Fluorogenic probes for mitochondria and lysosomes via intramolecular photoclick reaction

The Analyst ◽  
2021 ◽  
Author(s):  
Song Liu ◽  
Han Su ◽  
Lingli Bu ◽  
Jiangyu Yan ◽  
Guorui Li ◽  
...  
Keyword(s):  
Fluorescence Imaging ◽  
Click Reaction ◽  
Living Cells ◽  
Fluorogenic Probes

Probes based on the intramolecular tetrazole-ene photo-click reaction were developed for the in situ fluorescence imaging of mitochondria and lysosomes in living cells.

scholarly journals Protein-AMPylierungs-Identifikation in lebenden Zellen

BIOspektrum ◽  
2020 ◽  
Vol 26 (7) ◽  
pp. 743-746
Author(s):  
Tobias Becker ◽  
Pavel Kielkowski
Keyword(s):  
Endoplasmic Reticulum ◽  
Endoplasmic Reticulum Stress ◽  
Neural Development ◽  
Click Reaction ◽  
Living Cells ◽  
Fluorescence Labeling ◽  
Post Translational Modification ◽  
Design Synthesis ◽  
Stress Regulation

AbstractProtein AMPylation is a prevalent protein post-translational modification in human cells involved in endoplasmic reticulum stress regulation and neural development. In this article we describe the design, synthesis and application of a pronucleotide probe suitable for in situ fluorescence imaging and chemical protemics profiling of AMPylated proteins. Our probe utilizes straightforward strain-promoted azidealkyne click reaction for fluorescence labeling in living cells.

8 Sydnone-Based Cycloadditions in Click Chemistry

2022 ◽  
Author(s):  
F. Friscourt
Keyword(s):  
Drug Discovery ◽  
Click Chemistry ◽  
Click Reaction ◽  
Living Cells ◽  
Dipolar Cycloaddition ◽  
Metal Catalysis ◽  
Metal Free ◽  
Fluorogenic Probes ◽  
Release Strategy

AbstractThe 1,3-dipolar cycloaddition of sydnones (1,2,3-oxadiazolium-5-olates) with dipolarophiles, such as alkynes, has recently emerged as a versatile click reaction, with applications ranging from the mild and regioselective preparation of polysubstituted pyrazoles for drug discovery to the metal-free bioorthogonal ligation of biomacromolecules in living cells. This chapter reviews the importance of metal catalysis for controlling the regioselectivity of the copper-mediated reaction (CuSAC), as well as the development of fluorogenic probes, the click and release strategy, and photo-triggered ligations based on strain-promoted sydnone–alkyne cycloadditions (SPSAC).

DNA nanolantern-based split aptamer probes for in-situ ATP imaging in living cells and lighting up mitochondria

The Analyst ◽  
2021 ◽  
Author(s):  
Yaxin Wang ◽  
Dong-Xia Wang ◽  
Jia-Yi Ma ◽  
Jing Wang ◽  
Yichen Du ◽  
...  
Keyword(s):  
Adenosine Triphosphate ◽  
Cell Metabolism ◽  
Living Cells ◽  
Expression Level ◽  
Specific Analysis

Accurate and specific analysis of adenosine triphosphate (ATP) expression level in living cells can provide valuable information for understanding cell metabolism, physiological activities and pathologic mechanism. Herein, DNA nanolantern-based split...

DNA‐Compatible Click Reaction Employing In Situ Generated Azides from Boronic Acids

2021 ◽  
Vol 1 (5) ◽  
Author(s):  
Yulong An ◽  
Hao Yan ◽  
Zhenzhen Dong ◽  
Alexander L. Satz
Keyword(s):  
Click Reaction ◽  
Boronic Acids
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