Organic semiconducting nanoprobe with redox-activatable NIR-II fluorescence forin vivoreal-time monitoring of drug toxicity

2019 ◽  
Vol 55 (1) ◽  
pp. 27-30 ◽  
Author(s):  
Yufu Tang ◽  
Yuanyuan Li ◽  
Zhen Wang ◽  
Feng Pei ◽  
Xiaoming Hu ◽  
...  
Keyword(s):  
Nitric Oxide ◽  
Real Time ◽  
Drug Toxicity ◽  
Drug Dose ◽  
Non Invasive ◽  
Dose Dependent

A nitric-oxide-activatable organic semiconducting nanoprobe was developed forin vivo,in situ, real-time and non-invasive NIR-II fluorescence monitoring of drug-dose-dependent hepatotoxicity.

Hybrid MPI-MRI System for Dual-Modal In Situ Cardiovascular Assessments of Real-Time 3D Blood Flow Quantification—A Pre-Clinical In Vivo Feasibility Investigation

2020 ◽  
Vol 39 (12) ◽  
pp. 4335-4345
Author(s):  
Jochen Franke ◽  
Nicoleta Baxan ◽  
Heinrich Lehr ◽  
Ulrich Heinen ◽  
Sebastian Reinartz ◽  
...  
Keyword(s):  
Blood Flow ◽  
Real Time ◽  
Flow Quantification ◽  
Blood Flow Quantification

scholarly journals Non-invasive, real-time reporting drug release in vitro and in vivo

2015 ◽  
Vol 51 (32) ◽  
pp. 6948-6951 ◽  
Author(s):  
Yanfeng Zhang ◽  
Qian Yin ◽  
Jonathan Yen ◽  
Joanne Li ◽  
Hanze Ying ◽  
...  
Keyword(s):  
Drug Release ◽  
Real Time ◽  
Near Infrared ◽  
Reporting System ◽  
Real Time Monitoring ◽  
Near Infrared Fluorescence ◽  
Non Invasive ◽  
Fluorescence Dye

Anin vitroandin vivodrug-reporting system is developed for real-time monitoring of drug release via the analysis of the concurrently released near-infrared fluorescence dye.

scholarly journals Non-invasive continuous real-time in vivo analysis of microbial hydrogen production shows adaptation to fermentable carbohydrates in mice

2018 ◽  
Vol 8 (1) ◽  
Author(s):  
José M. S. Fernández-Calleja ◽  
Prokopis Konstanti ◽  
Hans J. M. Swarts ◽  
Lianne M. S. Bouwman ◽  
Vicenta Garcia-Campayo ◽  
...  
Keyword(s):  
Hydrogen Production ◽  
Real Time ◽  
Non Invasive ◽  
In Vivo Analysis ◽  
Fermentable Carbohydrates

Real-time in vivo optical biopsy using confocal laser endomicroscopy to evaluate distal margin in situ and determine surgical procedure in low rectal cancer

2018 ◽  
Vol 33 (7) ◽  
pp. 2332-2338
Author(s):  
Zhangyuanzhu Liu ◽  
Xiaobei Luo ◽  
Wei Jiang ◽  
Dexin Chen ◽  
Weisheng Chen ◽  
...  
Keyword(s):  
Rectal Cancer ◽  
Real Time ◽  
Surgical Procedure ◽  
Low Rectal Cancer ◽  
Optical Biopsy ◽  
Confocal Laser Endomicroscopy ◽  
Confocal Laser ◽  
Distal Margin

Real time non-invasive imaging of receptor-ligand interactions in vivo

2003 ◽  
Vol 90 (3) ◽  
pp. 454-463 ◽  
Author(s):  
Paul Winnard, ◽  
Venu Raman
Keyword(s):  
Real Time ◽  
Receptor Ligand ◽  
Non Invasive ◽  
Ligand Interactions

scholarly journals Phasic contractions of rat mesenteric lymphatics increase basal and phasic nitric oxide generation in vivo

