Chemiluminescence immunoassay of thyrotropin with acridinium-ester-labeled antibody evaluated and compared with two other immunoassays.

1987 ◽  
Vol 33 (11) ◽  
pp. 2096-2100 ◽  
Author(s):  
M P Bounaud ◽  
J Y Bounaud ◽  
M H Bouin-Pineau ◽  
L Orget ◽  
F Begon
Keyword(s):  
Detection Limit ◽  
Thyroid Function ◽  
Analytical Performance ◽  
Immunoradiometric Assay ◽  
Chemiluminescence Immunoassay ◽  
Hypothyroid Patients ◽  
Hyperthyroid Patients ◽  
Acridinium Ester

Abstract A new chemiluminometric immunoassay of thyrotropin (TSH) involves antibody labeled with acridinium ester ("Magic Lite System," Ciba Corning Diagnostic Corp.). The assay is rapid, with two incubations totaling 2.5 h, requires two standards per run, and takes 10 s per sample for the quantification step. Analytical performance, within- and between-run reproducibilities, and linearity were excellent. The detection limit is 0.04 milli-int. unit/L. Results correlated well with those obtained by immunoradiometric assay (RIA-gnost hTSH, Hoechst-Behring) and immunofluorometric assay (hTSH Delfia, LKB): r = 0.975. TSH measurements in 32 euthyroid subjects ranged from 0.4 to 4.8 milli-int. units/L (mean 1.35 milli-int. units/L). TSH values for 51 hypothyroid and subclinically hypothyroid patients ranged from 2 to 65 milli-int. units/L. TSH values for 33 hyperthyroid patients (less than 0.14 milli-int. unit/L, less than 0.04 milli-int. unit/L in 16 of the 33) were clearly lower than for most untreated euthyroid subjects. For 169 other individuals whose thyroid function was being routinely assessed. TSH ranged from 0.4 to 4.8 milli-int. units/L, three had TSH less than 0.14 milli-int. unit/L, and four had TSH between 0.14 and 0.4 milli-int. unit/L. This system is as efficient and reliable for screening for thyroid function as the two comparison systems.

A sensitive and practical immunoradiometric assay of thyrotropin.

1986 ◽  
Vol 32 (3) ◽  
pp. 514-518 ◽  
Author(s):  
T Helenius ◽  
S Tikanoja
Keyword(s):  
Monoclonal Antibodies ◽  
Thyroid Cancer ◽  
Detection Limit ◽  
Cancer Patients ◽  
The Other ◽  
Antigenic Determinants ◽  
Immunoradiometric Assay ◽  
Chloramine T ◽  
Hyperthyroid Patients ◽  
Mean Concentration

Abstract We describe a two-site immunoradiometric assay for thyrotropin (TSH) in serum, based on use of two monoclonal antibodies directed against two separate antigenic determinants on the TSH molecule. One antibody is immobilized on polystyrene beads; the other is radioiodinated by a modified Chloramine T method. The detection limit of the assay is 0.02 milli-int. unit/L. The working range (CV less than 10%) is from 0.1 to greater than 50 milli-int. units/L. The log mean concentration of TSH in sera collected from 100 euthyroid subjects between 08:00 and 11:00 hours was 1.9 milli-int. units/L, the range 0.4-5.4 milli-int. units/L. Values for hyperthyroid patients and thyroid-cancer patients being treated with thyroxin were much lower than those for euthyroid persons. Results by this new assay correlated excellently with those by our conventional radioimmunoassay (r = 0.99) and also with a sensitive immunofluorometric TSH method (Delfia TSH) (r = 0.99).

Laboratory and Diagnostic Performance of a Coated Well Immunoradiometric Assay for Serum Thyrotrophin

1987 ◽  
Vol 24 (3) ◽  
pp. 273-278 ◽  
Author(s):  
S M Gow ◽  
K Nicol ◽  
J Seth ◽  
G Caldwell ◽  
A D Toft ◽  
...  
Keyword(s):  
Thyroid Function ◽  
Thyroid Disorder ◽  
Immunoradiometric Assay ◽  
First Line ◽  
Serum Samples ◽  
Assay Sensitivity ◽  
Line Test ◽  
The Mean ◽  
Hypothyroid Patients ◽  
Overt Hyperthyroidism

A new, coated well immunoradiometric assay (IRMA) for thyrotrophin (TSH) in serum has been evaluated with a view to its use as a first-line test of thyroid function. The Amerwell TSH IRMA is simple, rapid to perform (2·5 h) and the assay sensitivity was 0·07 mU/L with a working range (intra-assay CV <10%) of 0·3–100 mU/L. The mean inter-assay CV was 6·6% for TSH concentrations of 0·30–30·7 mU/L. The method compared favourably with an in-house TSH radioimmunoassay and an alternative commercial IRMA. In consecutive referrals to a thyroid clinic all patients with overt hyperthyroidism ( n = 103) had undetectable TSH concentrations and in those with subclinical hyperthyroidism ( n = 14), TSH was undetectable in 10 and below the reference range in four. The 95% confidence interval for 63 euthyroid serum samples was 0·36–4·3 mU/L. All hypothyroid patients ( n = 20) had increased TSH concentrations. TSH concentrations in pregnancy did not differ significantly from euthyroid TSH values. From 1916 routine tests, 13 undetectable TSH values were found in which thyroid hormone levels were normal and the patients had no known thyroid disorder. The assay appears suitable as a first-line test of thyroid function, but further assessment in a routine laboratory setting is required.

