Chromatographic fingerprint based on serially coupled columns combined with multiple-component quantitation with a single reference standard for quality evaluation of Shen–Zhi–Ling oral liquid

Abstract Rat bioassay was used to assess the protein quality of powdered infant formulas and to evaluate the feasibility of using modified casein diets (containing the same source and level of fat and carbohydrate contributed by the infant formulas) as reference standards. Modification of the casein diet to match the milk-based formulas caused a significant reduction in weekly protein efficiency ratios (PER) and net protein ratios (NPR) at the third and fourth weeks. Modification of the casein diet to simulate the soy-based formulas had no significant effect on NPR values; PER values were more varied. When PER and NPR values of the powdered milk-based formulas were expressed relative to the unmodified reference standard, the relative values were lower than when each matched reference was used. With few exceptions, the relative weekly PER values of the soy-based formulas were similar regardless of the standard used. The relative NPR values of the formulas had a pattern similar to the relative PER values. The data indicate that protein quality evaluation of infant formulas using rat bioassay warrants the use of matched casein reference diets for each type of formula.

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Sensitivity Analysis of the Quality Evaluation System for Baikal Skullcap Root

Applied Mechanics and Materials ◽

10.4028/www.scientific.net/amm.40-41.212 ◽

2010 ◽

Vol 40-41 ◽

pp. 212-219

Author(s):

Ke Li ◽

Wei Cheng ◽

Xiao Jian Liu ◽

Shu Bin Li ◽

En Guang Hou ◽

...

Keyword(s):

Sensitivity Analysis ◽

Evaluation System ◽

Quality Evaluation ◽

Evaluation Model ◽

Chromatographic Fingerprint ◽

Traditional Chinese Herbal Medicine ◽

Chinese Herbal ◽

Sensitivity Indices ◽

Quality Evaluation System ◽

Quality Evaluation Model

In this paper, with the aid of a quality evaluation model, we study sensitivity analysis of the quality evaluation system for Baikal skullcap root and compute related sensitivity indices, hence determine the response of pharmacological effect under the model by varying chromatographic fingerprint data. This result is new and provides us with a feasible method for the quality evaluation of Traditional Chinese Herbal Medicine.

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Optimisation of gradient elution with serially-coupled columns Part II: Multi-linear gradients

Journal of Chromatography A ◽

10.1016/j.chroma.2014.10.100 ◽

2014 ◽

Vol 1373 ◽

pp. 51-60 ◽

Cited By ~ 9

Author(s):

C. Ortiz-Bolsico ◽

J.R. Torres-Lapasió ◽

M.C. García-Alvarez-Coque

Keyword(s):

Gradient Elution ◽

Coupled Columns ◽

Serially Coupled Columns

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Development of the chromatographic fingerprint of herbal preparations Shuang–Huang–Lian oral liquid

Journal of Pharmaceutical and Biomedical Analysis ◽

10.1016/j.jpba.2006.01.060 ◽

2006 ◽

Vol 41 (3) ◽

pp. 845-856 ◽

Cited By ~ 59

Author(s):

Yuhua Cao ◽

Licong Wang ◽

Xuejun Yu ◽

Jiannong Ye

Keyword(s):

Chromatographic Fingerprint ◽

Herbal Preparations ◽

Oral Liquid

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Chromatographic fingerprinting and quantitative analysis for the quality evaluation of Xinfeng capsule

Acta Chromatographica ◽

10.1556/1326.2020.00743 ◽

2020 ◽

pp. 1-7

Author(s):

Leilei Gao ◽

Fang Wang ◽

Mei Meng

Keyword(s):

Quantitative Analysis ◽

High Performance ◽

Quality Evaluation ◽

Hierarchical Cluster ◽

Array Detector ◽

Diode Array Detector ◽

Chromatographic Fingerprint ◽

Xinfeng Capsule ◽

Single Marker ◽

The Common

Abstract A simple, accurate and sensitive method of high performance liquid chromatography (HPLC) with diode array detector was established to identify Xinfeng capsules and systematically evaluated its quality, based on chromatographic fingerprint integrated with the similarity analysis, hierarchical cluster analysis and the quantitative analysis of multi-components by single marker (QAMS). In this study, 18 peaks were selected as the common peaks to evaluate the similarities among different batches (S1–S10) of Xinfeng capsules samples, which were manufactured in the First Affiliated Hospital of Anhui University of Chinese Medicine with a three-year span. Compared to control fingerprint, the similarities values for 10 batches of samples were more than 0.90. Moreover, by analyzing the reference of astragalus, the chromatogram of astragalus was developed, and 10 common peaks of astragalus were identified. More importantly, simultaneous quantification of three markers in Xinfeng capsule, including Calycosin-7-glucoside, calycosin and Formononetinaldehyde was performed, the three constituents showed good regression (R > 0.999) within linear ranges, and their recoveries were within the range of 97.6–101.5%. The validation results showed that the developed method was specific, accurate, precise and robust. This study demonstrated that the developed method offers an efficient, reliable and practical approach for systematic quality evaluation of Xinfeng capsule.

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