Rapid and sensitive detection of viral nucleic acids using silicon microchips

The Analyst ◽  
2018 ◽  
Vol 143 (11) ◽  
pp. 2596-2603 ◽  
Author(s):  
Laura Powell ◽  
Rodrigo Sergio Wiederkehr ◽  
Paige Damascus ◽  
Maarten Fauvart ◽  
Federico Buja ◽  
...  
Keyword(s):  
Nucleic Acids ◽  
Sensitive Detection ◽  
Technology Platform ◽  
Silicon Microchip

Rapid and sensitive amplification of viral nucleic acids is feasible on a flexible silicon microchip technology platform.

Gold nanorods-based lateral flow biosensors for sensitive detection of nucleic acids

2021 ◽  
Vol 188 (4) ◽  
Author(s):  
Qingcai Yu ◽  
Jing Zhang ◽  
Wanwei Qiu ◽  
Kun Li ◽  
Lisheng Qian ◽  
...  
Keyword(s):  
Nucleic Acids ◽  
Gold Nanorods ◽  
Lateral Flow ◽  
Sensitive Detection

Isothermal Self-Primer EXPonential Amplification Reaction (SPEXPAR) for Highly Sensitive Detection of Single-Stranded Nucleic Acids and Proteins

2021 ◽  
Author(s):  
Jun Chen ◽  
Daozhong Zhu ◽  
Ting Huang ◽  
Zizhong Yang ◽  
Birong Liu ◽  
...  
Keyword(s):  
Nucleic Acids ◽  
Sensitive Detection ◽  
Highly Sensitive ◽  
Highly Sensitive Detection ◽  
Amplification Reaction

Highly sensitive and rapid detection of citrus Huanglongbing pathogen (Candidatus Liberibacter asiaticus) using Cas12a-based methods

2021 ◽  
Author(s):  
Matthew Wheatley ◽  
Yong-Ping Duan ◽  
Yinong Yang
Keyword(s):  
Early Detection ◽  
Nucleic Acids ◽  
Detection Sensitivity ◽  
Sensitive Detection ◽  
Detection Methods ◽  
Candidatus Liberibacter Asiaticus ◽  
Fluorescent Reporter ◽  
Candidatus Liberibacter ◽  
Citrus Huanglongbing ◽  
Liberibacter Asiaticus

Citrus Huanglongbing (HLB) or greening is one of the most devastating diseases of citrus worldwide. Sensitive detection of its causal agent, Candidatus Liberibacter asiaticus (CLas), is critical for early diagnosis and successful management of HLB. However, current nucleic acid-based detection methods are often insufficient for the early detection of CLas from asymptomatic tissue, and unsuitable for high-throughput and field-deployable diagnosis of HLB. Here we report the development of the Cas12a-based DETECTR (DNA endonuclease-targeted CRISPR trans reporter) assay for highly specific and sensitive detection of CLas nucleic acids from infected samples. The DETECTR assay, which targets the five-copy nrdB gene specific to CLas, couples isothermal amplification with Cas12a trans-cleavage of fluorescent reporter oligos and enables detection of CLas nucleic acids at the attomolar level. The DETECTR assay was capable of specifically detecting the presence of CLas across different infected citrus, periwinkle and psyllid samples, and shown to be compatible with lateral flow assay technology for potential field-deployable diagnosis. The improvements in detection sensitivity and flexibility of the DETECTR technology position the assay as a potentially suitable tool for early detection of CLas in infected regions.

Dual-target one-step nested PCR for sensitive detection of SARS-CoV-2 nucleic acids

2021 ◽  
pp. 1-7
Author(s):  
Qijie Li ◽  
Yiqing Xia ◽  
Dunshui Liao ◽  
Hu Nie ◽  
Ming Zhang ◽  
...  
Keyword(s):  
Nucleic Acids ◽  
Nested Pcr ◽  
Sensitive Detection ◽  
One Step ◽  
Dual Target

Rapid and sensitive detection of “Candidatus Liberibacter asiaticus” by cycleave isothermal and chimeric primer-initiated amplification of nucleic acids

2008 ◽  
Vol 74 (2) ◽  
pp. 151-155 ◽  
Author(s):  
Naoya Urasaki ◽  
Shinji Kawano ◽  
Hiroyuki Mukai ◽  
Takashi Uemori ◽  
Osamu Takeda ◽  
...  
Keyword(s):  
Nucleic Acids ◽  
Sensitive Detection ◽  
Candidatus Liberibacter Asiaticus ◽  
Candidatus Liberibacter ◽  
Liberibacter Asiaticus

Sensitive detection of microRNA by chronocoulometry and rolling circle amplification on a gold electrode

2014 ◽  
Vol 50 (68) ◽  
pp. 9704-9706 ◽  
Author(s):  
Bo Yao ◽  
Yichen Liu ◽  
Miyuki Tabata ◽  
Huangtianzhi Zhu ◽  
Yuji Miyahara
Keyword(s):  
Nucleic Acids ◽  
Gold Electrode ◽  
Rolling Circle Amplification ◽  
Sensitive Detection ◽  
Quantitative Detection ◽  
Rolling Circle

An integrated gold electrode fabricated on a polystyrene substrate was developed for quantitative detection of nucleic acids by rolling circle amplification and chronocoulometry.

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