A novel colorimetric and far-red emission ratiometric fluorescent probe for the highly selective and ultrafast detection of hypochlorite in water and its application in bioimaging

The Analyst ◽  
2020 ◽  
Vol 145 (3) ◽  
pp. 939-945 ◽  
Author(s):  
Yuanyuan Zhang ◽  
Yufan Ma ◽  
Zhuo Wang ◽  
Xueyan Zhang ◽  
Xin Chen ◽  
...  
Keyword(s):  
Reactive Oxygen Species ◽  
Fluorescent Probe ◽  
Hypochlorous Acid ◽  
Reactive Oxygen ◽  
Oxygen Species ◽  
Red Emission ◽  
Physiological Processes ◽  
Ultrafast Detection ◽  
Ratiometric Fluorescent Probe

Hypochlorous acid (HOCl)/hypochlorite (OCl−), an important reactive oxygen species, plays a number of important roles in various physiological processes.

scholarly journals Development of an ultrafast fluorescent probe for specific recognition of hypochlorous acid and its application in live cells

RSC Advances ◽  
2021 ◽  
Vol 11 (40) ◽  
pp. 24669-24672
Author(s):  
Zhencai Xu ◽  
Xiaofeng Wang ◽  
Tingting Duan ◽  
Rong He ◽  
Fangwu Wang ◽  
...  
Keyword(s):  
Reactive Oxygen Species ◽  
Fluorescent Probe ◽  
Hypochlorous Acid ◽  
Reactive Oxygen ◽  
Inflammatory Cells ◽  
Live Cells ◽  
Oxygen Species ◽  
Specific Recognition

Hypochlorous acid (HOCl), a highly potent oxidant of reactive oxygen species, plays critical roles in many physiological and pathological processes. In this work, we designed a simple coumarin-based fluorescent probe, Cou–HOCl, for detecting HOCl in inflammatory cells.

A two-photon fluorescent probe for ratiometric imaging of endogenous hypochlorous acid in live cells and tissues

2017 ◽  
Vol 53 (78) ◽  
pp. 10800-10803 ◽  
Author(s):  
Yong Woong Jun ◽  
Sourav Sarkar ◽  
Subhankar Singha ◽  
Ye Jin Reo ◽  
Hye Rim Kim ◽  
...  
Keyword(s):  
Reactive Oxygen Species ◽  
Fluorescent Probe ◽  
Hypochlorous Acid ◽  
Reactive Oxygen ◽  
Live Cells ◽  
Oxygen Species ◽  
Two Photon ◽  
Ratiometric Imaging ◽  
In Cells

A fluorescent probe enables two-photon ratiometric imaging of endogenous hypochlorous acid, a reactive oxygen species, in cells and tissues.

scholarly journals Construction of a lysosome-targetable ratiometric fluorescent probe for H2O2 tracing and imaging in living cells and an inflamed model

RSC Advances ◽  
2021 ◽  
Vol 11 (39) ◽  
pp. 24032-24037
Author(s):  
Rongrong Zhou ◽  
Qiyao Peng ◽  
Dan Wan ◽  
Chao Yu ◽  
Yuan Zhang ◽  
...  
Keyword(s):  
Reactive Oxygen Species ◽  
Hydrogen Peroxide ◽  
Fluorescent Probe ◽  
Reactive Oxygen ◽  
Living Cells ◽  
Oxygen Species ◽  
Oxidation Properties ◽  
Ratiometric Fluorescent Probe ◽  
Destructive Oxidation

Hydrogen peroxide (H2O2), an important reactive oxygen species (ROS) with unique destructive oxidation properties, can be produced in lysosomes to fight off pathogens.

Mitochondria-Targeted Ratiometric Fluorescent Imaging of Cysteine

The Analyst ◽  
2021 ◽  
Author(s):  
Ya-Nan Wei ◽  
Bo Lin ◽  
Yang Shu ◽  
Jian-Hua Wang
Keyword(s):  
Reactive Oxygen Species ◽  
Redox Homeostasis ◽  
Reactive Oxygen ◽  
Fluorescent Imaging ◽  
Oxygen Species ◽  
Cellular Redox ◽  
Critical Part ◽  
Physiological Processes

As an indispensable biothiol, cysteine (Cys) plays a critical part in cellular redox homeostasis, pathological and physiological processes. One of the main sources of reactive oxygen species (ROS) in human...

