scholarly journals Construction of gold-siRNANPR1 nanoparticles for effective and quick silencing of NPR1 in Arabidopsis thaliana

RSC Advances ◽  
2020 ◽  
Vol 10 (33) ◽  
pp. 19300-19308
Author(s):  
Wen-Xue Lei ◽  
Zi-Shuai An ◽  
Bai-Hong Zhang ◽  
Qian Wu ◽  
Wen-Jun Gong ◽  
...  
Keyword(s):  
Gold Nanoparticles ◽  
Arabidopsis Thaliana ◽  
Gene Silencing ◽  
Transfection Efficiency ◽  
High Transfection Efficiency

Gold nanoparticles (AuNPs) have been widely used as gene silencing agents and therapeutics for treatment due to their high transfection efficiency and lack of cytotoxicity, but their roles in gene silencing in plants have not yet been reported.

Defined pH-sensitive nanogels as gene delivery platform for siRNA mediated in vitro gene silencing

2017 ◽  
Vol 5 (11) ◽  
pp. 2328-2336 ◽  
Author(s):  
Mathias Dimde ◽  
Falko Neumann ◽  
Felix Reisbeck ◽  
Svenja Ehrmann ◽  
Jose Luis Cuellar-Camacho ◽  
...  
Keyword(s):  
Gene Delivery ◽  
Gene Silencing ◽  
Transfection Efficiency ◽  
Genetic Material ◽  
Ph Sensitive ◽  
Carrier System ◽  
High Transfection Efficiency ◽  
Delivery Platform ◽  
Low Cytotoxicity

An advanced cationic carrier system which combines high transfection efficiency with low cytotoxicity and a control over the release of the encapsulated genetic material by the reduction of the multivalent architecture upon pH triggered degradation was developed.

scholarly journals Surface-Initiated Atom Transfer Polymerized Anionic Corona on Gold Nanoparticles for Anti-Cancer Therapy

Pharmaceutics ◽  
2020 ◽  
Vol 12 (3) ◽  
pp. 261
Author(s):  
Wei Mao ◽  
Sol Lee ◽  
Ji Un Shin ◽  
Hyuk Sang Yoo
Keyword(s):  
Gold Nanoparticles ◽  
Transfection Efficiency ◽  
Cancer Therapeutics ◽  
Atom Transfer ◽  
Layer By Layer ◽  
Dependent Manner ◽  
Metallic Particles ◽  
Anti Cancer

Surface initiated atom transfer radical polymerization (SI-ATRP) documented a simple but efficient technique to grow a dense polymer layer on any surface. Gold nanoparticles (AuNPs) give a broad surface to immobilize sulfhyryl group-containing initiators for SI-ATRP; in addition, AuNPs are the major nanoparticulate carriers for delivery of anti-cancer therapeutics, since they are biocompatible and bioinert. In this work, AuNPs with a disulfide initiator were polymerized with sulfoethyl methacrylate by SI-ATRP to decorate the particles with anionic corona, and branched polyethyeleneimine (PEI) and siRNA were sequentially layered onto the anionic corona of AuNP by electrostatic interaction. The in vitro anti-cancer effect confirmed that AuNP with anionic corona showed higher degrees of apoptosis as well as suppression of the oncogene expression in a siRNA dose-dependent manner. The in vivo study of tumor-bearing nude mice revealed that mice treated with c-Myc siRNA-incorporated AuNPs showed dramatically decreased tumor size in comparison to those with free siRNA for 4 weeks. Furthermore, histological examination and gene expression study revealed that the decorated AuNP significantly suppressed c-Myc expression. Thus, we envision that the layer-by-layer assembly on the anionic brushes can be potentially used to incorporate nucleic acids onto metallic particles with high transfection efficiency.

