Ultrasensitive chemiluminescence immunoassay with enhanced precision for the detection of cTnI amplified by acridinium ester-loaded microspheres and internally calibrated by magnetic fluorescent nanoparticles

Nanoscale ◽  
2021 ◽  
Vol 13 (5) ◽  
pp. 3275-3284
Author(s):  
Huan Zhao ◽  
Qifeng Lin ◽  
Li Huang ◽  
Yunfeng Zhai ◽  
Yuan Liu ◽  
...  
Keyword(s):  
Human Body ◽  
Accurate Diagnosis ◽  
Fluorescent Nanoparticles ◽  
Internal Standards ◽  
Chemiluminescence Immunoassay ◽  
Potential Signal ◽  
Acridinium Ester ◽  
Hydrogel Microspheres

Hydrogel microspheres sensitive to temperature as new potential signal enhancers and magnetic fluorescent nanoparticles as internal standards were used to establish a new CLIA method for the accurate diagnosis of cTnI in the human body.

Chemiluminescence immunoassay of thyrotropin with acridinium-ester-labeled antibody evaluated and compared with two other immunoassays.

1987 ◽  
Vol 33 (11) ◽  
pp. 2096-2100 ◽  
Author(s):  
M P Bounaud ◽  
J Y Bounaud ◽  
M H Bouin-Pineau ◽  
L Orget ◽  
F Begon
Keyword(s):  
Detection Limit ◽  
Thyroid Function ◽  
Analytical Performance ◽  
Immunoradiometric Assay ◽  
Chemiluminescence Immunoassay ◽  
Hypothyroid Patients ◽  
Hyperthyroid Patients ◽  
Acridinium Ester

Abstract A new chemiluminometric immunoassay of thyrotropin (TSH) involves antibody labeled with acridinium ester ("Magic Lite System," Ciba Corning Diagnostic Corp.). The assay is rapid, with two incubations totaling 2.5 h, requires two standards per run, and takes 10 s per sample for the quantification step. Analytical performance, within- and between-run reproducibilities, and linearity were excellent. The detection limit is 0.04 milli-int. unit/L. Results correlated well with those obtained by immunoradiometric assay (RIA-gnost hTSH, Hoechst-Behring) and immunofluorometric assay (hTSH Delfia, LKB): r = 0.975. TSH measurements in 32 euthyroid subjects ranged from 0.4 to 4.8 milli-int. units/L (mean 1.35 milli-int. units/L). TSH values for 51 hypothyroid and subclinically hypothyroid patients ranged from 2 to 65 milli-int. units/L. TSH values for 33 hyperthyroid patients (less than 0.14 milli-int. unit/L, less than 0.04 milli-int. unit/L in 16 of the 33) were clearly lower than for most untreated euthyroid subjects. For 169 other individuals whose thyroid function was being routinely assessed. TSH ranged from 0.4 to 4.8 milli-int. units/L, three had TSH less than 0.14 milli-int. unit/L, and four had TSH between 0.14 and 0.4 milli-int. unit/L. This system is as efficient and reliable for screening for thyroid function as the two comparison systems.

Chemiluminescence Immunoassay for Progesterone in Plasma Incorporating Acridinium Ester Labelled Antigen

1988 ◽  
Vol 25 (1) ◽  
pp. 27-34 ◽  
Author(s):  
S A Miller ◽  
M S Morton ◽  
A Turkes
Keyword(s):  
Mass Spectrometry ◽  
Cyanogen Bromide ◽  
Solid Phase ◽  
Gas Chromatography Mass Spectrometry ◽  
Chemiluminescence Immunoassay ◽  
Validation Criteria ◽  
Bovine Serum Albumin Conjugate ◽  
Assay Tube ◽  
Acridinium Ester ◽  
Good Agreement

A sensitive, solid-phase chemiluminescence immunoassay suitable for determining progesterone concentrations in plasma has been developed. The solid-phase antiserum was prepared by coupling a monoclonal progesterone-antibody, raised against a progesterone-11α-hemisuccinyl/bovine serum albumin conjugate, to cyanogen bromide activated cellulose. An 11α-progesteryl-2-carboxymethyltyramine-4-(10-methyl)-acridinium-9-carboxylate conjugate was used as the chemiluminescent label. The assay had a lower limit of sensitivity of 3 pg/assay tube and satisfied accepted validation criteria. Progesterone concentrations determined by chemiluminescence assay were in good agreement not only with a radioimmunoassay in routine use but also with a gas chromatography-mass spectrometry procedure.

A Rapid and Sensitive Chemiluminescent Immunoassay of Bisphenol a with NSP-SA-NHS-Labeled

2014 ◽  
Vol 707 ◽  
pp. 7-11
Author(s):  
Fan Fan Yang ◽  
Li Xin Zhu ◽  
Long Xu ◽  
Ren Rong Liu ◽  
Yan Fan ◽  
...  
Keyword(s):  
Bisphenol A ◽  
Inhibitory Concentration ◽  
Limit Of Detection ◽  
Uv Spectrum ◽  
Chemiluminescence Immunoassay ◽  
Highly Sensitive ◽  
Chemiluminescent Immunoassay ◽  
Inhibition Curve ◽  
Sensitive Method ◽  
Acridinium Ester

A novel chemiluminescence immunoassay (CLIA) of Bisphenol A (BPA) with the acridinium ester of NSP-SA-NHS-labeled has been developed. In this study, BVA and NSP-SA-NHS had been coupled with BSA, the UV spectrum results indicated the conjugates which were successfully synthesized. Basing on these luminescence data the inhibition curve of BPA was established, then the linear arrang of the curve was between 0.4 ng/ml and 5 ng/ml, the 50% inhibitory concentration (IC50) was 2.3ng/ml, the lowest limit of detection was 0.1ng/ml, which showed it’s an efficient and highly sensitive method.

