A NIR fluorescent probe based on phenazine with a large Stokes shift for the detection and imaging of endogenous H2O2 in RAW 264.7 cells

The Analyst ◽  
2020 ◽  
Vol 145 (12) ◽  
pp. 4196-4203
Author(s):  
Yongchao Yan ◽  
Lingyan Liu ◽  
Chenglin Li ◽  
Zhicheng Yang ◽  
Tao Yi ◽  
...  
Keyword(s):  
Fluorescent Probe ◽  
Fluorescence Enhancement ◽  
Color Change ◽  
Stokes Shift ◽  
Raw 264.7 Cells ◽  
Raw 264.7 ◽  
Large Stokes Shift

A NIR fluorescent probe PCN-BP with a large Stokes shift was developed. It displayed fluorescence enhancement and color change after H2O2 was added. Moreover, it was employed for the exogenous and endogenous imaging of H2O2 in RAW 264.7 cells.

scholarly journals A Phenothiazine-HPQ Based Fluorescent Probe with a Large Stokes Shift for Sensing Biothiols in Living Systems

Molecules ◽  
2021 ◽  
Vol 26 (8) ◽  
pp. 2337
Author(s):  
Yan Zheng ◽  
Peng Hou ◽  
Yu Li ◽  
Jingwen Sun ◽  
Hongxia Cui ◽  
...  
Keyword(s):  
Fluorescent Probe ◽  
Fluorescence Enhancement ◽  
Stokes Shift ◽  
Redox Properties ◽  
Living Systems ◽  
Selective Detection ◽  
Large Stokes Shift ◽  
Low Cytotoxicity ◽  
Mcf 7

Due to the redox properties closely related to numerous physiological and pathological processes, biothiols, including cysteine (Cys), homocysteine (Hcy) and glutathione (GSH), have received considerable attention in biological science. On account of the important physiological roles of these biothiols, it is of profound significance to develop sensitive and selective detection of biothiols to understand their biological profiles. In this work, we reported an efficient fluorescent probe, PHPQ-SH, for detecting biothiols in vitro and vivo, based on the phenothiazine-HPQ skeleton, with DNBS (2,4-dinitrobenzenesulfonate) as the response unit. Probe PHPQ-SH exhibited brilliant sensing performances toward thiols, including a large Stokes shift (138 nm), excellent sensitivity (for GSH, LOD = 18.3 nM), remarkable fluorescence enhancement (163-fold), low cytotoxicity, rapid response (8 min), and extraordinary selectivity. Finally, the probe PHPQ-SH illustrated herein was capable of responding and visualizing biothiols in MCF-7 cells and zebrafish.

A novel near-infrared fluorescent probe based on isophorone for the bioassay of endogenous cysteine

2021 ◽  
Author(s):  
Hong-Bo Liu ◽  
Hai Xu ◽  
Xin Guo ◽  
Jian Xiao ◽  
Zheng-Hong Cai ◽  
...  
Keyword(s):  
Aqueous Solution ◽  
Fluorescent Probe ◽  
Near Infrared ◽  
Stokes Shift ◽  
Rapid Identification ◽  
Large Stokes Shift

A near-infrared (NIR) fluorescent probe with a large Stokes shift (143 nm) for the rapid identification of Cys over Hcy and GSH in aqueous solution was developed.

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