THYROID FUNCTION AND PLASMA CYCLIC AMP RESPONSE TO INTRAVENOUS GLUCAGON

1977 ◽  
Vol 85 (4) ◽  
pp. 760-768 ◽  
Author(s):  
S. Nistrup Madsen
Keyword(s):  
Cyclic Amp ◽  
Thyroid Hormones ◽  
Thyroid Function ◽  
Healthy Subjects ◽  
Blood Levels ◽  
Sensitive Test ◽  
Normal Response ◽  
Hypothyroid Patients ◽  
Hyperthyroid Patients

ABSTRACT The glucagon stimulated increase in plasma cyclic AMP has been studied in 17 healthy subjects, in 13 hyperthyroid and in 14 hypothyroid patients. Six hyperthyroid and 2 hypothyroid patients were re-investigated after at least 15 months of treatment. The results show: 1) The glucagon stimulated cyclic AMP response is significantly increased in hyperthyroid patients considered as a group, and is reduced in patients with hypothyroidism. 2) Three hyperthyroid and 4 hypothyroid patients showed a normal response to iv glucagon, indicating that the plasma cyclic AMP response to iv glucagon is not a sensitive test for the evaluation of peripheral thyroid states. This suggests that the effects of thyroid hormones in the liver does not necessarily follow the effects in other tissues. 3) Re-investigation of treated patients showed that the cyclic AMP response can be normalized by treatment, both in hyperthyroidism and in hypothyroidism. However, in patients treated for hyperthyroidism a hyper-response to glucagon can continue after blood levels of thyroid hormones are reduced to normal. This suggests an inertia in the loss of the hyper-response to glucagon, once a hyperfunction has been induced. A similar inertia in the loss of glucagon sensitivity in hypothyroidism could explain the large number of normal tests in hypothyroid patients.

EVALUATION OF THYROID STATUS WITH THE "EFFECTIVE THYROXINE RATIO" (E. T. R.®)

1974 ◽  
Vol 77 (2) ◽  
pp. 250-256 ◽  
Author(s):  
Klaus Kølendorf ◽  
Kaj Siersbæk-Nielsen ◽  
Jens Mølholm Hansen ◽  
Thorkild Friis
Keyword(s):  
Thyroid Function ◽  
Thyroid Function Test ◽  
Free Thyroxine ◽  
Normal Range ◽  
High Positive Correlation ◽  
Function Test ◽  
Free Thyroxine Index ◽  
Hypothyroid Patients ◽  
Hyperthyroid Patients

ABSTRACT A new in vitro thyroid function test, "Effective Thyroxine Ratio" (ETR®) has been tested in 170 patients, and the ETR values in patients with normal and abnormal thyroid function have been compared to the concentration of absolute free thyroxine and free thyroxine index in serum. Among 42 hyperthyroid patients tested with ETR, 13 had values within normal range (30.9 per cent). Three of 18 hypothyroid patients had normal values (16.8 per cent). Eighty-nine per cent of 27 pregnant women and all of 17 patients treated with genuine oestrogens had ETR values within normal range. A high positive correlation was found between values of ETR, absolute free thyroxine in serum (r = 0.62) and free thyroxine index (r = 0.83).

HUMAN LYMPHOCYTE BINDING AND DEIODINATION OF THYROID HORMONES IN RELATION TO THYROID FUNCTION

1975 ◽  
Vol 80 (4) ◽  
pp. 642-656 ◽  
Author(s):  
Ann-Charlotte Holm ◽  
Thérèse Lemarchand-Béraud ◽  
Bianca-Rosa Scazziga ◽  
Serge Cuttelod
Keyword(s):  
Thyroid Hormones ◽  
Thyroid Function ◽  
Human Lymphocyte ◽  
High Serum ◽  
Affinity Constant ◽  
Control Group ◽  
Binding Characteristics ◽  
The Mean ◽  
Hypothyroid Patients ◽  
Hyperthyroid Patients

