scholarly journals Efficient cross-effect dynamic nuclear polarization without depolarization in high-resolution MAS NMR

2017 ◽  
Vol 8 (12) ◽  
pp. 8150-8163 ◽  
Author(s):  
Frédéric Mentink-Vigier ◽  
Guinevere Mathies ◽  
Yangping Liu ◽  
Anne-Laure Barra ◽  
Marc A. Caporini ◽  
...  
Keyword(s):  
High Resolution ◽  
Dynamic Nuclear Polarization ◽  
Nuclear Polarization ◽  
Mas Nmr ◽  
Cross Effect ◽  
Nmr Sensitivity

The mixed trityl-TEMPO biradical TEMTriPol-1 provides excellent MAS NMR sensitivity with DNP while avoiding nuclear depolarization.

Up to 100% Improvement in Dynamic Nuclear Polarization Solid-State NMR Sensitivity Enhancement of Polymers by Removing Oxygen

2015 ◽  
Vol 36 (15) ◽  
pp. 1416-1421 ◽  
Author(s):  
Dao Le ◽  
Fabio Ziarelli ◽  
Trang N. T. Phan ◽  
Giulia Mollica ◽  
Pierre Thureau ◽  
...  
Keyword(s):  
Solid State ◽  
Dynamic Nuclear Polarization ◽  
Solid State Nmr ◽  
Nuclear Polarization ◽  
Sensitivity Enhancement ◽  
Nmr Sensitivity

scholarly journals Dynamic Nuclear Polarization Enhanced MAS NMR Spectroscopy for Structural Analysis of HIV-1 Protein Assemblies

2016 ◽  
Vol 120 (2) ◽  
pp. 329-339 ◽  
Author(s):  
Rupal Gupta ◽  
Manman Lu ◽  
Guangjin Hou ◽  
Marc A. Caporini ◽  
Melanie Rosay ◽  
...  
Keyword(s):  
Nmr Spectroscopy ◽  
Structural Analysis ◽  
Dynamic Nuclear Polarization ◽  
Nuclear Polarization ◽  
Mas Nmr ◽  
Protein Assemblies ◽  
Hiv 1

scholarly journals Atomic-Scale Structure of Mesoporous Silica-Encapsulated Pt and PtSn Nanoparticles Revealed by Dynamic Nuclear Polarization-Enhanced 29Si MAS NMR Spectroscopy

2019 ◽  
Vol 123 (12) ◽  
pp. 7299-7307 ◽  
Author(s):  
Evan Wenbo Zhao ◽  
Raghu Maligal-Ganesh ◽  
Frederic Mentink-Vigier ◽  
Tommy Yunpu Zhao ◽  
Yong Du ◽  
...  
Keyword(s):  
Nmr Spectroscopy ◽  
Mesoporous Silica ◽  
Dynamic Nuclear Polarization ◽  
Nuclear Polarization ◽  
Scale Structure ◽  
Mas Nmr ◽  
Atomic Scale ◽  
29Si Mas Nmr ◽  
Atomic Scale Structure ◽  
Ptsn Nanoparticles

Temperature dependence of cross-effect dynamic nuclear polarization in rotating solids: advantages of elevated temperatures

2016 ◽  
Vol 18 (44) ◽  
pp. 30696-30704 ◽  
Author(s):  
Michel-Andreas Geiger ◽  
Marcella Orwick-Rydmark ◽  
Katharina Märker ◽  
W. Trent Franks ◽  
Dmitry Akhmetzyanov ◽  
...  
Keyword(s):  
Temperature Dependence ◽  
Dynamic Nuclear Polarization ◽  
Nuclear Polarization ◽  
Elevated Temperatures ◽  
Cross Effect

DNP on proteins at 200 K.

scholarly journals In Situ Characterization of Pharmaceutical Formulations by Dynamic Nuclear Polarization Enhanced MAS NMR

2017 ◽  
Vol 121 (34) ◽  
pp. 8132-8141 ◽  
Author(s):  
Qing Zhe Ni ◽  
Fengyuan Yang ◽  
Thach V. Can ◽  
Ivan V. Sergeyev ◽  
Suzanne M. D’Addio ◽  
...  
Keyword(s):  
Dynamic Nuclear Polarization ◽  
Nuclear Polarization ◽  
Pharmaceutical Formulations ◽  
Mas Nmr ◽  
In Situ Characterization

