Inducible Bcl-2 gene RNA interference mediated by aptamer-integrated HDV ribozyme switch

2017 ◽  
Vol 9 (7) ◽  
pp. 619-626 ◽  
Author(s):  
Yuanyuan Zhang ◽  
Jine Wang ◽  
Hui Cheng ◽  
Na Sun ◽  
Min Liu ◽  
...  
Keyword(s):  
Rna Interference ◽  
Endogenous Gene ◽  
Hdv Ribozyme

We designed HDV ribozymes with an embedded theophylline aptamer and pri-miRNA of endogenous gene Bcl-2 to engineer an RNAi-regulatory device.

scholarly journals Developing Plantain for Resistance to Banana Aphids by RNA Interference

Proceedings ◽  
2020 ◽  
Vol 36 (1) ◽  
pp. 54
Author(s):  
Jekayinoluwa ◽  
Tripathi ◽  
Obiero ◽  
Muge ◽  
Dale ◽  
...  
Keyword(s):  
Rna Interference ◽  
Molecular Characterization ◽  
Smallholder Farmers ◽  
Insect Pest ◽  
Selective Medium ◽  
Temporary Immersion Bioreactor ◽  
Embryogenic Cell ◽  
Endogenous Gene ◽  
Culture Techniques ◽  
Transgenic Lines

Banana bunchy top virus (BBTV) is one of the world’s invasive species. Banana aphid (Pentalonia nigronervosa) is found in all banana producing areas and it is the insect pest known to transmit BBTV causing banana bunchy top disease (BBTD) in bananas and plantains (Musa spp.) and can cause a significant yield loss of up to 100% in severe cases. Controlling the spread of BBTD has been very challenging since there is no known endogenous gene in the Musa germplasm that could confer resistance to BBTV. Excessive dependence on insecticides for disease control is detrimental to the environment and off-target-organisms. The objective of this study was to use RNA interference (RNAi) targeting the acetylcholinesterase (AChE) gene in banana aphid to develop resistance against aphids in farmer preferred plantain cultivars. This could help sustain smallholder farmers in areas where BBTD is an epidemic. To achieve this, plantain cultivars were initiated using plant tissue culture techniques and rapidly multiplied using Temporary Immersion Bioreactor. This was followed by generation of embryogenic cell suspension (ECS), Agrobacterium-mediated transformation of banana and plantain ECS using a RNAi plasmid construct and molecular characterization of putative transgenic lines. Agro-infected ECS of banana and plantain cultivars were regenerated on selective medium and produced several transgenic lines. Molecular characterization confirmed the presence of transgene in about 80% transgenic lines. Preliminary glasshouse screening of transgenic lines showed reduction in population of banana aphids in comparison to control non-transgenic plants. This is the first report on using RNAi targeting AChE gene for developing transgenic plantain that are resistant to banana aphids.

scholarly journals RNA interference and its role in the regulation of eucaryotic gene expression.

2003 ◽  
Vol 50 (1) ◽  
pp. 217-229 ◽  
Author(s):  
Zofia Szweykowska-Kulińska ◽  
Artur Jarmołowski ◽  
Marek Figlerowicz
Keyword(s):  
Rna Interference ◽  
Germ Line ◽  
Rna Virus ◽  
Specific Gene ◽  
Double Stranded Rna ◽  
Endogenous Gene ◽  
Cellular Processes ◽  
Selective Degradation ◽  
Eukaryotic Organisms ◽  
Almost All

Several years ago it was discovered that plant transformation with a transcribed sense transgene could shut down the expression of a homologous endogenous gene. Moreover, it was shown that the introduction into the cell of dsRNA (double-stranded RNA) containing nucleotide sequence complementary to an mRNA sequence causes selective degradation of the latter and thus silencing of a specific gene. This phenomenon, called RNA interference (RNAi) was demonstrated to be present in almost all eukaryotic organisms. RNAi is also capable of silencing transposons in germ line cells and fighting RNA virus infection. Enzymes involved in this process exhibit high homology across species. Some of these enzymes are involved in other cellular processes, for instance developmental timing, suggesting strong interconnections between RNAi and other metabolic pathways. RNAi is probably an ancient mechanism that evolved to protect eukaryotic cells against invasive forms of nucleic acids.

