scholarly journals A Phenothiazine-HPQ Based Fluorescent Probe with a Large Stokes Shift for Sensing Biothiols in Living Systems

Molecules ◽  
2021 ◽  
Vol 26 (8) ◽  
pp. 2337
Author(s):  
Yan Zheng ◽  
Peng Hou ◽  
Yu Li ◽  
Jingwen Sun ◽  
Hongxia Cui ◽  
...  
Keyword(s):  
Fluorescent Probe ◽  
Fluorescence Enhancement ◽  
Stokes Shift ◽  
Redox Properties ◽  
Living Systems ◽  
Selective Detection ◽  
Large Stokes Shift ◽  
Low Cytotoxicity ◽  
Mcf 7

Due to the redox properties closely related to numerous physiological and pathological processes, biothiols, including cysteine (Cys), homocysteine (Hcy) and glutathione (GSH), have received considerable attention in biological science. On account of the important physiological roles of these biothiols, it is of profound significance to develop sensitive and selective detection of biothiols to understand their biological profiles. In this work, we reported an efficient fluorescent probe, PHPQ-SH, for detecting biothiols in vitro and vivo, based on the phenothiazine-HPQ skeleton, with DNBS (2,4-dinitrobenzenesulfonate) as the response unit. Probe PHPQ-SH exhibited brilliant sensing performances toward thiols, including a large Stokes shift (138 nm), excellent sensitivity (for GSH, LOD = 18.3 nM), remarkable fluorescence enhancement (163-fold), low cytotoxicity, rapid response (8 min), and extraordinary selectivity. Finally, the probe PHPQ-SH illustrated herein was capable of responding and visualizing biothiols in MCF-7 cells and zebrafish.

scholarly journals Recognition of Thiols in Living Cells and Zebrafish Using an Imidazo[1,5-α]pyridine-Derivative Indicator

Molecules ◽  
2019 ◽  
Vol 24 (18) ◽  
pp. 3328 ◽  
Author(s):  
Song Chen ◽  
Peng Hou ◽  
Jingwen Sun ◽  
Haijun Wang ◽  
Lei Liu
Keyword(s):  
Response Time ◽  
Fluorescent Probe ◽  
Stokes Shift ◽  
Detection Limits ◽  
Fast Response ◽  
Living Cells ◽  
Pyridine Derivative ◽  
Selective Detection ◽  
Low Cytotoxicity ◽  
Mcf 7

A new cyan fluorescent probe, MIPY-DNBS, using an imidazo[1,5-α]pyridine derivative as the fluorophore and 2,4-dinitrobenzensufonate as the recognition site for the selective detection of thiols (Cys, GSH, and Hcy), was designed and synthesized. Probe MIPY-DNBS exhibited a 172 nm Stokes shift, a fast response time (400 s), low cytotoxicity, low detection limits (12.7 nM for Cys), and excellent selectively in the detection of thiols. In addition, MIPY-DNBS was successfully applied to imaging thiols in living MCF-7 cells and zebrafish.

A NIR fluorescent probe based on phenazine with a large Stokes shift for the detection and imaging of endogenous H2O2 in RAW 264.7 cells

The Analyst ◽  
2020 ◽  
Vol 145 (12) ◽  
pp. 4196-4203
Author(s):  
Yongchao Yan ◽  
Lingyan Liu ◽  
Chenglin Li ◽  
Zhicheng Yang ◽  
Tao Yi ◽  
...  
Keyword(s):  
Fluorescent Probe ◽  
Fluorescence Enhancement ◽  
Color Change ◽  
Stokes Shift ◽  
Raw 264.7 Cells ◽  
Raw 264.7 ◽  
Large Stokes Shift

A NIR fluorescent probe PCN-BP with a large Stokes shift was developed. It displayed fluorescence enhancement and color change after H2O2 was added. Moreover, it was employed for the exogenous and endogenous imaging of H2O2 in RAW 264.7 cells.

A novel near-infrared and naked-eyes turn on fluorescent probe for detection of biothiols with a large Stokes shift and its application in living cells

2018 ◽  
Vol 10 (32) ◽  
pp. 3991-3999 ◽  
Author(s):  
Wang Zhang ◽  
Gu Weijing ◽  
Tian Cheng ◽  
Bingxiang Wang ◽  
Yuliang Jiang ◽  
...  
Keyword(s):  
Amino Acids ◽  
Fluorescent Probe ◽  
Near Infrared ◽  
Stokes Shift ◽  
Living Cells ◽  
Selective Detection ◽  
Large Stokes Shift ◽  
Turn On

A novel turn-on fluorescent probe (DDND) for highly selective detection of biothiols over other amino acids was synthesized and investigated in this work, which used the 2,4-dinitrobenzenesulfonyl group as a fluorescent quencher.

A red-emitting fluorescent probe for the detection of Hg2+ in aqueous medium, living cells and organisms with a large Stokes shift

2018 ◽  
Vol 16 (27) ◽  
pp. 5036-5042 ◽  
Author(s):  
Lei Yang ◽  
Yuanan Su ◽  
Yani Geng ◽  
Haiqing Xiong ◽  
Jinliang Han ◽  
...  
Keyword(s):  
Detection Limit ◽  
Aqueous Solutions ◽  
Aqueous Medium ◽  
Fluorescent Probe ◽  
Stokes Shift ◽  
Living Cells ◽  
Selective Detection ◽  
Red Emission ◽  
Large Stokes Shift ◽  
Low Detection Limit

A vinethene-based fluorescent probe has been developed for the sensitive and selective detection of Hg2+ with a low detection limit, a red emission and a large Stokes shift. The probe has been successfully applied to detect Hg2+ in aqueous solutions, living cells and organisms.

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