Real time non-invasive imaging of receptor-ligand interactions in vivo

2003 ◽  
Vol 90 (3) ◽  
pp. 454-463 ◽  
Author(s):  
Paul Winnard, ◽  
Venu Raman
Keyword(s):  
Real Time ◽  
Receptor Ligand ◽  
Non Invasive ◽  
Ligand Interactions

scholarly journals Non-invasive, real-time reporting drug release in vitro and in vivo

2015 ◽  
Vol 51 (32) ◽  
pp. 6948-6951 ◽  
Author(s):  
Yanfeng Zhang ◽  
Qian Yin ◽  
Jonathan Yen ◽  
Joanne Li ◽  
Hanze Ying ◽  
...  
Keyword(s):  
Drug Release ◽  
Real Time ◽  
Near Infrared ◽  
Reporting System ◽  
Real Time Monitoring ◽  
Near Infrared Fluorescence ◽  
Non Invasive ◽  
Fluorescence Dye

Anin vitroandin vivodrug-reporting system is developed for real-time monitoring of drug release via the analysis of the concurrently released near-infrared fluorescence dye.

scholarly journals Non-invasive continuous real-time in vivo analysis of microbial hydrogen production shows adaptation to fermentable carbohydrates in mice

2018 ◽  
Vol 8 (1) ◽  
Author(s):  
José M. S. Fernández-Calleja ◽  
Prokopis Konstanti ◽  
Hans J. M. Swarts ◽  
Lianne M. S. Bouwman ◽  
Vicenta Garcia-Campayo ◽  
...  
Keyword(s):  
Hydrogen Production ◽  
Real Time ◽  
Non Invasive ◽  
In Vivo Analysis ◽  
Fermentable Carbohydrates

scholarly journals Multiplexed Non-invasive in vivo Imaging to Assess Metabolism and Receptor Engagement in Tumor Xenografts

2019 ◽  
Author(s):  
Alena Rudkouskaya ◽  
Nattawut Sinsuebphon ◽  
Marien Ochoa ◽  
Joe E. Mazurkiewicz ◽  
Xavier Intes ◽  
...  
Keyword(s):  
Drug Delivery ◽  
Targeted Delivery ◽  
Near Infrared ◽  
Metabolic Response ◽  
Imaging Modalities ◽  
Wide Field ◽  
Receptor Ligand ◽  
Non Invasive ◽  
Intracellular Drug Delivery

AbstractFollowing an ever-increased focus on personalized medicine, there is a continuing need to develop preclinical molecular imaging modalities to guide the development and optimization of targeted therapies. To date, non-invasive quantitative imaging modalities that can comprehensively assess simultaneous cellular drug delivery efficacy and therapeutic response are lacking. In this regard, Near-Infrared (NIR) Macroscopic Fluorescence Lifetime Förster Resonance Energy Transfer (MFLI-FRET) imaging offers a unique method to robustly quantify receptor-ligand engagement in vivo and subsequent intracellular internalization, which is critical to assess the delivery efficacy of targeted therapeutics. However, implementation of multiplexing optical imaging with FRET in vivo is challenging to achieve due to spectral crowding and cross-contamination. Herein, we report on a strategy that relies on a dark quencher that enables simultaneous assessment of receptor-ligand engagement and tumor metabolism in intact live mice. First, we establish that IRDye QC-1 (QC-1) is an effective NIR dark acceptor for the FRET-induced quenching of donor Alexa Fluor 700 (AF700) using in vitro NIR FLI microscopy and in vivo wide-field MFLI imaging. Second, we report on simultaneous in vivo imaging of the metabolic probe IRDye 800CW 2-deoxyglucose (2-DG) and MFLI-FRET imaging of NIR-labeled transferrin FRET pair (Tf-AF700/Tf-QC-1) uptake in tumors. Such multiplexed imaging revealed an inverse relationship between 2-DG uptake and Tf intracellular delivery, suggesting that 2-DG signal may predict the efficacy of intracellular targeted delivery. Overall, our methodology enables for the first time simultaneous non-invasive monitoring of intracellular drug delivery and metabolic response in preclinical studies.

Analytical strategies for real-time, non-invasive tracking of carbon nanomaterials in vivo

2013 ◽  
Vol 48 ◽  
pp. 1-13 ◽  
Author(s):  
Aijuan Liu ◽  
Shumei Zhai ◽  
Bin Zhang ◽  
Bing Yan
Keyword(s):  
Real Time ◽  
Carbon Nanomaterials ◽  
Non Invasive ◽  
Analytical Strategies

Functional biomedical hydrogels for in vivo imaging

2016 ◽  
Vol 4 (48) ◽  
pp. 7793-7812 ◽  
Author(s):  
Kewen Lei ◽  
Qian Ma ◽  
Lin Yu ◽  
Jiandong Ding
Keyword(s):  
Real Time ◽  
In Vivo Imaging ◽  
Structure And Function ◽  
Non Invasive ◽  
The Status ◽  
And Function

In vivo imaging of biomedical hydrogels enables real-time and non-invasive visualization of the status of structure and function of hydrogels.

scholarly journals Point-of-Care Pathology with Miniature Microscopes

2011 ◽  
Vol 34 (3) ◽  
pp. 81-98 ◽  
Author(s):  
Jonathan T. C. Liu ◽  
Nathan O. Loewke ◽  
Michael J. Mandella ◽  
Richard M. Levenson ◽  
James M. Crawford ◽  
...  
Keyword(s):  
Real Time ◽  
New Technologies ◽  
Point Of Care ◽  
Definitive Treatment ◽  
Clinical Tool ◽  
Training Requirements ◽  
Non Invasive ◽  
Complex Process ◽  
Molecular Indicators

Advances in optical designs are enabling the development of miniature microscopes that can examine tissue in situ for early anatomic and molecular indicators of disease, in real time, and at cellular resolution. These new devices will lead to major changes in how diseases are detected and managed, driving a shift from today's diagnostic paradigm of biopsy followed by histopathology and recommended therapy, to non-invasive point-of-care diagnosis with possible same-session definitive treatment. This shift may have major implications for the training requirements of future physicians to enable them to interpret real-timein vivomicroscopic data, and will also shape the emerging fields of telepathology and telemedicine. Implementation of new technologies into clinical practice is a complex process that requires bridging gaps between clinicians, engineers and scientists. This article provides a forward-looking discussion of these issues, with a focus on malignant and pre-malignant lesions, by first highlighting some of the clinical areas where point-of-carein vivomicroscopy could address unmet needs, and then by reviewing the technological challenges that are being addressed, or need to be addressed, forin vivomicroscopy to become a standard clinical tool.

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