Biodegradable nano-organosilica gene carrier for high-efficiency gene transfection

A novel polycationic gene carrier was prepared by conjugation of low molecular weight polyethyleneimine with gelatin through 4-bromonaphthaleic anhydride with exceptionally high transfection efficiency.

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α,β-Poly(l-aspartate-graft-PEI): A pseudo-branched PEI as a gene carrier with low toxicity and high transfection efficiency

Acta Biomaterialia ◽

10.1016/j.actbio.2009.03.012 ◽

2009 ◽

Vol 5 (7) ◽

pp. 2485-2494 ◽

Cited By ~ 26

Author(s):

Jia-Hui Yu ◽

Ji-Shan Quan ◽

Jin Huang ◽

Cheng-Yun Wang ◽

Bo Sun ◽

...

Keyword(s):

Transfection Efficiency ◽

Gene Carrier ◽

High Transfection Efficiency ◽

Low Toxicity

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Cardiac-targeting magnetic lipoplex delivery of SH-IGF1R plasmid attenuate norepinephrine-induced cardiac hypertrophy in murine heart

Bioscience Reports ◽

10.1042/bsr20130107 ◽

2014 ◽

Vol 34 (5) ◽

Cited By ~ 1

Author(s):

Yiping Xu ◽

Xuebiao Li ◽

Minjian Kong ◽

Daming Jiang ◽

Aiqiang Dong ◽

...

Keyword(s):

Cardiac Hypertrophy ◽

Molecular Mechanisms ◽

High Efficiency ◽

Transfection Efficiency ◽

Gene Transfection ◽

Pressure Overload ◽

Posterior Wall ◽

Promising Method ◽

Heart Weight

Recent studies have demonstrated a number of molecular mechanisms contributing to the initiation of cardiac hypertrophy response to pressure overload. IGF1R (insulin-like growth factor-1 receptor), an important oncogene, is overexpressed in hypertrophic heart and mediates the hypertrophic pathology process. In this study, we applied with liposomal magnetofection that potentiated gene transfection by applying an external magnetic field to enhance its transfection efficiency. Liposomal magnetofection provided high efficiency in transgene expression in vivo. In vivo, IGF1R-specific-shRNA (small-hairpin RNA) by magnetofection inhibited IGF1R protein expression by 72.2±6.8, 80.7±9.6 and 84.5±5.6%, at 24, 48 and 72 h, respectively, after pGFPshIGF1R injection, indicating that liposomal magnetofection is a promising method that allows the targeting of gene therapy for heart failure. Furthermore, we found that the treated animals (liposomal magnetofection with shIGF1R) showed reduced septal and posterior wall thickness, reduced HW:BWs (heart weight-to-body weights) compared with controls. Moreover, we also found that liposomal magnetofection-based shIGF1R transfection decreased the expression level of p-ERK (phosphorylated extracellular-signal-regulated kinase)1/2, p-AKT1 (phosphorylated protein kinase B1) compared with untreated hearts. These results suggested that liposomal magnetofection-mediated IGF1R-specific-shRNA may be a promising method, and suppression the IGF1R expression inhibited norepinephrine-induced cardiac hypertrophic process via inhibiting PI3K (phosphoinositide 3-kinase)/AKT pathway.

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Gene Transfection of Human Turbinate Mesenchymal Stromal Cells Derived from Human Inferior Turbinate Tissues

Stem Cells International ◽

10.1155/2016/4735264 ◽

2016 ◽

Vol 2016 ◽

pp. 1-10

Author(s):

Jin Seon Kwon ◽

Seung Hun Park ◽

Ji Hye Baek ◽

Truong Minh Dung ◽

Sung Won Kim ◽

...