ABSTRACT The human lymphocyte has been investigated regarding its function as a thyroid hormone target cell. Binding and deiodination of the thyroid hormones were determined after simultaneous incubation of 131I-labelled L-thyroxine (131I-T4) and 125I-labelled L-triiodothyronine (125I-T3) with lymphocytes from healthy subjects, from hyperthyroid and primary hypothyroid patients before and after treatment. The mean percentages of binding, 8.0 ± 0.5 (mean ± sem) for 131I-T4, and 9.7 ± 0.4 for 125I-T3 in the control group, were increased in the hyperthyroids to 10.1 ± 0.4 and 12.7 ± 0.6 respectively, and in the hypothyroids to 10.9 ± 0.7 and 12.8 ± 0.6. All elevated values returned to normal with successful treatment. The mean percentage of deiodination, 12.0 ± 1.7 for 131I-T4, and 6.5 ± 0.9 for 125I-T3 in the control group, showed a threefold increase in the hyperthyroid patients, to 35.9 ± 3.2 and 20.2 ± 1.9 respectively and remained unaltered in the hypothyroid patients. The values of successfully treated hyperthyroid patients were normal and those of the treated hypothyroid patients below normal. Human TSH added to the incubation medium stimulated the binding of both hormones, without influencing deiodination. Thus TSH might be active at the peripheral cellular level. This could contribute to the explanation of the increased binding by lymphocytes from primary hypothyroid patients with high serum concentrations of TSH. A preliminary analysis of the binding characteristics revealed an equilibrium affinity constant of 1.03·1010 m−1 for T3, and of 3.97· 109 m−1 for T4, with corresponding total numbers of binding sites of 1500 and 2000 per cell. It is concluded that, since lymphocyte activities closely reflect the thyroid function, these cells are well suited for studies on the peripheral fate of thyroid hormones and their cellular receptors.

Serum bile acid profile in thyroid dysfunction and effect of medical treatment

1987 ◽  
Vol 73 (4) ◽  
pp. 425-429 ◽  
Author(s):  
Tomoo Kosuge ◽  
Tomoe Beppu ◽  
Takao Kodama ◽  
Koh Hidai ◽  
Yasuo Idezuki
Keyword(s):  
Bile Acid ◽  
Thyroid Hormone ◽  
Bile Acids ◽  
Cholic Acid ◽  
Thyroid Function ◽  
Chenodeoxycholic Acid ◽  
Thyroid Dysfunction ◽  
Serum Bile Acid ◽  
Hypothyroid Patients ◽  
Hyperthyroid Patients

1. Serum non-esterified bile acid profile was examined in patients with thyroid dysfunction. Sixteen hyperthyroid patients, six hypothyroid patients, nine patients taking thyroid or antithyroid drugs and 26 healthy controls were studied. The medicated patients were euthyroid when serum samples were collected. Bile acid concentration was determined by the simplified microassay method involving mass fragmentation spectrometry. 2. The sum of the concentrations of the individual bile acids was not significantly different among the four groups. However, the composition of bile acid reflected the thyroid function. The most prominent bile acid was deoxycholic acid in the hypothyroid patients and chenodeoxycholic acid in the hyperthyroid patients. The serum bile acid profile of medically treated patients was similar to that of normal cpntrols. The ratio of the sum of deoxycholic and cholic acid to that of lithocholic and chenodeoxycholic acid was found to be a good indicator of thyroid function, while the ratio of cholic acid to chenodeoxycholic acid correlated poorly with it. 3. The characteristic effect of thyroid hormone on the serum bile acid composition in man was the shift from the ‘family’ of cholic acid to that of chenodeoxycholic acid. This is in agreement with experimental results in the rat, and suggests a specific action of thyroid hormone on the hydroxylating enzymes involved in the conversion of cholesterol into bile acids.

The circadian rhythm of melatonin in hypothyroidism and hyperthyroidism

1988 ◽  
Vol 119 (2) ◽  
pp. 240-244 ◽  
Author(s):  
Piotr Soszyński ◽  
Stefan Zgliczyński ◽  
Jolanta Pucilowska
Keyword(s):  
Circadian Rhythm ◽  
Pineal Gland ◽  
Thyroid Function ◽  
Thyroid Disorders ◽  
Healthy Controls ◽  
Melatonin Secretion ◽  
Melatonin Concentration ◽  
Hypothyroid Patients ◽  
Hyperthyroid Patients ◽  
Tsh Levels

Abstract. In order to determine the influence of thyroid function on the pineal gland in humans, the circadian rhythm of the serum melatonin concentration was estimated in 16 women with thyroid disorders: 8 with hypothyroidism and 8 with hyperthyroidism, as well as in 5 healthy controls. A significant melatonin circadian rhythm was observed in all the three groups studied. The melatonin rhythm parameters derived from cosinor analysis: mesor (controls: 0.163 ± 0.03 nmol/l (± sem), hypothyroid patients: 0.176 ± 0.22 nmol/l, and hyperthyroid patients: 0.167 ± 0.04 nmol), amplitude (0.155, 0.145 and 0.138 nmol/l, respectively), acrophase (1:38, 2:22 and 1:51 h, respectively) did not differ significantly in the three groups studied. Integrated 24-h melatonin secretion was also similar in patients and controls. The melatonin concentrations were positively correlated with TSH levels in hypothyroidism, and negatively correlated with T3 in hyperthyroidism. In conclusion: in patients with hypo- and hyperthyroidism the circadian rhythm of melatonin secretion is not altered.

