scholarly journals Countercurrent liquid–liquid extraction on paper

Lab on a Chip ◽  
2017 ◽  
Vol 17 (20) ◽  
pp. 3401-3404 ◽  
Author(s):  
Gert IJ. Salentijn ◽  
Maciej Grajewski ◽  
Elisabeth Verpoorte
Keyword(s):  
Liquid Extraction ◽  
Countercurrent Flow ◽  
Proof Of Concept ◽  
Two Phase ◽  
Liquid Liquid Extraction

We demonstrate the realization of two-phase countercurrent flow on paper and its use in a proof-of-concept liquid–liquid extraction.

Selection of a recyclable in situ liquid–liquid extraction solvent for foam-free synthesis of rhamnolipids in a two-phase fermentation

2020 ◽  
Vol 22 (23) ◽  
pp. 8495-8510 ◽  
Author(s):  
Philipp Demling ◽  
Maximilian von Campenhausen ◽  
Carolin Grütering ◽  
Till Tiso ◽  
Andreas Jupke ◽  
...  
Keyword(s):  
Liquid Extraction ◽  
Stirred Tank ◽  
Biotechnological Production ◽  
Two Phase ◽  
Stirred Tank Reactors ◽  
Extraction Solvent ◽  
Liquid Liquid Extraction ◽  
Extraction Solvents ◽  
Selection Of

A multi-step reductive approach for the selection of in situ extraction solvents for bioprocesses was developed enabling a foam-free biotechnological production of rhamnolipids in stirred-tank reactors.

scholarly journals Separation of digoxin by luiquid-luiquid extraction from extracts of foxglove secondary glycosides

2014 ◽  
Vol 68 (2) ◽  
pp. 161-170 ◽  
Author(s):  
Vesna Novkovic ◽  
Ljiljana Stanojevic ◽  
Milorad Cakic ◽  
Vlada Veljkovic ◽  
Mihajlo Stankovic
Keyword(s):  
Aqueous Ethanol ◽  
Volume Ratio ◽  
Liquid Extraction ◽  
Light Phase ◽  
Initial Amount ◽  
Two Phase ◽  
Liquid Liquid Extraction ◽  
Extraction Degree ◽  
Phase Systems ◽  
Extracting Solvent

The present study deals with the extraction of digoxin (Dgx) from chloroform and trichloroethylene extracts of the secondary glycosides of fermented foxglove (Digitalis lanata Ehrh.) foliage by liquid-liquid extraction. The extraction degree (ED) of Dgx achieved by maceration and percolation using 10% vol. aqueous ethanol solutions were higher than 95%. Using trichlorethylene and chloroform, the ED of Dgx of about 100% and 96%, respectively from the liquid ethanolic extracts (macerate or percolate) were achieved by the four-cycle extraction. Fifteen separating funnels were employed for the liquid-liquid extraction. Three different four-component two-phase systems (ethanol:water - chloroform:ethyl acetate, ethanol:water - chloroform:trichloroethylene and ethanol:water - trichloroethylene:ethyl acetate) were tested as an extracting solvent to get the final product having more than 98% of Dgx. The initial amount of the chloroform or trichloroethylene extract in the light phase was varied between 5 and 25 g/L, while the volume ratio of light and heavy phases was in the range of 1:1 to 1:2. The best Dgx yield of 98% was achieved with the system ethanol:water - chloroform:trichloroethylene 35:15:20:30 at the volume ratio of the phases of 1:1.1 and at the initial amount of the extract of 15 g/L. Purity of the separated digoxin was 99.8 %.

scholarly journals Stability of clavulanic acid in PEG/citrate and liquid–liquid extraction in aqueous two-phase system

2014 ◽  
Vol 375 ◽  
pp. 104-109 ◽  
Author(s):  
M.N. Carneiro-da-Cunha ◽  
K.P.S. Souza ◽  
A.M.O. Mota ◽  
J.A. Teixeira ◽  
C.S. Porto ◽  
...  
Keyword(s):  
Clavulanic Acid ◽  
Liquid Extraction ◽  
Phase System ◽  
Two Phase ◽  
Aqueous Two Phase System ◽  
Liquid Liquid Extraction

scholarly journals Preparative Separation of Alkaloids from Stem of Euchresta tubulosa Dunn. by High-Speed Counter-Current Chromatography Using Stepwise Elution

Molecules ◽  
2019 ◽  
Vol 24 (24) ◽  
pp. 4602 ◽  
Author(s):  
Weixin Li ◽  
Huan Wang ◽  
Aiwen Dong
Keyword(s):  
High Speed ◽  
Methylene Chloride ◽  
Liquid Extraction ◽  
Preparative Separation ◽  
Target Compound ◽  
Two Phase ◽  
Chinese Herbal ◽  
Liquid Liquid Extraction ◽  
Counter Current ◽  
First Time

Euchresta tubulosa Dunn. is a Chinese herbal medicine with biological activity, but there are few studies on its components at present. Alkaloids in the stem of Euchresta tubulosa Dunn. were isolated and purified by high-speed counter-current chromatography (HSCCC) using stepwise elution. First of all, liquid-liquid extraction (methylene chloride-methanol-water, 5:1:4, v/v) was used for the preliminary enrichment. According to the partition coefficient (K) of a target compound in a series of different two-phase solvents, the final result was that carbon tetrachloride-methylene chloride-methanol-water (2:3:3:2, v/v) (1) and methylene chloride-methanol-water (5:3:2, v/v) (2) were suitable for the HSCCC using stepwise elution. As a result, the purity was all higher than 93% and matrine (1), oxymatrine (2), N-formyl cytisine (3), and N-acetyl cytisine (4) can be eluted at one time by this mode. Cytisine-type alkaloids were isolated for the first time in this plant. Finally, the applicability of the mode was verified.

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