2009 ◽  
Vol 297 (4) ◽  
pp. H1319-H1328 ◽  
Author(s):  
H. Glenn Bohlen ◽  
Wei Wang ◽  
Anatoliy Gashev ◽  
Olga Gasheva ◽  
Dave Zawieja
Keyword(s):  
Nitric Oxide ◽  
Relaxation Mechanism ◽  
Lymph Flow ◽  
Lymphatic Endothelium ◽  
Contraction Frequency ◽  
Diastolic Relaxation ◽  
Intravenous Saline ◽  
First Time

Multiple investigators have shown interdependence of lymphatic contractions on nitric oxide (NO) activity by pharmacological and traumatic suppression of endothelial NO synthase (eNOS). We demonstrated that lymphatic diastolic relaxation is particularly sensitive to NO from the lymphatic endothelium. The predicted mechanism is shear forces produced by the lymph flow during phasic pumping, activating eNOS in the lymphatic endothelium to produce NO. We measured [NO] during phasic contractions using microelectrodes on in situ mesenteric lymphatics in anesthetized rats under basal conditions and with an intravenous saline bolus (0.5 ml/100 g) or infusion (0.5 ml·100 g−1·h−1). Under basal conditions, [NO] measured on the tubular portions of the lymphatics was ∼200–250 nM, slightly higher than in the adjacent adipocyte microvasculature, whereas [NO] measured on the lymphatic bulb surface was ∼400 nM. Immunohistochemistry of eNOS in isolated lympathics indicated a much greater expression in the lymph valves and surrounding bulb area than in the tubular regions. During phasic lymphatic contractions, the valve and tubular [NO] increased with each contraction, and during intravenous saline infusion, [NO] increased in proportion to the contraction frequency and, presumably, lymph flow. The partial blockade of eNOS over ∼1 cm length with Nω-nitro-l-arginine methyl ester lowered the [NO]. These in vivo data document for the first time that both valvular and tubular lymphatic segments increase NO generation during each phasic contraction and that [NO] summated with increased contraction frequency. The combined data predict regional variations in eNOS and [NO] in the tubular and valve areas, plus the summated NO responses dependent on contraction frequency provide for a complex relaxation mechanism involving NO.

Analytical strategies for real-time, non-invasive tracking of carbon nanomaterials in vivo

2013 ◽  
Vol 48 ◽  
pp. 1-13 ◽  
Author(s):  
Aijuan Liu ◽  
Shumei Zhai ◽  
Bin Zhang ◽  
Bing Yan
Keyword(s):  
Real Time ◽  
Carbon Nanomaterials ◽  
Non Invasive ◽  
Analytical Strategies

Determination of mosquito bloodmeal pH in situ by ion-selective microelectrode measurement: implications for the regulation of malarial gametogenesis

Parasitology ◽  
2000 ◽  
Vol 120 (6) ◽  
pp. 547-551 ◽  
Author(s):  
O. BILLKER ◽  
A. J. MILLER ◽  
R. E. SINDEN
Keyword(s):  
Xanthurenic Acid ◽  
Mosquito Vector ◽  
Dependent Manner ◽  
Ph Increase ◽  
Ph Range ◽  
Dose Dependent

Malarial gametocytes circulate in the peripheral blood of the vertebrate host as developmentally arrested intra-erythrocytic cells, which only resume development into gametes when ingested into the bloodmeal of the female mosquito vector. The ensuing development encompasses sexual reproduction and mediates parasite transmission to the insect. In vitro the induction of gametogenesis requires a drop in temperature and either a pH increase from physiological blood pH (ca pH 7·4) to about pH 8·0, or the presence of a gametocyte-activating factor recently identified as xanthurenic acid (XA). However, it is unclear whether either the pH increase or XA act as natural triggers in the mosquito bloodmeal. We here use pH-sensitive microelectrodes to determine bloodmeal pH in intact mosquitoes. Measurements taken in the first 30 min after ingestion, when malarial gametogenesis is induced in vivo, revealed small pH increases from 7·40 (mouse blood) to 7·52 in Aedes aegypti and to 7·58 in Anophěles stephensi. However, bloodmeal pH was clearly suboptimal if compared to values required to induce gametogenesis in vitro. Xanthurenic acid is shown to extend the pH-range of exflagellation in vitro in a dose-dependent manner to values that we have observed in the bloodmeal, suggesting that in vivo malarial gametogenesis could be further regulated by both these factors.

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