THE EFFECT OF NUTRITIONAL STATUS AND THYROID FUNCTION ON THE METABOLISM OF OESTRADIOL

1965 ◽  
Vol 32 (1) ◽  
pp. 107-115 ◽  
Author(s):  
J. B. BROWN ◽  
J. A. STRONG
Keyword(s):  
Nutritional Status ◽  
Urinary Excretion ◽  
Thyroid Function ◽  
Thyroid Disease ◽  
Lower Percentage ◽  
Obese Patients ◽  
Hypothyroid Patients ◽  
Hyperthyroid Patients

SUMMARY The urinary excretion of endogenous and exogenous oestrogens was studied in patients with disordered thyroid function, and in obese and non-obese patients without evident thyroid disease. Hypothyroid patients and obese patients converted a larger percentage of administered oestradiol-17β to urinary oestriol and a lower percentage to urinary oestrone than hyperthyroid patients and non-obese patients. The recovery of administered oestradiol as oestriol, oestrone and oestradiol in urine was the same in the hypothyroid, obese and non-obese patients but considerably lower in the hyperthyroid patients. Similar differences were found in the urinary excretion of endogenous oestrogens. The excretion of endogenous oestrogens varies with the weight of the patient; it was greater in the obese than in the non-obese. The findings indicate that the pathways of oestrogen metabolism are influenced by a number of the factors, including thyroid function, associated with the weight of the patient.

LABORATORY TESTS OF THYROID FUNCTION IN HYPERTHYROIDISM II.

1969 ◽  
Vol 62 (4_Suppla) ◽  
pp. S23-S35
Author(s):  
B.-A. Lamberg ◽  
O. P. Heinonen ◽  
K. Liewendahl ◽  
G. Kvist ◽  
M. Viherkoski ◽  
...  
Keyword(s):  
Thyroid Function ◽  
Laboratory Tests ◽  
Goodness Of Fit ◽  
Point Of View ◽  
Accurate Estimation ◽  
Diagnostic Efficiency ◽  
Normal Limits ◽  
Significant Difference ◽  
Crossing Point ◽  
Hyperthyroid Patients

ABSTRACT The distributions of 13 variables based on 10 laboratory tests measuring thyroid function were studied in euthyroid controls and in patients with toxic diffuse or toxic multinodular goitre. Density functions were fitted to the empirical data and the goodness of fit was evaluated by the use of the χ2-test. In a few instances there was a significant difference but the material available was in some respects too small to allow a very accurate estimation. The normal limits for each variable was defined by the 2.5 and 97.5 percentiles. It appears that in some instances these limits are too rigorous from the practical point of view. It is emphasized that the crossing point of the functions for euthyroid controls and hyperthyroid patients may be a better limit to use. In a preliminary analysis of the diagnostic efficiency the variables of total or free hormone concentration in the blood proved clearily superior to all other variables.

Compensated 131I-therapy of solitary autonomous thyroid nodules: effect on thyroid size and early hypothyroidism

1986 ◽  
Vol 113 (2) ◽  
pp. 226-232 ◽  
Author(s):  
Laszlo Hegedüs ◽  
Dagmar Veiergang ◽  
Steen Karstrup ◽  
Jens Mølholm Hansen
Keyword(s):  
Thyroid Gland ◽  
Thyroid Function ◽  
Thyroid Nodules ◽  
Thyroid Volume ◽  
131I Therapy ◽  
Important Place ◽  
Gland Volume ◽  
Thyroid Gland Volume ◽  
One Year ◽  
Hyperthyroid Patients

Abstract. Thyroid function and thyroid gland volume, ultrasonically determined, were investigated in 27 hyperthyroid patients with solitary autonomous thyroid nodules before and during one year after 131I-treatment. Total thyroid volume decreased gradually from 40.9 ± 3.5 ml (mean ± sem) before treatment to 23.9 ± 1.8 ml (P < 0.001) at 3 months after 131I-treatment. No further change was observed. All but two patients received only one dose of 131I, and in spite of a significant decrease also of the non-adenoma side of the gland, none became hypothyroid. We conclude that 131I-therapy has an important place in the treatment of solitary autonomous thyroid nodules since all our patients became euthyroid within 3 months, only 2 of 27 patients needed more than one dose of 131I, no cases of hypothyroidism occurred, and thyroid volume was substantially decreased.

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