An automated and portable antimicrobial susceptibility testing system for urinary tract infections

Lab on a Chip ◽  
2021 ◽  
Vol 21 (4) ◽  
pp. 755-763
Author(s):  
Kuo-Wei Hsu ◽  
Wen-Bin Lee ◽  
Huey-Ling You ◽  
Mel S. Lee ◽  
Gwo-Bin Lee
Keyword(s):  
Urinary Tract ◽  
Minimum Inhibitory Concentration ◽  
Urinary Tract Infections ◽  
Antimicrobial Susceptibility ◽  
Susceptibility Testing ◽  
Inhibitory Concentration ◽  
Microfluidic System ◽  
Antimicrobial Susceptibility Testing ◽  
Testing System ◽  
Tract Infections

A portable, integrated microfluidic system capable of automatically conducting antimicrobial susceptibility testing (AST) and minimum inhibitory concentration (MIC) measurements using urine samples were developed.

scholarly journals Diagnosis of Urinary Tract Infections in Children

2016 ◽  
Vol 54 (9) ◽  
pp. 2233-2242 ◽  
Author(s):  
Christopher D. Doern ◽  
Susan E. Richardson
Keyword(s):  
Urinary Tract ◽  
Urinary Tract Infections ◽  
Antimicrobial Susceptibility ◽  
Susceptibility Testing ◽  
Laboratory Diagnosis ◽  
Antimicrobial Susceptibility Testing ◽  
Special Consideration ◽  
Common Occurrence ◽  
Specimen Collection ◽  
Tract Infections

Urinary tract infections (UTIs) are a common occurrence in children. The management and laboratory diagnosis of these infections pose unique challenges that are not encountered in adults. Important factors, such as specimen collection, urinalysis interpretation, culture thresholds, and antimicrobial susceptibility testing, require special consideration in children and will be discussed in detail in the following review.

scholarly journals Antibiotic resistance among Escherichia coli urinary isolates and their susceptibility to clove essential oil

2018 ◽  
Vol 71 (2) ◽  
pp. 41
Author(s):  
Michał Dąbrowski ◽  
Monika Sienkiewicz ◽  
Hanna Zielińska-Bliźniewska ◽  
Marta Dąbrowska ◽  
Małgorzata Seredyńska ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Urinary Tract ◽  
Essential Oil ◽  
Urinary Tract Infections ◽  
Antimicrobial Susceptibility ◽  
Susceptibility Testing ◽  
Antimicrobial Susceptibility Testing ◽  
Diffusion Method ◽  
Syzygium Aromaticum ◽  
Tract Infections

<p>Escherichia coli is a Gram-negative, facultatively anaerobic, rod-shaped, coliform bacterium, which is a primary cause of urinary tract infections. Resistance to antibiotics has become a particular problem in recent decades. Consequently, there is an unmet need for new therapeutic options. It has been observed that essential oils have bactericidal effects. The antimicrobial susceptibility testing for Escherichia coli isolates obtained from urine of patients with urinary tract infections was determined via disk diffusion method according to the European Committee on Antimicrobial Susceptibility Testing (EUCAST, 2015). Essential oil from clove – Syzygium aromaticum (L.) Merill et L.M. (Myrtaceae) was analyzed by GC-FID-MS. Minimal Inhibitory Concentration (MIC) and Minimal Bactericidal Concentration (MBC) were detected by using the micro-dilution broth method. Escherichia coli clinical isolates are characterized by high resistance to ampicillin, amoxicillin with clavulanic acid, norfloxacin, trimethoprim/sulfamethoxazole, tetracycline, tobramycin and ticarcillin. Clove oil possesses strong inhibiting and killing properties against E. coli isolates, among them the ones resistant to recommended antibiotics. The results of this study highlight the need for testing the efficacy of new agents to inactivate bacteria in clinical settings.</p>

A multiplexed nanoliter array-based microfluidic platform for quick, automatic antimicrobial susceptibility testing

Lab on a Chip ◽  
2021 ◽  
Author(s):  
Mohammad Osaid ◽  
Yi-Sin Chen ◽  
Chih-Hung Wang ◽  
Anirban Sinha ◽  
Wen-Bin Lee ◽  
...  
Keyword(s):  
Minimum Inhibitory Concentration ◽  
Antimicrobial Susceptibility ◽  
Susceptibility Testing ◽  
Inhibitory Concentration ◽  
Antimicrobial Susceptibility Testing ◽  
Bacterial Cells ◽  
Microfluidic Platform

A nanoliter array-based automatic microfluidic platform for performing rapid antimicrobial susceptibility testing using only ∼2000 bacterial cells was reported, and required 8–9 hours to determine the minimum inhibitory concentration value.