Medical Video Processing

2017 ◽  
pp. 1-17
Author(s):  
Srijan Goswami ◽  
Urmimala Dey ◽  
Payel Roy ◽  
Amira Ashour ◽  
Nilanjan Dey
Keyword(s):  
High Resolution ◽  
Data Quality ◽  
Human Body ◽  
Video Processing ◽  
Time Frame ◽  
Medical Data ◽  
Accurate Diagnosis ◽  
Graphical Information ◽  
Clinical Environments ◽  
3D Scanners

In today's medical environments, imaging technology is extremely significant to provide information for accurate diagnosis. An increasing amount of graphical information from high resolution 3D scanners is being used for diagnoses. Improved medical data quality become one of the major aims of researchers. This leads to the development of various medical modalities supported by cameras that can provide videos for the human body internal for surgical purposes and more information for accurate diagnosis. The current chapter studied concept of the video processing, and its application in the medical domain. Based on the highlighted literatures, it is convinced that video processing and real time frame will have outstanding value in the clinical environments.

Chemiluminescence immunoassay of plasma progesterone, with progesterone-acridinium ester used as the labeled antigen.

1985 ◽  
Vol 31 (10) ◽  
pp. 1664-1668 ◽  
Author(s):  
A P Richardson ◽  
J B Kim ◽  
G J Barnard ◽  
W P Collins ◽  
F McCapra
Keyword(s):  
Solid Phase ◽  
Light Yield ◽  
Progesterone Concentration ◽  
Chemiluminescence Immunoassay ◽  
Plasma Progesterone ◽  
Binding Reaction ◽  
Dilute Sodium Hydroxide ◽  
Plasma Extract ◽  
Acridinium Ester ◽  
Venous Plasma

Abstract This simple solid-phase chemiluminescence immunoassay for measurement of progesterone in extracts of venous plasma has sensitivity and precision similar to that of conventional radioimmunoassay with use of a tritiated antigen. The labeled antigen, 11 alpha-progesteryl-2-succinoyltyramine-4-(10-methyl)-acridini um-9-carboxylate, and a monoclonal antibody to progesterone-11 alpha-succinyl-bovine serum albumin are incubated with a 100-microL aliquot of plasma extract (equivalent to 20 microL of plasma) and 50 microL of a suspension of an IgG fraction of a donkey antiserum to mouse immunoglobulins, covalently attached to cellulose particles. After the antibody-binding reaction (60 min at 4 degrees C), 1 mL of phosphate buffer is added to each tube, the tubes are centrifuged (5 min, 1500 X g), and the supernatant fluid is aspirated. The washing step is repeated and diluted hydrochloric acid (50 mmol/L, 50 microL) is added to the pellet. Luminescence is initiated by oxidation with dilute sodium hydroxide/hydrogen peroxide. The signal is integrated over 10 s. The light yield is inversely proportional to the progesterone concentration in the standard or sample.

Medical Video Processing

2018 ◽  
pp. 1709-1725
Author(s):  
Srijan Goswami ◽  
Urmimala Dey ◽  
Payel Roy ◽  
Amira S. Ashour ◽  
Nilanjan Dey
Keyword(s):  
High Resolution ◽  
Data Quality ◽  
Human Body ◽  
Video Processing ◽  
Time Frame ◽  
Medical Data ◽  
Accurate Diagnosis ◽  
Graphical Information ◽  
Clinical Environments ◽  
3D Scanners

In today's medical environments, imaging technology is extremely significant to provide information for accurate diagnosis. An increasing amount of graphical information from high resolution 3D scanners is being used for diagnoses. Improved medical data quality become one of the major aims of researchers. This leads to the development of various medical modalities supported by cameras that can provide videos for the human body internal for surgical purposes and more information for accurate diagnosis. The current chapter studied concept of the video processing, and its application in the medical domain. Based on the highlighted literatures, it is convinced that video processing and real time frame will have outstanding value in the clinical environments.

Simulation of potential action of stimulated external alternating current (AC) in nerve cells using Hindmarsh-Rose model

2021 ◽  
Vol 1 (1) ◽  
pp. 016-022
Author(s):  
Siti Aisah Sekar Arum ◽  
Yuyun Yueniwati ◽  
Mauludi Ariesto Pamungkas
Keyword(s):  
Membrane Potential ◽  
Programming Language ◽  
Human Body ◽  
Cell Response ◽  
Nerve Cells ◽  
Frequency Variation ◽  
Simulation Results ◽  
Matlab Programming ◽  
The Given ◽  
Potential Signal

This paper describes the simulation of potential actions that occur in nerve cells when given frequency variation as a stimulus. The simulation aims to determine the response of nerve cells to various combinations of currents in this case frequency. The simulation results showed that oscillations occur when the given frequency is 0.045 Hz. While the neural cell response occurs when the frequency between 0.055 Hz-0.065 Hz. The method carried out by the author is to modify the model of Hindmarsh-Rose nerve cells and then simulate it using Matlab programming language. Based on the research that has been done it can be concluded that at a frequency of 0.055 Hz a potential signal of action is formed and oscillates. In the human body, nerve cells and muscle cells are cells that have the ability of excitability (stimulated) because it has membrane potential. Short fluctuations of such cells, serve as electrical signals.

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