scholarly journals H3K9me3 is Required for Inheritance of Small RNAs that Target a Unique Subset of Newly Evolved Genes

2018 ◽  
Author(s):  
Itamar Lev ◽  
Hila Gingold ◽  
Oded Rechavi
Keyword(s):  
Rna Interference ◽  
Caenorhabditis Elegans ◽  
Small Rnas ◽  
Foreign Gene ◽  
Small Interfering Rnas ◽  
Endogenous Gene ◽  
C Elegans ◽  
Histone 3 ◽  
Repressive Chromatin ◽  
Genome Wide

AbstractIn Caenorhabditis elegans, RNA interference (RNAi) responses can transmit across generations via small RNAs. RNAi inheritance is associated with Histone-3-Lysine-9 tri-methylation (H3K9me3) of the targeted genes. In other organisms, maintenance of silencing requires a feed-forward loop between H3K9me3 and small RNAs. Here we show that in C. elegans not only is H3K9me3 unnecessary for inheritance, the modification’s function depends on the identity of the RNAi-targeted gene. We found an asymmetry in the requirement for H3K9me3 and the main worm H3K9me3 methyltransferases, SET-25 and SET-32. Both methyltransferases promote heritable silencing of the foreign gene gfp, but are dispensable for silencing of the endogenous gene oma-1. Genome-wide examination of heritable endogenous small interfering RNAs (endo-siRNAs) revealed that the SET-25-dependent heritable endo-siRNAs target newly acquired and highly H3K9me3 marked genes. Thus, “repressive” chromatin marks could be important specifically for heritable silencing of genes which are flagged as “foreign”, such as gfp.

Using RNA interference to manipulate endogenous gene expression in Schistosoma mansoni sporocysts

2003 ◽  
Vol 128 (2) ◽  
pp. 205-215 ◽  
Author(s):  
Jon P Boyle ◽  
Xiao-Jun Wu ◽  
Chuck B Shoemaker ◽  
Timothy P Yoshino
Keyword(s):  
Gene Expression ◽  
Rna Interference ◽  
Schistosoma Mansoni ◽  
Endogenous Gene

Inhibiting primary effusion lymphoma by lentiviral vectors encoding short hairpin RNA

Blood ◽  
2005 ◽  
Vol 105 (6) ◽  
pp. 2510-2518 ◽  
Author(s):  
Andrew Godfrey ◽  
John Anderson ◽  
Antigoni Papanastasiou ◽  
Yasu Takeuchi ◽  
Chris Boshoff
Keyword(s):  
Rna Interference ◽  
Human Cancer ◽  
Primary Effusion Lymphoma ◽  
Kaposi Sarcoma ◽  
Lentiviral Vectors ◽  
Death Domain ◽  
Inhibitory Protein ◽  
Endogenous Gene

AbstractWe use lentiviral-delivered RNA interference (RNAi) to inhibit the growth of a model of primary effusion lymphoma (PEL) in vitro and in vivo. RNAi is a phenomenon allowing the sequence-specific targeting and silencing of exogenous and endogenous gene expression and is being applied to inhibit viral replication both in vitro and in vivo. We show that silencing of genes believed to be essential for the Kaposi sarcoma-associated herpesvirus (KSHV) latent life cycle (the oncogenic cluster) has a varied effect in PEL cell lines cultured in vitro, however, concomitant silencing of the viral cyclin (vcyclin) and viral FLICE (Fas-associating protein with death domain-like interleukin-1β-converting enzyme) inhibitory protein (vFLIP) caused efficient apoptosis in all PEL lines tested. We demonstrate that in a murine model of PEL, lentiviral-mediated RNA interference both inhibits development of ascites and can act as a treatment for established ascites. We also show that the administered lentiviral vectors are essentially limited to the peritoneal cavity, which has advantages for safety and dosage in a therapeutic setting. This shows the use of lentiviral-mediated RNA interference in vivo as a potential therapeutic against a virally driven human cancer.