Static 1H dynamic nuclear polarization with the biradical TOTAPOL: a transition between the solid effect and the cross effect

2014 ◽  
Vol 16 (14) ◽  
pp. 6687-6699 ◽  
Author(s):  
Daphna Shimon ◽  
Akiva Feintuch ◽  
Daniella Goldfarb ◽  
Shimon Vega
Keyword(s):  
High Temperatures ◽  
Dynamic Nuclear Polarization ◽  
Low Temperatures ◽  
Nuclear Polarization ◽  
Cross Effect ◽  
Solid Effect ◽  
The Cross

Static 1H-DNP with TOTAPOL: the solid effect (SE) dominant at low temperatures; the cross effect (CE) dominant at high temperatures; and DNP-buildup: Tbu(SE) < Tbu(CE).

High-resolution proton and laser photochemically induced dynamic nuclear polarization NMR studies of cation binding to bovine .alpha.-lactalbumin

Biochemistry ◽  
1987 ◽  
Vol 26 (18) ◽  
pp. 5769-5774 ◽  
Author(s):  
Lawrence J. Berliner ◽  
Keiko Koga ◽  
Hiroyasu Nishikawa ◽  
Julie E. Scheffler
Keyword(s):  
High Resolution ◽  
Dynamic Nuclear Polarization ◽  
Nuclear Polarization ◽  
Cation Binding ◽  
Nmr Studies ◽  
Alpha Lactalbumin

scholarly journals Competing Transfer Pathways in Direct and Indirect Dynamic Nuclear Polarization MAS NMR Experiments on HIV-1 Capsid Assemblies: Implications for Sensitivity and Resolution

2021 ◽  
Author(s):  
Ivan V. Sergeyev ◽  
Caitlin M. Quinn ◽  
Jochem Struppe ◽  
Angela Gronenborn ◽  
Tatyana Polenova
Keyword(s):  
Dynamic Nuclear Polarization ◽  
Nuclear Polarization ◽  
Magic Angle Spinning ◽  
Optimal Choice ◽  
Mas Nmr ◽  
Magic Angle ◽  
Large Signal ◽  
Experimental Conditions ◽  
Natural Isotopic Abundance ◽  
Hiv 1

Abstract. Dynamic nuclear polarization-enhanced (DNP) magic angle spinning (MAS) NMR of biological systems is a rapidly growing field. Large signal enhancements make the technique particularly attractive for signal-limited cases, such as studies of complex biological assemblies or at natural isotopic abundance. However, spectral resolution is considerably reduced compared to ambient-temperature non-DNP spectra. Herein, we report a systematic investigation into sensitivity and resolution of 1D and 2D 13C-detected DNP MAS NMR experiments on HIV-1 CA tubular assemblies. We show that the magnitude and sign of signal enhancement as well as the homogeneous line width are strongly dependent on the biradical concentration, the dominant polarization transfer pathway, and the enhancement buildup time. Our findings provide guidance for optimal choice of sample preparation and experimental conditions in DNP experiments.

scholarly journals Maximizing NMR signal per unit time by facilitating the e–e–n cross effect DNP rate

2018 ◽  
Vol 20 (43) ◽  
pp. 27646-27657 ◽  
Author(s):  
Alisa Leavesley ◽  
Sheetal Jain ◽  
Ilia Kamniker ◽  
Hui Zhang ◽  
Suchada Rajca ◽  
...  
Keyword(s):  
Spin Relaxation ◽  
Electron Spin ◽  
Dynamic Nuclear Polarization ◽  
Nuclear Spin ◽  
Nuclear Polarization ◽  
Nuclear Spin Relaxation ◽  
Cross Effect ◽  
Spin Clusters

Dynamic nuclear polarization (DNP) efficiency is critically dependent on the properties of the radical, solvent, and solute, where electron spin clusters will shorten nuclear spin relaxation and enhance CE-DNP.

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