The length difference and low flexibility of cotton stalk bark fibers make fiber spinning difficult. In this work, cotton stalk bark fibers that were directly from degummed cotton stalk bark were wetted, carded and softened before sliver-marking, and then they were blended with cotton fibers for carding and spinning in various blending ratios. The results showed that a reasonable open roller speed was 6000 rpm for blending ratio 50/50, 7000 rpm for blending ratio 35/65, and 8000 rpm for blending ratio 20/80. The minimum yarn linear density was 60 tex for blending ratio 50/50, 50 tex for blending ratio 35/65, and 40 tex for blending ratio 20/80.