Regulation of MAP4K4 gene expression by RNA interference through an engineered theophylline-dependent hepatitis delta virus ribozyme switch

2016 ◽  
Vol 12 (11) ◽  
pp. 3370-3376 ◽  
Author(s):  
Hui Cheng ◽  
Yuanyuan Zhang ◽  
Hongyan Wang ◽  
Na Sun ◽  
Min Liu ◽  
...  
Keyword(s):  
Gene Expression ◽  
Rna Interference ◽  
Hepatitis Delta Virus ◽  
Hepatitis Delta ◽  
New Approach ◽  
Effector Domain ◽  
Hdv Ribozyme ◽  
Gene Regulatory ◽  
Inducible Rnai ◽  
Delta Virus

We chose the HDV ribozyme with an embedded theophylline aptamer as the sensor domain and the pri-miRNA as the effector domain to engineer a gene-regulatory device, and developed a new approach for inducible RNAi in the control of MAP4K4 gene expression.

scholarly journals CRISPR/Cas9-Mediated Hitchhike Expression of Functional shRNAs at the Porcine miR-17-92 Cluster

Cells ◽  
2019 ◽  
Vol 8 (2) ◽  
pp. 113 ◽  
Author(s):  
Chao Lu ◽  
Daxin Pang ◽  
Mengjing Li ◽  
Hongming Yuan ◽  
Tingting Yu ◽  
...  
Keyword(s):  
Gene Expression ◽  
Gene Therapy ◽  
Rna Interference ◽  
Integration Site ◽  
Classical Swine Fever ◽  
Mirna Cluster ◽  
Transgenic Pigs ◽  
Blastocyst Development ◽  
Endogenous Gene ◽  
Fetal Fibroblasts

Successful RNAi applications depend on strategies allowing stable and persistent expression of minimal gene silencing triggers without perturbing endogenous gene expression. In this study, we proposed an endogenous microRNA (miRNA) cluster as a novel integration site for small hairpin RNAs (shRNAs). We successfully integrated exogenous shRNAs at the porcine miRNA-17-92 (pmiR-17-92) cluster via a CRISPR/Cas9-mediated knock-in strategy. The anti-EGFP or anti-CSFV shRNAs could be stably and effectively expressed at the control of the endogenous promoter of the pmiR-17-92 cluster. Importantly, we confirmed that hitchhike expression of anti- classical swine fever (CSFV) shRNA had no effect on cell growth, blastocyst development and endogenous pmiR-17-92 expression in selected transgene (TG) porcine fetal fibroblasts (PFFs) clones. Moreover, these TG PFFs could inhibit the replication of CSFV by half and could be further used for generation of transgenic pigs. Taken together, these results show that our RNA interference (RNAi) expression strategy benefits numerous applications, from miRNA, genome and transgenic research, to gene therapy.

Lentivirus-mediated RNA interference reveals that a testis-specific gene,LM23,is essential for spermatogenesis inRattus norvegicus

2010 ◽  
Vol 34 (8) ◽  
pp. S11-S11
Author(s):  
Mei‑ling Liu ◽  
Meng‑chun Jia
Keyword(s):  
Rna Interference ◽  
Specific Gene

RNA Interference Technology

2005 ◽  
Author(s):  
Andrew Fire ◽  
Marshall Nirenberg
Keyword(s):  
Rna Interference

The oncolytic adenovirus Ad-iREP-2 utilizes p53-dependent rna interference for tumor-selective replication

2008 ◽  
Vol 46 (09) ◽  
Author(s):  
E Gürlevik ◽  
P Schache ◽  
L Zender ◽  
MP Manns ◽  
S Kubicka ◽  
...  
Keyword(s):  
Rna Interference ◽  
Oncolytic Adenovirus ◽  
Tumor Selective
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