Influence of pore architectures of silk fibroin/collagen composite scaffolds on the regeneration of osteochondral defects in vivo

2020 ◽  
Vol 8 (3) ◽  
pp. 391-405 ◽  
Author(s):  
Xue Feng ◽  
Peifang Xu ◽  
Tao Shen ◽  
Yihan Zhang ◽  
Juan Ye ◽  
...  
Keyword(s):  
Silk Fibroin ◽  
Osteochondral Defects ◽  
Composite Scaffolds

The aligned scaffolds facilitate migration of endogenous reparative cells, leading to better regeneration of osteochondral defects.

scholarly journals Characterization of Macroporous Polycaprolactone/Silk Fibroin/Gelatin/Ascorbic Acid Composite Scaffolds and In Vivo Results in a Rabbit Model for Meniscus Cartilage Repair

Cartilage ◽  
2021 ◽  
pp. 194760352110354
Author(s):  
Zahra Abpeikar ◽  
Lida Moradi ◽  
Moosa Javdani ◽  
Saeid Kargozar ◽  
Mostafa Soleimannejad ◽  
...  
Keyword(s):  
Ascorbic Acid ◽  
Antioxidant Capacity ◽  
Silk Fibroin ◽  
Rabbit Model ◽  
Mechanical Tests ◽  
Toxicity Tests ◽  
Self Healing ◽  
Composite Scaffolds ◽  
Meniscus Healing

Objective Meniscus injuries in the inner avascular zone have weak intrinsic self-healing capacity and often progress to osteoarthritis. This study focused on evaluating the effects of polycaprolactone/silk fibroin/gelatin/ascorbic acid (PCL/SF/Gel/AA) composite scaffolds seeded with adipose-derived mesenchymal stem cells (ASCs), in the meniscus repair. Design To this end, composite scaffolds were cross-linked using N-hydroxysuccinimide and 1-ethyl-3-(3-dimethyl-aminopropyl)-1-carbodiimide hydrochloride. Scaffolds were then characterized by scanning electron microscope, mechanical tests, total antioxidant capacity, swelling, and toxicity tests. Results The PCL/SF/Gel/AA scaffolds exhibited suitable mechanical properties. Furthermore, vitamin C rendered them the highest antioxidant capacity. The PCL/SF/Gel/AA scaffolds also showed good biocompatibility and proliferation for chondrocytes. Moreover, the PCL/SF/Gel/AA scaffold seeded with allogeneic ASCs was engrafted in New Zealand rabbits who underwent unilateral punch defect in the medial meniscus of the right knee. After 2 months postimplantation, macroscopic and histologic studies for new meniscus cartilage were performed. Conclusions Our results indicated that the PCL/SF/Gel/AA composite scaffolds seeded with allogeneic ASCs could successfully improve meniscus healing in damaged rabbits.

Aptamer-Functionalized Bioscaffold Enhances Cartilage Repair by Improving Stem Cell Recruitment in Osteochondral Defects of Rabbit Knees

2019 ◽  
Vol 47 (10) ◽  
pp. 2316-2326 ◽  
Author(s):  
Xin Wang ◽  
Xiongbo Song ◽  
Tao Li ◽  
Jiajia Chen ◽  
Guotao Cheng ◽  
...  
Keyword(s):  
Stem Cells ◽  
Silk Fibroin ◽  
Cartilage Repair ◽  
Osteochondral Defect ◽  
Cartilage Tissue ◽  
Control Group ◽  
Osteochondral Defects ◽  
Scaffold Group

Background: Recruitment of endogenous stem cells has been considered an alternative to cell injection/implantation in articular cartilage repair. Purpose: (1) To develop a cartilage tissue-engineering scaffold with clinically available biomaterials and functionalize the scaffold with an aptamer (Apt19s) that specifically recognizes pluripotent stem cells. (2) To determine whether this scaffold could recruit joint-resident mesenchymal stem cells (MSCs) when implanted into an osteochondral defect in a rabbit model and to examine the effects of cartilage regeneration. Study Design: Controlled laboratory study. Methods: The reinforced scaffold was fabricated by embedding a silk fibroin sponge into silk fibroin/hyaluronic acid–tyramine hydrogel and characterized in vitro. A cylindrical osteochondral defect (3.2 mm wide × 4 mm deep) was created in the trochlear grooves of rabbit knees. The rabbits were randomly assigned into 3 groups: Apt19s-functionalized scaffold group, scaffold-only group, and control group. Animals were sacrificed at 6 and 12 weeks after transplantation. Repaired tissues were evaluated via gross examination, histologic examination, and immunohistochemistry. Results: In vitro, this aptamer-functionalized scaffold could recruit bone marrow–derived MSCs and support cell adhesion. In vivo, the aptamer-functionalized scaffold enhanced cell homing in comparison with the aptamer-free scaffold. The aptamer-functionalized scaffold group also exhibited superior cartilage restoration when compared with the scaffold-only group and the control group. Conclusion: The Apt19s-functionalized scaffold exhibited the ability to recruit MSCs both in vitro and in vivo and achieved a better outcome of cartilage repair than the scaffold only or control in an osteochondral defect model. Clinical Relevance: The findings demonstrate a promising strategy of using aptamer-functionalized bioscaffolds for restoration of chondral/osteochondral defects via aptamer-introduced homing of MSCs.

