A study of biocatalysts based on glucose oxidase

2017 ◽  
Vol 202 ◽  
pp. 303-314 ◽  
Author(s):  
E. P. Golikova ◽  
N. V. Lakina ◽  
O. V. Grebennikova ◽  
V. G. Matveeva ◽  
E. M. Sulman
Keyword(s):  
Ftir Spectroscopy ◽  
Glucose Oxidase ◽  
Gluconic Acid ◽  
Oxidation Reaction ◽  
Immobilized Enzyme ◽  
Tight Binding ◽  
Crosslinking Agent ◽  
Covalent Immobilization ◽  
Modified Silica ◽  
Inorganic Support

During this work, we studied the possibility of glucose oxidase (GOx) covalent immobilization on a modified inorganic support. A series of GOx-based biocatalysts was synthesized by crosslinking the enzyme to a surface of modified silica or alumina. Polyelectrolyte layers were used as modifiers for the silica and alumina surfaces. These layers promote tight binding of the GOx to the support. The biocatalyst’s activity and stability were studied using an oxidation reaction of d-glucose to d-gluconic acid. It was found that GOx immobilized on the modified SiO2 using glutardialdehyde as a crosslinking agent was the most active and stable catalytic system, showing an 85% yield of gluconic acid. A study of the synthesized biocatalyst structure using FTIR spectroscopy showed that the enzyme was covalently crosslinked to the surface of an inorganic support modified with chitosan and glutardialdehyde. In the case of SiO2, the quantity of the immobilized enzyme was higher than in the case of Al2O3.

Simultaneous covalent immobilization of glucose oxidase and catalase onto chemically modified acrylonitrile copolymer membranes

2004 ◽  
Vol 91 (6) ◽  
pp. 4057-4063 ◽  
Author(s):  
T. Godjevargova ◽  
Rajeshwar Dayal ◽  
Ivaylo Marinov
Keyword(s):  
Glucose Oxidase ◽  
Covalent Immobilization ◽  
Acrylonitrile Copolymer ◽  
Chemically Modified

Use of Glucose Oxidase in a Membrane Reactor for Gluconic Acid Production

2007 ◽  
pp. 161-170 ◽  
Author(s):  
Luiz Carlos Martins das Neves ◽  
Michele Vitolo
Keyword(s):  
Glucose Oxidase ◽  
Gluconic Acid ◽  
Acid Production ◽  
Membrane Reactor ◽  
Gluconic Acid Production

Genome sequencing of Aureobasidium pullulans P25 and overexpression of a glucose oxidase gene for hyper-production of Ca2+-gluconic acid

2018 ◽  
Vol 112 (5) ◽  
pp. 669-678 ◽  
Author(s):  
Shou-Feng Zhao ◽  
Hong Jiang ◽  
Zhe Chi ◽  
Guang-Lei Liu ◽  
Zhen-Ming Chi ◽  
...  
Keyword(s):  
Glucose Oxidase ◽  
Genome Sequencing ◽  
Gluconic Acid ◽  
Aureobasidium Pullulans ◽  
Oxidase Gene ◽  
Glucose Oxidase Gene

scholarly journals Covalent Immobilization of Thiol Proteinases on Chitosan

2020 ◽  
Vol 2 (1) ◽  
pp. 7
Author(s):  
Svetlana Olshannikova ◽  
Victoria Koroleva ◽  
Marina Holyavka ◽  
Alexander Pashkov ◽  
Valeriy Artyukhov
Keyword(s):  
Specific Activity ◽  
Crosslinking Agent ◽  
Total Activity ◽  
Covalent Immobilization ◽  
Tropical Plants ◽  
Chitosan Matrix ◽  
Plant Enzymes ◽  
Thiol Proteases ◽  
Thiol Proteinases ◽  
Wide Substrate Specificity