A charge transfer assisted fluorescent probe for selective detection of hydrogen peroxide among different reactive oxygen species

2012 ◽  
Vol 48 (39) ◽  
pp. 4719 ◽  
Author(s):  
Manoj Kumar ◽  
Naresh Kumar ◽  
Vandana Bhalla ◽  
Parduman Raj Sharma ◽  
Yasrib Qurishi
Keyword(s):  
Reactive Oxygen Species ◽  
Hydrogen Peroxide ◽  
Charge Transfer ◽  
Fluorescent Probe ◽  
Reactive Oxygen ◽  
Selective Detection ◽  
Oxygen Species ◽  
A Charge

Colorimetric and Fluorescent Probe for Hypochlorite with High Selectivity

2013 ◽  
Vol 295-298 ◽  
pp. 475-478 ◽  
Author(s):  
Zhi Xiang Han ◽  
Ming Hui Du ◽  
Guo Xi Liang ◽  
Xiang Yang Wu
Keyword(s):  
Reactive Oxygen Species ◽  
Aqueous Solution ◽  
Detection Limit ◽  
Fluorescent Probe ◽  
Rhodamine B ◽  
High Selectivity ◽  
Reactive Oxygen ◽  
Living Cells ◽  
Oxygen Species ◽  
Turn On

Rhodamine B thiohydrazide (RBS) was firstly employed as turn-on fluorescent probe for hypochlorite in aqueous solution and living cells. It exhibits a stable response to hypochlorite from 1.0×10-6to 1.0×10-5M with a detection limit of 3.3×10-7M. The response of this probe to hypochlorite is fast and highly selective compared with other reactive oxygen species (such as.OH,1O2, H2O2) and other common anions (such as X-, ClO2-, ClO4-, NO3-, NO2-, OH-, Ac-, CO32-, SO42-).

scholarly journals Real-time visualizing the regulation of reactive oxygen species on Zn2+ release in cellular lysosome by a specific fluorescent probe

2018 ◽  
Vol 264 ◽  
pp. 419-425 ◽  
Author(s):  
Zhangjun Hu ◽  
Guanqing Yang ◽  
Jiwen Hu ◽  
Hui Wang ◽  
Peter Eriksson ◽  
...  
Keyword(s):  
Reactive Oxygen Species ◽  
Real Time ◽  
Fluorescent Probe ◽  
Reactive Oxygen ◽  
Oxygen Species

Hypochlorous acid inhibits Ca2+-ATPase from skeletal muscle sarcoplasmic reticulum

1998 ◽  
Vol 84 (2) ◽  
pp. 425-430 ◽  
Author(s):  
Terence G. Favero ◽  
David Colter ◽  
Paul F. Hooper ◽  
Jonathan J. Abramson
Keyword(s):  
Reactive Oxygen Species ◽  
Skeletal Muscle ◽  
Sarcoplasmic Reticulum ◽  
Atpase Activity ◽  
Structural Damage ◽  
Hypochlorous Acid ◽  
Reactive Oxygen ◽  
Sulfhydryl Groups ◽  
Dependent Manner ◽  
Oxygen Species

Favero, Terence G., David Colter, Paul F. Hooper, and Jonathan J. Abramson. Hypochlorous acid inhibits Ca2+-ATPase from skeletal muscle sarcoplasmic reticulum. J. Appl. Physiol. 84(2): 425–430, 1998.—Hypochlorous acid (HOCl) is produced by polymorphonuclear leukocytes that migrate and adhere to endothelial cells as part of the inflammatory response to tissue injury. HOCl is an extremely toxic oxidant that can react with a variety of cellular components, and concentrations reaching 200 μM have been reported in some tissues. In this study, we show that HOCl interacts with the skeletal sarcoplasmic reticulum Ca2+-adenosinetriphosphatase (ATPase), inhibiting transport function. HOCl inhibits sarcoplasmic reticulum Ca2+-ATPase activity in a concentration-dependent manner with a concentration required to inhibit ATPase activity by 50% of 170 μM and with complete inhibition of activity at 3 mM. A concomitant reduction in free sulfhydryl groups after HOCl treatment was observed, paralleling the inhibition of ATPase activity. It was also observed that HOCl inhibited the binding of the fluorescent probe fluorescein isothiocyanate to the ATPase protein, indicating some structural damage may have occurred. These findings suggest that the reactive oxygen species HOCl inhibits ATPase activity via a modification of sulfhydryl groups on the protein, supporting the contention that reactive oxygen species disrupt the normal Ca2+-handling kinetics in muscle cells.

Sign in / Sign up

Export Citation Format

Share Document