneity ◽  
Rab Gtpases ◽  
New Class ◽  
Transport Vesicle ◽  
Membrane Compartment ◽  
Generic Class ◽  
Resolution Imaging

Transport of proteins and lipids from one membrane compartment to another is via intracellular vesicles. We investigated the function of Tumor Protein D54 (TPD54/TPD52L2), and found that TPD54 was involved in multiple membrane trafficking pathways: anterograde traffic, recycling and Golgi integrity. To understand how TPD54 controls these diverse functions, we used an inducible method to reroute TPD54 to mitochondria. Surprisingly, this manipulation resulted in the capture of many small vesicles (30 nm diameter) at the mitochondrial surface. Super-resolution imaging confirmed the presence of similarly sized TPD54-positive structures under normal conditions. It appears that TPD54 defines a new class of transport vesicle, which we term intracellular nanovesicles (INVs). INVs meet three criteria for functionality. They contain specific cargo, they have certain R-SNAREs for fusion, and they are endowed with a variety of Rab GTPases (16 out of 43 tested). The molecular heterogeneity of INVs and the diverse functions of TPD54 suggest that INVs have various membrane origins and a number of destinations. We propose that INVs are a generic class of transport vesicle which transfer cargo between these varied locations.

Controlled optical characteristics of lanthanide doped upconversion nanoparticles for emerging applications

2017 ◽  
Vol 46 (48) ◽  
pp. 16729-16737 ◽  
Author(s):  
Xiaoqian Ge ◽  
Jinliang Liu ◽  
Lining Sun
Keyword(s):  
Super Resolution ◽  
Optical Characteristics ◽  
Upconversion Nanoparticles ◽  
Dye Sensitized ◽  
Recent Advances ◽  
Challenges And Opportunities ◽  
Resolution Imaging ◽  
New Applications

We highlight the recent advances of upconversion nanoparticles (UCNPs) in the field of emerging applications, such as dye sensitized UCNPs, photogene regulation, anti-counterfeiting, and super-resolution imaging. Finally, we discuss the challenges and opportunities in the development of these new applications.

A Boronic Acid-Based Fluorescence Hydrogel for Monosaccharide Detection

2018 ◽  
Author(s):  
Suying Xu ◽  
Adam Sedgwick ◽  
Souad Elfecky ◽  
Wenbo Chen ◽  
Ashley Jones ◽  
...  
Keyword(s):  
Fluorescent Probe ◽  
Boronic Acid ◽  
Binding Constants ◽  
Strong Fluorescence ◽  
Fluorescence Response ◽  
Turn On ◽  
Fluorescence Turn On ◽  
Hydrogel System

<p>A boronic acid-based anthracene fluorescent probe was functionalised with an acrylamide unit to incorporate into a hydrogel system for monosaccharide detection<i>. </i>In solution, the fluorescent probe<b> </b>displayed a strong fluorescence turn-on response upon exposure to fructose, and an expected trend in apparent binding constants, as judged by a fluorescence response where D-fructose > D-galactose > D-mannose > D-glucose. The hydrogel incorporating the boronic acid monomer demonstrated the ability to detect monosaccharides by fluorescence with the same overall trend as the monomer in solution with the addition of fructose resulting in a 10-fold enhancement (≤ 0.25 M). <b><u></u></b></p>

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