Keyword(s):

Gene Therapy ◽

Mesenchymal Stromal Cells ◽

Stromal Cells ◽

High Efficiency ◽

Transfection Efficiency ◽

Gene Transfection ◽

Inferior Turbinate ◽

Nonviral Gene Delivery ◽

Charge Ratio ◽

Pei Nanoparticles

Human turbinate mesenchymal stromal cells (hTMSCs) are novel stem cells derived from nasal inferior turbinate tissues. They are easy to isolate from the donated tissue after turbinectomy or conchotomy. In this study, we applied hTMSCs to a nonviral gene delivery system using polyethyleneimine (PEI) as a gene carrier; furthermore, the cytotoxicity and transfection efficiency of hTMSCs were evaluated to confirm their potential as resources in gene therapy. DNA-PEI nanoparticles (NPs) were generated by adding the PEI solution to DNA and were characterized by a gel electrophoresis and by measuring particle size and surface charge of NPs. The hTMSCs were treated with DNA-PEI NPs for 4 h, and toxicity of NPs to hTMSCs and gene transfection efficiency were monitored using MTT assay, fluorescence images, and flow cytometry after 24 h and 48 h. At a high negative-to-positive charge ratio, DNA-PEI NPs treatment led to cytotoxicity of hTMSCs, but the transfection efficiency of DNA was increased due to the electrostatic effect between the NPs and the membranes of hTMSCs. Importantly, the results of this research verified that PEI could deliver DNA into hTMSCs with high efficiency, suggesting that hTMSCs could be considered as untapped resources for applications in gene therapy.

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Efficient gene transfection to liver cells via the cellular regulation of a multifunctional polylactitol-based gene transporter

Journal of Materials Chemistry B ◽

10.1039/c5tb01799h ◽

2016 ◽

Vol 4 (12) ◽

pp. 2208-2218 ◽

Cited By ~ 4

Author(s):

Young-Dong Kim ◽

Tae-Eun Park ◽

Bijay Singh ◽

Kye-Soo Cho ◽

Jaiprakash N. Sangshetti ◽

...

Keyword(s):

Liver Cell ◽

Transfection Efficiency ◽

Gene Transfection ◽

Gene Carrier ◽

Cell Targeting ◽

Cellular Regulation ◽

Efficient Gene ◽

Low Cytotoxicity

A new polylactitol-based multifunctional gene carrier has shown low cytotoxicity, a high transfection efficiency, and liver cell targeting bothin vitroandin vivo.

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Novel Thermoresponsive Nonviral Gene Vector: P(NIPAAm-co-NDAPM)-b-PEI with Adjustable Gene Transfection Efficiency

Bioconjugate Chemistry ◽

10.1021/bc700478s ◽

2008 ◽

Vol 19 (7) ◽

pp. 1368-1374 ◽

Cited By ~ 28

Author(s):

Han Cheng ◽

Jing-Ling Zhu ◽

Yun-Xia Sun ◽

Si-Xue Cheng ◽

Xian-Zheng Zhang ◽

...

Keyword(s):

Transfection Efficiency ◽

Gene Transfection ◽

Gene Vector

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Fabrication and Biological Activities of Plasmid DNA Gene Carrier Nanoparticles Based on Biodegradable l-Tyrosine Polyurethane

Pharmaceuticals ◽

10.3390/ph15010017 ◽

2021 ◽

Vol 15 (1) ◽

pp. 17

Author(s):

Soo-Yong Park ◽

Yang H. Yun ◽

Bum-Joon Park ◽

Hyung-Il Seo ◽

Ildoo Chung

Keyword(s):

