Grape skin fermentation by Lactobacillus fermentum CQPC04 has anti-oxidative effects on human embryonic kidney cells and apoptosis-promoting effects on human hepatoma cells

Background: Recent studies have attempted to elucidate the function of super enhancers by means of microRNAs. Although the functional outcomes of miR-1301 have become clearer, the pathways that regulate the expressions of miR-1301 remain unclear. Objective: The objective of this paper was to consider the pathway regulating expression of miR- 1301 and miR-1301 signaling pathways with the inhibition of cell proliferation. Methods: In this study, we prepared the cell clones that the KLF6 super enhancer was deleted by means of the CRISPR/Cas9 system-mediated genetic engineering. Changes in miR-1301 expression after the deletion of the KLF6 super enhancer were evaluated by RT-PCR analysis, and the signal pathway of miR-1301 with inhibition of the cell proliferation was examined using RNA interference technology. Results: The results showed that miR-1301 expression was significantly increased after the deletion of the KLF6 super enhancer. Over-expression of miR-1301 induced by deletion of the KLF6 super enhancer also regulated the expression of p21 and p53 in human hepatoma cells. functional modeling of findings using siRNA specific to miR-1301 showed that expression level changes had direct biological effects on cellular proliferation in Human hepatoma cells. Furthermore, cellular proliferation assay was shown to be directly associated with miR-1301 levels. Conclusion: As a result, it was demonstrated that the over-expression of miR-1301 induced by the disruption of the KLF6 super enhancer leads to a significant inhibition of proliferation in HepG2 cells. Moreover, it was demonstrated that the KLF6 super enhancer regulates the cell-proliferative effects which are mediated, at least in part, by the induction of p21and p53 in a p53-dependent manner. Our results provide the functional significance of miR-1301 in understanding the transcriptional regulation mechanism of the KLF6 super enhancer.

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Induction of plasminogen activator inhibitor-1 in HepG2 human hepatoma cells by mediators of the acute phase response.

Journal of Biological Chemistry ◽

10.1016/s0021-9258(17)32279-2 ◽

1994 ◽

Vol 269 (29) ◽

pp. 19095-19100 ◽

Cited By ~ 1

Author(s):

A.M. Healy ◽

T.D. Gelehrter

Keyword(s):

Plasminogen Activator ◽

Acute Phase ◽

Acute Phase Response ◽

Plasminogen Activator Inhibitor ◽

Hepatoma Cells ◽

Phase Response ◽

Human Hepatoma ◽

Plasminogen Activator Inhibitor 1 ◽

Human Hepatoma Cells ◽

Activator Inhibitor

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(−)-Epigallocatechin-3-gallate Induces Apoptosis of Human Hepatoma Cells by Mitochondrial Pathways Related to Reactive Oxygen Species

Journal of Agricultural and Food Chemistry ◽

10.1021/jf901396f ◽

2009 ◽

Vol 57 (15) ◽

pp. 6685-6691 ◽

Cited By ~ 41

Author(s):

WenJuan Li ◽

ShaoPing Nie ◽

Qiang Yu ◽

MingYong Xie

Keyword(s):

Reactive Oxygen Species ◽

Reactive Oxygen ◽

Hepatoma Cells ◽

Oxygen Species ◽

Human Hepatoma ◽

Human Hepatoma Cells

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Functional Comparison between VP64-dCas9-VP64 and dCas9-VP192 CRISPR Activators in Human Embryonic Kidney Cells

International Journal of Molecular Sciences ◽

10.3390/ijms22010397 ◽

2021 ◽

Vol 22 (1) ◽

pp. 397

Author(s):

Nasir Javaid ◽

Thuong L. H. Pham ◽

Sangdun Choi

Keyword(s):

Protein Level ◽

Reference Genes ◽

Mrna Level ◽

High Specificity ◽

Kidney Cells ◽

Human Embryonic Kidney ◽

Human Embryonic Kidney Cells ◽

Sox2 Expression

Reversal in the transcriptional status of desired genes has been exploited for multiple research, therapeutic, and biotechnological purposes. CRISPR/dCas9-based activators can activate transcriptionally silenced genes after being guided by gene-specific gRNA(s). Here, we performed a functional comparison between two such activators, VP64-dCas9-VP64 and dCas9-VP192, in human embryonic kidney cells by the concomitant targeting of POU5F1 and SOX2. We found 22- and 6-fold upregulations in the mRNA level of POU5F1 by dCas9-VP192 and VP64-dCas9-VP64, respectively. Likewise, SOX2 was up-regulated 4- and 2-fold using dCas9-VP192 and VP64dCas9VP64, respectively. For the POU5F1 protein level, we observed 3.7- and 2.2-fold increases with dCas9-VP192 and VP64-dCas9-VP64, respectively. Similarly, the SOX2 expression was 2.4- and 2-fold higher with dCas9-VP192 and VP64-dCas9-VP64, respectively. We also confirmed that activation only happened upon co-transfecting an activator plasmid with multiplex gRNA plasmid with a high specificity to the reference genes. Our data revealed that dCas9-VP192 is more efficient than VP64-dCas9-VP64 for activating reference genes.

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