Plant enzymes such as ficin (EC 3.4.22.3), papain (EC 3.4.22.2) and bromelain (EC 3.4.22.4) are obtained from tropical plants. These biocatalysts belong to thiol proteases, in the active center of which cysteine is contained. Ficin, papain and bromelain have a wide substrate specificity, which provides a demand for their use in various industries. Enzymes in the free state are less commonly used; immobilized biocatalysts are the preferred form. The aim of this work was to determine the optimal concentration of a crosslinking agent in the covalent immobilization of ficin, papain and bromelain on a chitosan matrix. Ficin, papain and bromelain (Sigma) were chosen as objects of study. An acid-soluble chitosan (350 kDa, Bioprogress CJSC) was used as an immobilization carrier. The concentration range of glutaraldehyde (crosslinking agent) ranged from 1 to 25%. Suitable concentrations of glutaraldehyde for covalent immobilization were identified by the optimal ratio of protein content (mg per g of carrier), total activity (in units per ml of solution) and specific activity (in units per mg of protein). It was shown that for covalent immobilization of ficin and bromelain on a chitosan matrix, it is most promising to use 10% glutaraldehyde. For immobilization of papain on chitosan by covalent means, the concentration of glutaraldehyde equal to 20% is optimal.

scholarly journals Antimicrobial activity of fifteen Italian honeys against Paenibacillus larvae ATCC 9545

2018 ◽  
Vol 68 (4) ◽  
pp. 547 ◽  
Author(s):  
S. SAGONA ◽  
B. TURCHI ◽  
F. FRATINI ◽  
M. GIUSTI ◽  
B. TORRACCA ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Glucose Oxidase ◽  
Inhibitory Activity ◽  
Oxidase Activity ◽  
Gluconic Acid ◽  
Total Flavonoid ◽  
Paenibacillus Larvae ◽  
Economic Losses ◽  
Total Phenolic ◽  
Honey Sample

Currently, American Foulbrood (AFB) represents one of the most important problems for beekeepers, due to economic losses and to the absence of an effective therapeutic treatment. The aim of this work was to characterize fifteen Italian honeys in order to assess their inhibitory activity against Paenibacillus larvae ATCC 9545. Each honey was analyzed for the activity of the following enzymes: glucose oxidase and catalase. Moreover, melissopalynological analysis and other biochemical parameters, namely gluconic acid, total phenolic and total flavonoid contents were determined. For each honey, the Minimum Inhibitory Concentration (M.I.C.) and the Minimum Bactericidal Concentration (M.B.C.) against P. larvae were determined. All tested honey samples had an inhibitory activity on P. larvae. In particular, the lowest M.I.C. and M.B.C. values (53.8 mg/mL and 107.5 mg/mL, respectively) were recorded for an Arbutus honey sample. Arbutus honeys also had the highest gluconic acid and total phenolic contents (12.6 ± 1.7 g/kg and 243.2 ± 25.1 mg/kg, respectively) and the highest glucose oxidase activity (13.0 ± 1.9 nM H2O2/min). Dark honeys, including Arbutus, seem to have a higher gluconic acid content and a higher antimicrobial activity. Thus, honey characterization, including colour and physico-chemical characteristics (e.g. gluconic acid concentration, total phenolic and total flavonoid contents, glucose oxidase activity), could be crucial for the assessment of its employment against P. larvae.

Oxidation of glucose to gluconic acid using a colloidal catalyst containing gold nanoparticles and glucose oxidase

2014 ◽  
Vol 63 (4) ◽  
pp. 1009-1016 ◽  
Author(s):  
N. A. Samoilova ◽  
M. A. Krayukhina ◽  
T. P. Klimova ◽  
T. A. Babushkina ◽  
O. V. Vyshivannaya ◽  
...  
Keyword(s):  
Gold Nanoparticles ◽  
Glucose Oxidase ◽  
Gluconic Acid ◽  
Oxidation Of Glucose

An ultrasensitive nanobiohybrid platform for glucose electrochemical biosensing based on ferrocenyl iminopropyl-modified silica nanoparticles

RSC Advances ◽  
2016 ◽  
Vol 6 (52) ◽  
pp. 46238-46243 ◽  
Author(s):  
M. Saadaoui ◽  
M. Braiek ◽  
N. Jaffrezic-Renault ◽  
N. Raouafi
Keyword(s):  
Bovine Serum Albumin ◽  
Serum Albumin ◽  
Silica Nanoparticles ◽  
Glucose Oxidase ◽  
Bovine Serum ◽  
Silica Nanoparticle ◽  
Modified Silica ◽  
Electrochemical Biosensing ◽  
Modified Silica Nanoparticles

A novel nanobiohybrid platform based on ferrocenyl iminopropyl-modified silica nanoparticle conjugates (fap-SiNPs), entrapped in glucose oxidase (GOx) and bovine serum albumin cross-linked with glutaraldehyde, was developed.

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