Change in antimicrobial susceptibility of Escherichia coli urinary tract isolates at a single institution over a period of 10 years

2012 ◽  
Vol 58 (3) ◽  
pp. 345-349 ◽  
Author(s):  
A. Walkty ◽  
P.R.S. Lagacé-Wiens ◽  
J.A. Karlowsky ◽  
D.J. Hoban ◽  
K. Manickam ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Urinary Tract ◽  
Urinary Tract Infections ◽  
Antimicrobial Susceptibility ◽  
Susceptibility Testing ◽  
Single Institution ◽  
Entire Study ◽  
E Coli ◽  
Catheter Urine ◽  
Tract Infections

Urinary tract infections are common. Few published studies have demonstrated the change in Escherichia coli urinary isolate antimicrobial susceptibility over time within a given area and (or) population. The purpose of this study was to evaluate the change in susceptibility of E. coli clinical isolates obtained from urine specimens at a single institution over a period of 10 years. The microbiology laboratory information system at St. Boniface Hospital (Winnipeg, Manitoba, Canada) was searched retrospectively from 1 January 2000 to 31 December 2009, for all E. coli isolates from either a midstream or catheter urine source that had susceptibility testing performed. Only one isolate per patient was included during the entire study period. Antimicrobial susceptibility testing was carried out with either a Microscan instrument (pre-April 2004) or a Vitek instrument (May 2004 onwards). In total, 7353 E. coli urinary isolates were included for evaluation. Ciprofloxacin susceptibility declined significantly, from 99% in 2000 to 85% in 2009 (p < 0.0001). A small but statistically significant decline in susceptibility was also observed for ampicillin, cefazolin, trimethoprim–sulfamethoxazole, gentamicin, and nitrofurantoin. These data suggest that certain antimicrobials recommended for the treatment of urinary tract infections (ciprofloxacin, trimethoprim–sulfamethoxazole) may no longer be optimal.

scholarly journals Susceptibility profile of Brazilian Rhodococcus equi isolates against azithromycin, clarithromycin and erythromycin

2015 ◽  
Vol 45 (4) ◽  
pp. 680-683
Author(s):  
Letícia Trevisan Gressler ◽  
Bibiana Petri da Silveira ◽  
Marcelo Luís Schwab ◽  
Agueda Castagna de Vargas ◽  
Luciana Pötter ◽  
...  
Keyword(s):  
Minimum Inhibitory Concentration ◽  
Antimicrobial Susceptibility ◽  
Susceptibility Testing ◽  
Inhibitory Concentration ◽  
Rhodococcus Equi ◽  
Antimicrobial Susceptibility Testing ◽  
Microbiological Culture ◽  
Infection Treatment

Rhodococcus equi infection treatment is usually a macrolide (azithromycin - AZM, clarithromycin - CLR and erythromycin - ERY) and rifampicin combination. However, resistance cases have been reported, especially for ERY. In view of the need of a study about Brazilian isolates susceptibility profile, this study aimed to characterize the minimum inhibitory concentration (MIC) of the macrolides - AZM, CLR and ERY - against 44 R. equi isolates. It was found all isolates CLR and AZM sensitive; however, for ERY, 27% (12/44) were classified as intermediate sensitivity. R. equi Brazilian isolates used here showed a large susceptibility profile, except against ERY, for which it was observed some resistance evidence. In order to avoid failures in the equine rhodococcosis therapy it was highlighted the importance of microbiological culture and antimicrobial susceptibility testing in vitro before beginning treatment

scholarly journals Detection of Antibiotic Susceptibility by Colorimetric Minimum Inhibitory Concentration in Staphylococcal Isolates

QJM ◽  
2020 ◽  
Vol 113 (Supplement_1) ◽  
Author(s):  
B S Mahmoud ◽  
S A ElMasry ◽  
N A Fahim ◽  
O A Shaker ◽  
M A Abdelsattar
Keyword(s):  
Minimum Inhibitory Concentration ◽  
Antimicrobial Susceptibility ◽  
Antibiotic Susceptibility ◽  
Susceptibility Testing ◽  
Inhibitory Concentration ◽  
Antimicrobial Susceptibility Testing ◽  
Bacterial Concentration ◽  
Tetrazolium Chloride ◽  
Wide Range ◽  
Agar Dilution

Abstract Background Staphylococcus species are implicated in a wide range of community and hospital-acquired infections, which necessitates rapid provision of antibiotic susceptibility results for patients at risk. The current study evaluated the usefulness of applying broth microdilution (BMD) and agar dilution methods for antimicrobial susceptibility testing of Staphylococci using tetrazolium salts as redox indicators. Methods Minimum inhibitory concentration (MICs) of eight antimicrobials representing different antibiotic classes as recommended by CLSI; vancomycin (VA), linezolid (LZD), oxacillin (OX), gentamicin (CN), tetracycline (TE), ciprofloxacin (CIP), erythromycin (E) and clindamycin (DA) was investigated for 80 isolates of Staphylococci by a modified BMD with the addition of dimethyl thiazole diphenyl tetrazolium bromide (MTT). As well as agar dilution with the addition of MTT & triphenyl tetrazolium chloride (TTC) at the standard bacterial concentration together with addition of MTT at an experimental bacterial concentration. Results BMD (MTT) showed the highest agreement regarding most antibiotics among different isolates in comparison with the standard BMD. All performed methods showed significant agreement except agar dilution (TTC) using TE, DA, E, and agar dilution (MTT 108) using DA as well as agar dilution (MTT 104) using E among S. aureus. Conclusions Colorimetric BMD was rapid, easy to interpret and showed the highest agreement with the standard BMD. Colorimetric agar dilution (MTT) was less tedious than BMD. The colorimetric MIC method using MTT may be a useful surrogate of antimicrobial susceptibility testing among Staphylococcus isolates.

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