A semi-rigid isoindoline-derived nitroxide spin label for RNA

2018 ◽  
Vol 16 (5) ◽  
pp. 816-824 ◽  
Author(s):  
Dnyaneshwar B. Gophane ◽  
Burkhard Endeward ◽  
Thomas F. Prisner ◽  
Snorri Th. Sigurdsson
Keyword(s):  
Epr Spectroscopy ◽  
Spin Label ◽  
Spin Labels ◽  
Pulsed Epr ◽  
Rna Duplexes ◽  
Limited Motion

The new semi-rigid spin label ImUm showed limited motion in RNA duplexes and accurate distances between two spin labels in RNA duplexes were obtained by pulsed EPR spectroscopy.

scholarly journals A Spirocyclohexyl Nitroxide Amino Acid Spin Label for Pulsed EPR Spectroscopy Distance Measurements

2010 ◽  
Vol 16 (19) ◽  
pp. 5778-5782 ◽  
Author(s):  
Andrzej Rajca ◽  
Velavan Kathirvelu ◽  
Sandip K. Roy ◽  
Maren Pink ◽  
Suchada Rajca ◽  
...  
Keyword(s):  
Amino Acid ◽  
Epr Spectroscopy ◽  
Spin Label ◽  
Distance Measurements ◽  
Pulsed Epr ◽  
Nitroxide Amino Acid

Amplitude of Pancreatic Lipase Lid Opening in Solution and Identification of Spin Label Conformational Subensembles by Combining Continuous Wave and Pulsed EPR Spectroscopy and Molecular Dynamics

Biochemistry ◽  
2010 ◽  
Vol 49 (10) ◽  
pp. 2140-2149 ◽  
Author(s):  
Sebastien Ranaldi ◽  
Valérie Belle ◽  
Mireille Woudstra ◽  
Raphael Bourgeas ◽  
Bruno Guigliarelli ◽  
...  
Keyword(s):  
Molecular Dynamics ◽  
Pancreatic Lipase ◽  
Epr Spectroscopy ◽  
Spin Label ◽  
Continuous Wave ◽  
Pulsed Epr

Long-range distance determinations in biomacromolecules by EPR spectroscopy

2007 ◽  
Vol 40 (1) ◽  
pp. 1-53 ◽  
Author(s):  
Olav Schiemann ◽  
Thomas F. Prisner
Keyword(s):  
Long Range ◽  
Epr Spectroscopy ◽  
Structural Changes ◽  
Continuous Wave ◽  
Double Resonance ◽  
Theoretical Background ◽  
Spin Labels ◽  
Paramagnetic Resonance ◽  
Pulsed Epr ◽  
Amino Acid Radicals

AbstractElectron paramagnetic resonance (EPR) spectroscopy provides a variety of tools to study structures and structural changes of large biomolecules or complexes thereof. In order to unravel secondary structure elements, domain arrangements or complex formation, continuous wave and pulsed EPR methods capable of measuring the magnetic dipole coupling between two unpaired electrons can be used to obtain long-range distance constraints on the nanometer scale. Such methods yield reliably and precisely distances of up to 80 Å, can be applied to biomolecules in aqueous buffer solutions or membranes, and are not size limited. They can be applied either at cryogenic or physiological temperatures and down to amounts of a few nanomoles. Spin centers may be metal ions, metal clusters, cofactor radicals, amino acid radicals, or spin labels. In this review, we discuss the advantages and limitations of the different EPR spectroscopic methods, briefly describe their theoretical background, and summarize important biological applications. The main focus of this article will be on pulsed EPR methods like pulsed electron–electron double resonance (PELDOR) and their applications to spin-labeled biosystems.