Evaluation of Structure-Controlled Silk Fibroin Coatings for Orthopedic Infection and In-Situ Osteogenesis: In Vitro and In Vivo

2020 ◽  
Author(s):  
Wenhao Zhou ◽  
Teng Zhang ◽  
Jianglong Yan ◽  
QiYao Li ◽  
Panpan Xiong ◽  
...  
Keyword(s):  
Silk Fibroin ◽  
Orthopedic Infection

scholarly journals A composite scaffold of Wharton’s jelly and chondroitin sulphate loaded with human umbilical cord mesenchymal stem cells repairs articular cartilage defects in rat knee

2021 ◽  
Vol 32 (4) ◽  
Author(s):  
Zhong Li ◽  
Yikang Bi ◽  
Qi Wu ◽  
Chao Chen ◽  
Lu Zhou ◽  
...  
Keyword(s):  
Stem Cells ◽  
Articular Cartilage ◽  
Composite Scaffold ◽  
Biomechanical Properties ◽  
Cartilage Defects ◽  
Human Umbilical Cord ◽  
Composite Scaffolds ◽  
Articular Cartilage Defects

AbstractTo evaluate the performance of a composite scaffold of Wharton’s jelly (WJ) and chondroitin sulfate (CS) and the effect of the composite scaffold loaded with human umbilical cord mesenchymal stem cells (hUCMSCs) in repairing articular cartilage defects, two experiments were carried out. The in vitro experiments involved identification of the hUCMSCs, construction of the biomimetic composite scaffolds by the physical and chemical crosslinking of WJ and CS, and testing of the biomechanical properties of both the composite scaffold and the WJ scaffold. In the in vivo experiments, composite scaffolds loaded with hUCMSCs and WJ scaffolds loaded with hUCMSCs were applied to repair articular cartilage defects in the rat knee. Moreover, their repair effects were evaluated by the unaided eye, histological observations, and the immunogenicity of scaffolds and hUCMSCs. We found that in vitro, the Young’s modulus of the composite scaffold (WJ-CS) was higher than that of the WJ scaffold. In vivo, the composite scaffold loaded with hUCMSCs repaired rat cartilage defects better than did the WJ scaffold loaded with hUCMSCs. Both the scaffold and hUCMSCs showed low immunogenicity. These results demonstrate that the in vitro construction of a human-derived WJ-CS composite scaffold enhances the biomechanical properties of WJ and that the repair of knee cartilage defects in rats is better with the composite scaffold than with the single WJ scaffold if the scaffold is loaded with hUCMSCs.

scholarly journals The use of PLDLA/PCL-T scaffold to repair osteochondral defects in vivo

2012 ◽  
Vol 16 (1) ◽  
pp. 105-115 ◽  
Author(s):  
Andrea Rodrigues Esposito ◽  
Angelo Carneiro Bonadio ◽  
Nathaly Oliveira Pereira ◽  
Túlio Pereira Cardoso ◽  
Maria Lourdes Peris Barbo ◽  
...  
Keyword(s):  
Osteochondral Defects

scholarly journals In vivo degradation of three-dimensional silk fibroin scaffolds

Biomaterials ◽  
2008 ◽  
Vol 29 (24-25) ◽  
pp. 3415-3428 ◽  
Author(s):  
Yongzhong Wang ◽  
Darya D. Rudym ◽  
Ashley Walsh ◽  
Lauren Abrahamsen ◽  
Hyeon-Joo Kim ◽  
...  
Keyword(s):  
Silk Fibroin ◽  
Three Dimensional ◽  
In Vivo Degradation

Characteristics and Osteoconductivity of Bone Composite Scaffolds Made of Thai Silk Fibroin, Gelatin and Inorganic Compounds: A Comparative Study of β-Tricalcium Phosphate and Hydroxyapatite

2015 ◽  
Vol 354 (1) ◽  
pp. 258-264 ◽  
Author(s):  
Chotika Dararutana ◽  
Juthamas Ratanavaraporn ◽  
Sittisak Honsawek ◽  
Sorada Kanokpanont ◽  
Siriporn Damrongsakkul
Keyword(s):  
Comparative Study ◽  
Silk Fibroin ◽  
Tricalcium Phosphate ◽  
Inorganic Compounds ◽  
Composite Scaffolds

scholarly journals Peri-implant cartilage and bone histological changes following treatment of focal osteochondral defects in the femoral head with polyether ether ketone implants versus cobalt chromium molybdenum alloy implants: assessment in a goat model

2020 ◽  
Author(s):  
Zhiguo Yuan ◽  
Wei Zhang ◽  
Xiangchao Meng ◽  
Jue Zhang ◽  
Teng TengLong ◽  
...  
Keyword(s):  
Femoral Head ◽  
Osteochondral Defect ◽  
Osteochondral Defects ◽  
Cobalt Chromium ◽  
Cocrmo Alloy ◽  
Polyether Ether Ketone ◽  
Histological Score ◽  
Ether Ketone ◽  
Cobalt Chromium Molybdenum

Abstract Objective: This study aimed to quantitatively investigate the peri-implant histology of applying defect-size polyether ether ketone (PEEK) implant for the treatment of localized osteochondral defects in the femoral head and compared it with cobalt chromium molybdenum (CoCrMo) alloy implant.Methods: A femoral head osteochondral defect model was created in the left hips of goats (n=12). Defects were randomly treated by immediate placement of a PEEK (n=6) or CoCrMo implant (n=6). The un-operated right hip joints served as a control. Goats were sacrificed at 12 weeks. Periprosthetic cartilage quality was semi-quantitatively analyzed macroscopically and microscopically. Implant osseointegration was measured by micro-CT and histomorphometry.Results: The modified macroscopic articular evaluation score in the PEEK group was lower than that in the CoCrMo group (p<0.05), and the histological score of the periprosthetic and acetabular cartilage in the PEEK group was lower than that in the CoCrMo group (P<0.05). The mean bone-implant contact for PEEK implants was comparable with that for CoCrMo alloy implants at 12 weeks.Conclusions: A PEEK implant for the treatment of local osteochondral defect in the femoral head demonstrated effective fixation and superior in vivo cartilage protection compared with an identical CoCrMo alloy implant.

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