Smuggling gold nanoparticles across cell types – A new role for exosomes in gene silencing

2017 ◽  
Vol 13 (4) ◽  
pp. 1389-1398 ◽  
Author(s):  
Catarina Roma-Rodrigues ◽  
Francisca Pereira ◽  
António P. Alves de Matos ◽  
Marta Fernandes ◽  
Pedro V. Baptista ◽  
...  
Keyword(s):  
Gold Nanoparticles ◽  
Gene Silencing ◽  
Cell Types

High Transfection Efficiency of Cationic Lipids with Asymmetric Acyl–Cholesteryl Hydrophobic Tails

2011 ◽  
Vol 17 (11) ◽  
pp. 3287-3295 ◽  
Author(s):  
Widchaya Radchatawedchakoon ◽  
Aungkana Krajarng ◽  
Nattisa Niyomtham ◽  
Ramida Watanapokasin ◽  
Boon‐ek Yingyongnarongkul
Keyword(s):  
Transfection Efficiency ◽  
Cationic Lipids ◽  
High Transfection Efficiency

Serum Compatible Spermine‐based Cationic Lipids with Non‐identical Hydrocarbon Tails Mediate High Transfection Efficiency

ChemBioChem ◽  
2022 ◽  
Author(s):  
Chopaka Thongbamrer ◽  
Wanlapa Roobsoong ◽  
Jetsumon Sattabongkot ◽  
Praneet Opanasopit ◽  
Boon-ek Yingyongnarongkul
Keyword(s):  
Transfection Efficiency ◽  
Cationic Lipids ◽  
High Transfection Efficiency

Biodegradable nano-organosilica gene carrier for high-efficiency gene transfection

2020 ◽  
Vol 8 (12) ◽  
pp. 2483-2494
Author(s):  
Kun Zeng ◽  
Li Ma ◽  
Wenxiu Yang ◽  
Shan Lei ◽  
Mozhen Wang ◽  
...  
Keyword(s):  
High Efficiency ◽  
Transfection Efficiency ◽  
Gene Transfection ◽  
Gene Carrier ◽  
Gene Vector ◽  
High Transfection Efficiency ◽  
In Cells

Guanidinated-fluorinated α-polylysine-modified organosilica nanoparticles can form a novel raisin-bread-like gene vector, which is disintegrated in cells by GSH to show high transfection efficiency.

Green Synthesis of Gold Nanoparticles using Arabidopsis thaliana and Dunaliella salina Cell Cultures

2018 ◽  
Vol 13 (9-10) ◽  
pp. 539-545 ◽  
Author(s):  
D. S. Chumakov ◽  
A. O. Sokolov ◽  
V. A. Bogatyrev ◽  
O. I. Sokolov ◽  
N. Yu. Selivanov ◽  
...  
Keyword(s):  
Gold Nanoparticles ◽  
Arabidopsis Thaliana ◽  
Green Synthesis ◽  
Cell Cultures ◽  
Dunaliella Salina ◽  
Salina Cell

scholarly journals Comparison between Lipofectamine RNAiMAX and GenMute transfection agents in two cellular models of human hepatoma

2019 ◽  
Vol 63 (3) ◽  
Author(s):  
Clarissa Berardo ◽  
Veronica Siciliano ◽  
Laura G. Di Pasqua ◽  
Plinio Richelmi ◽  
Mariapia Vairetti ◽  
...  
Keyword(s):  
Gene Silencing ◽  
Transfection Efficiency ◽  
Negative Control ◽  
Human Hepatoma ◽  
Cellular Models ◽  
Gapdh Mrna ◽  
Powerful Approach ◽  
Hepatic Cell Lines ◽  
Transfection Agent ◽  
Hepatoma Hepg2

RNA interference is a powerful approach to understand gene function both for therapeutic and experimental purposes. Since the lack of knowledge in the gene silencing of various hepatic cell lines, this work was aimed to compare two transfection agents, the liposome-based Lipofectamine™ RNAiMAX and the HepG2-specific, polymer-based GenMute™, in two cellular models of human hepatoma, HepG2 and Huh7.5. In the first part, we assessed transfection efficiency of a fluorescent Cy3-labeled negative control siRNA by cell imaging analysis; we found that cells treated with GenMute present a higher uptake of the fluorescent negative control siRNA when compared to Lipofectamine RNAiMAX-transfected cells, both in HepG2 and in Huh7.5 cells. In the second part, we evaluated GAPDH silencing with the two transfection reagents by RT-PCR similar GAPDH mRNA expression after each transfection treatment. Finally, we measured cell viability by the MTT assay, observing that cells transfected with GenMute have higher viability with respect to Lipofectamine RNAiMAX-administered cells. These results suggest that GenMute reagent might be considered the most suitable transfection agent for hepatic gene silencing.

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