Phase memory relaxation times of spin labels in human carbonic anhydrase II: pulsed EPR to determine spin label location

2001 ◽  
Vol 94 (3) ◽  
pp. 245-256 ◽  
Author(s):  
M Huber ◽  
M Lindgren ◽  
P Hammarström ◽  
L.-G Mårtensson ◽  
U Carlsson ◽  
...  
Keyword(s):  
Carbonic Anhydrase ◽  
Spin Label ◽  
Relaxation Times ◽  
Carbonic Anhydrase Ii ◽  
Spin Labels ◽  
Pulsed Epr ◽  
Phase Memory ◽  
Human Carbonic Anhydrase

scholarly journals A novel strategy for site selective spin-labeling to investigate bioactive entities by DNP and EPR spectroscopy

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Kevin Herr ◽  
Max Fleckenstein ◽  
Martin Brodrecht ◽  
Mark V. Höfler ◽  
Henrike Heise ◽  
...  
Keyword(s):  
Solid State ◽  
Epr Spectroscopy ◽  
Spin Label ◽  
Tertiary Structure ◽  
Spin Labeling ◽  
Disulfide Bridge ◽  
Bioactive Molecules ◽  
Spin Labels ◽  
Time Saving ◽  
Site Selective

AbstractA novel specific spin-labeling strategy for bioactive molecules is presented for eptifibatide (integrilin) an antiplatelet aggregation inhibitor, which derives from the venom of certain rattlesnakes. By specifically labeling the disulfide bridge this molecule becomes accessible for analytical techniques such as Electron Paramagnetic Resonance (EPR) and solid state Dynamic Nuclear Polarization (DNP). The necessary spin-label was synthesized and inserted into the disulfide bridge of eptifibatide via reductive followed by insertion by a double Michael addition under physiological conditions. This procedure is universally applicable for disulfide containing biomolecules and is expected to preserve their tertiary structure with minimal change due to the small size of the label and restoring of the previous disulfide connection. HPLC and MS analysis show the successful introduction of the spin label and EPR spectroscopy confirms its activity. DNP-enhanced solid state NMR experiments show signal enhancement factors of up to 19 in 13C CP MAS experiments which corresponds to time saving factors of up to 361. This clearly shows the high potential of our new spin labeling strategy for the introduction of site selective radical spin labels into biomolecules and biosolids without compromising its conformational integrity for structural investigations employing solid-state DNP or advanced EPR techniques.

Spin Label Study of Apomembranes and Purple Membranes

1993 ◽  
Vol 48 (5-6) ◽  
pp. 500-503
Author(s):  
Tzvetana R. Lazarova ◽  
Maya Y. Velitchkova
Keyword(s):  
Fatty Acids ◽  
Lipid Bilayer ◽  
Spin Label ◽  
Hyperfine Splitting ◽  
Esr Spectra ◽  
Spin Labels ◽  
Label Study ◽  
Splitting Parameter ◽  
Induced Changes ◽  
Purple Membranes

Abstract Three spin-labelled fatty acids were used to detect the dynamics of lipid bilayer of apomem branes and purple membranes. It was found that ESR spectra of spin labels bound to apo­ membranes showed a temperature-induced changes rather similar to those seen with purple membranes. At the same time, however, the values of hyperfine splitting parameter 2Tm were lower as compared to purple membranes. The results pointed out that the removal of the retinal from purple membranes affects the dynamics of lipid bilayer and apomembranes were more rigid structure than those of purple membranes.

Characterization of Bifunctional Spin Labels for Investigating the Structural and Dynamic Properties of Membrane Proteins Using EPR Spectroscopy

2017 ◽  
Vol 121 (39) ◽  
pp. 9185-9195 ◽  
Author(s):  
Indra D. Sahu ◽  
Andrew F. Craig ◽  
Megan M. Dunagum ◽  
Robert M. McCarrick ◽  
Gary A. Lorigan
Keyword(s):  
Membrane Proteins ◽  
Epr Spectroscopy ◽  
Dynamic Properties ◽  
Spin Labels

scholarly journals Aminoxyl Radicals of B/P Frustrated Lewis Pairs: Refinement of the Spin-Hamiltonian Parameters by Field- and Temperature-Dependent Pulsed EPR Spectroscopy

PLoS ONE ◽  
2016 ◽  
Vol 11 (6) ◽  
pp. e0157944 ◽  
Author(s):  
Marcos de Oliveira ◽  
Robert Knitsch ◽  
Muhammad Sajid ◽  
Annika Stute ◽  
Lisa-Maria Elmer ◽  
...  
Keyword(s):  
Epr Spectroscopy ◽  
Spin Hamiltonian ◽  
Frustrated Lewis Pairs ◽  
Temperature Dependent ◽  
Pulsed Epr ◽  
Hamiltonian Parameters ◽  
Aminoxyl Radicals ◽  
Lewis Pairs
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