Pyrococcus furiosus Argonaute-mediated nucleic acid detection

2019 ◽  
Vol 55 (88) ◽  
pp. 13219-13222 ◽  
Author(s):  
Ruyi He ◽  
Longyu Wang ◽  
Fei Wang ◽  
Wenqiang Li ◽  
Yang Liu ◽  
...  
Keyword(s):  
Nucleic Acid ◽  
Pyrococcus Furiosus ◽  
Nucleic Acid Detection ◽  
Single Nucleotide ◽  
Multiplexed Detection

PfAgo-mediated Nucleic acid Detection (PAND) distinguishes single-nucleotide mutants and accomplishes multiplexed detection by a second round of cleavage.

scholarly journals Nucleic acid detection using a universal electrochemical sensor for SNS differentiation

2018 ◽  
pp. 79-86
Keyword(s):  
Nucleic Acid ◽  
Electrochemical Sensor ◽  
Nucleotide Substitution ◽  
High Sensitivity ◽  
Biomedical Science ◽  
Nucleic Acid Detection ◽  
Single Nucleotide Substitution ◽  
University Of Central Florida ◽  
Single Nucleotide ◽  
Central Florida

Nucleic acid detection using a universal electrochemical sensor for SNS differentiation Detección de ácidos nucleicos usando un sensor electroquímico universal para la discriminación de SNS Percy Calvo-Marzal1, Dmitry M. Kolpashchikov1,2,3 and Karin Y. Chumbimuni-Torres1 1 Department of Chemistry, University of Central Florida, 4000 Central Florida Blvd., Orlando, FL 32816 2 National Center for Forensic Science, University of Central Florida, Orlando, FL 32816 3 Burnett School of Biomedical Science, University of Central Florida, Orlando, FL 32816 Recibido el 26 de diciembre del 2017, aceptado el 31 de diciembre del 2017 DOI: https://doi.org/10.33017/RevECIPeru2017.0008/ Abstract Nucleic acid detection with high sensitivity and selectivity capabilities could aid in the diagnosis of varies diseases such as: infections, cancer, among others. Discrimination of a mismatch or single nucleotide substitution (SNS) is challenge due to a minimum pair to pair formation double strand needed to stabilize the structure. Here we present a new approach to atinge the needed stability by using additional segments strains to favored stabilization. The use of additional complementary strains facilitates the discrimination of a SNS in a cooperative way. Keywords: Nuclei acid, Single nucleotide substitution, infection, cancer

scholarly journals Multiplexed detection of bacterial nucleic acids using Cas13 in droplet microarrays

2021 ◽  
Author(s):  
Sri Gowtham Thakku ◽  
Cheri M Ackerman ◽  
Cameron Myhrvold ◽  
Roby P Bhattacharyya ◽  
Jonathan Livny ◽  
...  
Keyword(s):  
Nucleic Acid ◽  
Sensitivity And Specificity ◽  
Health Management ◽  
Point Of Care ◽  
Bacterial Species ◽  
Bacterial Pathogen ◽  
Antibiotic Resistance Genes ◽  
Detection Methods ◽  
Nucleic Acid Detection ◽  
Multiplexed Detection

Rapid and accurate diagnosis of infections is fundamental to individual patient care and public health management. Nucleic acid detection methods are critical to this effort, but are limited either in the breadth of pathogens targeted or by the expertise and infrastructure required. We present here a high-throughput system that enables rapid identification of bacterial pathogens, bCARMEN, which utilizes: (1) modular CRISPR-Cas13-based nucleic acid detection with enhanced sensitivity and specificity; and (2) a droplet microfluidic system that enables thousands of simultaneous, spatially multiplexed detection reactions at nanoliter volumes; and (3) a novel pre-amplification strategy that further enhances sensitivity and specificity. We demonstrate bCARMEN is capable of detecting and discriminating 52 clinically relevant bacterial species and several key antibiotic resistance genes. We further develop a proof of principle system for use with stabilized reagents and a simple workflow with optical readout using a cell phone camera, opening up the possibility of a rapid point-of-care multiplexed bacterial pathogen identification and antibiotic susceptibility testing.

scholarly journals Single-step multivalent capture assay for nucleic acid detection with dual-affinity regulation using mutation inhibition and allosteric activation

2019 ◽  
Vol 10 (19) ◽  
pp. 5025-5030 ◽  
Author(s):  
Xing Lu ◽  
Guobao Zhou ◽  
Yanbo Zeng ◽  
Zhengzhi Yin ◽  
Zulei Zhang ◽  
...  
Keyword(s):  
Nucleic Acid ◽  
Dynamic Range ◽  
Single Step ◽  
Nucleic Acid Detection ◽  
Allosteric Activation ◽  
Single Nucleotide ◽  
Capture Assay ◽  
Nucleotide Resolution ◽  
Single Nucleotide Resolution

A single-step electrocatalytic biosensor with dual-affinity regulation enables a tunable dynamic range and tunable single nucleotide resolution for nucleic acid detection.

Nucleic acid detection on DNA chips using highly reactive and stable diazo-biotins

2008 ◽  
Author(s):  
Alain Laurent ◽  
Arnaud Burr ◽  
Thibault Martin ◽  
Frédéric Lasnet ◽  
Sébastien Hauser ◽  
...  
Keyword(s):  
Nucleic Acid ◽  
Nucleic Acid Detection ◽  
Dna Chips

Identifying the etiologic role of Parvovirus B19 in non-immune hydrops fetalis by histopathology, immunohistochemistry and nucleic acid testing: a retrospective study

Open Medicine ◽  
2007 ◽  
Vol 2 (3) ◽  
pp. 271-279 ◽  
Author(s):  
Koray Ergunay ◽  
Gulcin Altinok ◽  
Bora Gurel ◽  
Ahmet Pinar ◽  
Arzu Sungur ◽  
...  
Keyword(s):  
Nucleic Acid ◽  
Real Time ◽  
San Francisco ◽  
Real Time Pcr ◽  
Nested Pcr ◽  
Parvovirus B19 ◽  
Etiologic Agent ◽  
Hydrops Fetalis ◽  
Nucleic Acid Detection

AbstractIntrauterine Parvovirus B19 infections may cause fetal anemia, non-immune hydrops fetalis or abortion. This study focuses on the pathogenic role of Parvovirus B19 in non-immune hydrops fetalis at Hacettepe University, a major reference hospital in Turkey. Twenty-two cases of non-immune hydrops fetalis were retrospectively selected out of a total of 431 hydrops fetalis specimens from the Department of Pathology archieves. Paraffine embedded tissue sections from placental and liver tissues from each case were evaluated by histopathology, immunohistochemistry, nested PCR and commercial quantitative Real-time PCR. Viral DNA was detected in placental tissues by Real-time PCR in 2 cases (2/22, 9.1%) where histopathology also revealed changes suggestive of Parvovirus B19 infection. No significant histopathologic changes were observed for the remaining sections. Nested PCR that targets the VP1 region of the viral genome and immunohistochemistry for viral capsid antigens were negative for all cases. As a result, Parvovirus B19 is identified as the etiologic agent for the development of non-immune hydrops fetalis for 9.1% of the cases in Hacettepe University, Turkey. Real-time PCR is observed to be an effective diagnostic tool for nucleic acid detection from paraffine embedded tissues. Part of this study was presented as a poster at XIIIth International Congress of Virology, San Francisco, USA (Abstract V-572).

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