Gene Therapy ◽

Cellular Uptake ◽

Plasmid Dna ◽

Transfection Efficiency ◽

Biological Activities ◽

Average Diameter ◽

Gene Carrier ◽

Suitable Alternative ◽

High Transfection Efficiency ◽

Gene Carriers

Gene therapy is a suitable alternative to chemotherapy due to the complications of drug resistance and toxicity of drugs, and is also known to reduce the occurrence of cellular mutation through the use of gene carriers. In this study, gene carrier nanoparticles with minimal toxicity and high transfection efficiency were fabricated from a biocompatible and biodegradable polymer, l-tyrosine polyurethane (LTU), which was polymerized from presynthesized desaminotyrosyl tyrosine hexyl ester (DTH) and polyethylene glycol (PEG), by using double emulsion and solvent evaporation techniques, resulting in the formation of porous nanoparticles, and then used to evaluate their potential biological activities through molecular controlled release and transfection studies. To assess cellular uptake and transfection efficiency, two model drugs, fluorescently labeled bovine serum albumin (FITC-BSA) and plasmid DNA-linear polyethylenimine (LPEI) complex, were successfully encapsulated in nanoparticles, and their transfection properties and cytotoxicities were evaluated in LX2 as a normal cell and in HepG2 and MCF7 as cancer cells. The morphology and average diameter of the LTU nanoparticles were confirmed using light microscopy, transmission electron microscopy, and dynamic light scattering, while confocal microscopy was used to validate the cellular uptake of FITC-BSA-encapsulated LTU nanoparticles. Moreover, the successful cellular uptake of LTU nanoparticles encapsulated with pDNA-LPEI and the high transfection efficiency, confirmed by gel electrophoresis and X-gal assay transfection, indicated that LTU nanoparticles had excellent cell adsorption ability, facilitated gene encapsulation, and showed the sustained release tendency of genes through transfection experiments, with an optimal concentration ratio of pDNA and LPEI of 1:10. All the above characteristics are ideal for gene carriers designed to transport and release drugs into the cytoplasm, thus facilitating effective gene therapy.

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One-Step Formation of Chondrocytes through Direct Reprogramming via Polysaccharide-Based Gene Delivery

Advances in Polymer Technology ◽

10.1155/2019/7632873 ◽

2019 ◽

Vol 2019 ◽

pp. 1-12 ◽

Cited By ~ 2

Author(s):

Qingtong Yu ◽

Yan Wang ◽

Xia Cao ◽

Wenwen Deng ◽

Michael Adu Frimpong ◽

...

Keyword(s):

Gene Delivery ◽

Western Blot ◽

Toluidine Blue ◽

Transfection Efficiency ◽

Gene Transfection ◽

Single Gene ◽

Porphyra Yezoensis ◽

Gene Vector ◽

Collagen Ii ◽

Safranin O

An innovative strategy for the generation of chondrocytes was thoroughly studied in this paper. Polyetherimide-modified polysaccharides of Porphyra yezoensis (pmPPY) served as a nonviral gene vector and delivered Sox9 plasmid to directly reprogram mouse embryonic fibroblasts into chondrocytes. The gene transfer efficiency was evaluated through ELISA, RT-PCR, and Western blot. The induced chondrocytes were identified through toluidine blue, Safranin O, and the immunostaining. The expression level of collagen II was finally evaluated through western blot. The pSox9/pmPPY nanoparticles (1:50) showed lower cytotoxicity as well as greater gene transfection efficiency than Lipofectamine 2000 and polyetherimide (PEI) (p<0.05). The results of toluidine blue, Safranin O, and the immunostaining of collagen II further showed that the normal MEFs were successfully reprogrammed into chondrocytes. These findings indicate that pmPPY could be a promising gene vector for the generation of chondrocytes via single-gene delivery strategy, which might provide abundant chondrocytes for cartilage repair.

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Surface Modification of Gelatin Nanoparticles with Polyethylenimine as Gene Vector

Journal of Nanomaterials ◽

10.1155/2011/646538 ◽

2011 ◽

Vol 2011 ◽

pp. 1-5 ◽

Cited By ~ 11

Author(s):

Wei-Ti Kuo ◽

Hong-Yi Huang ◽

Min-Ju Chou ◽

Meng-Chao Wu ◽

Yi-You Huang

Keyword(s):

Gene Therapy ◽

Surface Modification ◽

Transfection Efficiency ◽

Stable Complex ◽

Gene Vector ◽

Cell Toxicity ◽

Gelatin Nanoparticles ◽

Buffering Effect ◽

High Transfection Efficiency ◽

Pei Nanoparticles

A novel carrier on balancing the transfection efficiency and minimizing cytotoxicity was designed. Gelatin cross-linked with 1.8 kDa of PEI (GA-PEI 1.8 k) formed stable complex and resulted in high positiveζpotential (42.47 mV) and buffering effect. These nanoparticles with N/P ratio of 30 give high transfection efficiency RLU/μg protein and cell viability (86.4%). These modified GA-PEI nanoparticles, with high transfection efficiency and low cell toxicity, can be a potential gene vector in gene therapy.

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