scholarly journals Visible-light triggered templated ligation on surface using furan-modified PNAs

2020 ◽  
Vol 11 (43) ◽  
pp. 11729-11739 ◽  
Author(s):  
Alex Manicardi ◽  
Enrico Cadoni ◽  
Annemieke Madder
Keyword(s):  
Surface Modification ◽  
Visible Light ◽  
Singlet Oxygen ◽  
Furan Moiety

Pinpoint production of singlet oxygen was exploited for a self-contained light-triggered activation of a pro-reactive furan moiety, allowing selective and templated surface modification by recognition of short 22mer oligonucleotides.

scholarly journals Visible-Light Triggered Templated Ligation on Surface Using Furan-Modified PNAs

2020 ◽  
Author(s):  
Alex Manicardi ◽  
Enrico Cadoni ◽  
Annemieke Madder
Keyword(s):  
Nucleic Acid ◽  
Visible Light ◽  
Singlet Oxygen ◽  
Target Detection ◽  
High Throughput ◽  
White Light ◽  
Peptide Nucleic Acids ◽  
Photo Oxidation ◽  
Reducing Conditions ◽  
Furan Moiety

Oligonucleotide-templated reactions are frequently exploited for target detection in biosensors and for the construction of DNA-based materials and probes in nanotechnology. Translation of the specifically used template chemistry from solution to surfaces, with the final aim of achieving highly selective high-throughput systems, has been difficult to reach and poorly explored. Here, we show the first example of a visible light-triggered templated ligation on a surface, employing furan-modified peptide nucleic acids (PNAs). Tailored photo-oxidation of the pro-reactive furan moiety is ensured by the simultaneous introduction of a weak photosensitizer as well as a nucleophilic moiety in the reacting PNA strand. This allows a localized production of singlet oxygen for furan activation, which is not affected by probe dilution or reducing conditions. White light irradiation in combination with recognition of a short 22mer oligo sequence that functions as a template, allows sensitive detection of nucleic acid targets in a 96 well plate format.

scholarly journals Visible-Light Triggered Templated Ligation on Surface Using Furan-Modified PNAs

2020 ◽  
Author(s):  
Alex Manicardi ◽  
Enrico Cadoni ◽  
Annemieke Madder
Keyword(s):  
Nucleic Acid ◽  
Visible Light ◽  
Singlet Oxygen ◽  
Target Detection ◽  
High Throughput ◽  
White Light ◽  
Peptide Nucleic Acids ◽  
Photo Oxidation ◽  
Reducing Conditions ◽  
Furan Moiety

Oligonucleotide-templated reactions are frequently exploited for target detection in biosensors and for the construction of DNA-based materials and probes in nanotechnology. Translation of the specifically used template chemistry from solution to surfaces, with the final aim of achieving highly selective high-throughput systems, has been difficult to reach and poorly explored. Here, we show the first example of a visible light-triggered templated ligation on a surface, employing furan-modified peptide nucleic acids (PNAs). Tailored photo-oxidation of the pro-reactive furan moiety is ensured by the simultaneous introduction of a weak photosensitizer as well as a nucleophilic moiety in the reacting PNA strand. This allows a localized production of singlet oxygen for furan activation, which is not affected by probe dilution or reducing conditions. White light irradiation in combination with recognition of a short 22mer oligo sequence that functions as a template, allows sensitive detection of nucleic acid targets in a 96 well plate format.

Optical Properties of Photodynamic Therapy Drugs in Different Environments: The Paradigmatic Case of Temoporfin

2020 ◽  
Author(s):  
busenur Aslanoglu ◽  
Ilya Yakavets ◽  
Vladimir Zorin ◽  
Henri-Pierre Lassalle ◽  
Francesca Ingrosso ◽  
...  
Keyword(s):  
Drug Delivery ◽  
Optical Properties ◽  
Photodynamic Therapy ◽  
Minimally Invasive ◽  
Visible Light ◽  
Cancer Treatment ◽  
Singlet Oxygen ◽  
Tumor Cells ◽  
Molecular Oxygen ◽  
Computational Tools

Computational tools have been used to study the photophysical and photochemical features of photosensitizers in photodynamic therapy (PDT) –a minimally invasive, less aggressive alternative for cancer treatment. PDT is mainly based by the activation of molecular oxygen through the action of a photoexcited sensitizer (photosensitizer). Temoporfin, widely known as mTHPC, is a second-generation photosensitizer, which produces the cytotoxic singlet oxygen when irradiated with visible light and hence destroys tumor cells. However, the bioavailability of the mostly hydrophobic photosensitizer, and hence its incorporation into the cells, is fundamental to achieve the desired effect on malignant tissues by PDT. In this study, we focus on the optical properties of the temoporfin chromophore in different environments –in <i>vacuo</i>, in solution, encapsulated in drug delivery agents, namely cyclodextrin, and interacting with a lipid bilayer.

scholarly journals Surface Modification of TaON with Monoclinic ZrO2to Produce a Composite Photocatalyst with Enhanced Hydrogen Evolution Activity under Visible Light

2008 ◽  
Vol 81 (8) ◽  
pp. 927-937 ◽  
Author(s):  
Kazuhiko Maeda ◽  
Hiroaki Terashima ◽  
Kentaro Kase ◽  
Masanobu Higashi ◽  
Masashi Tabata ◽  
...  
Keyword(s):  
Surface Modification ◽  
Visible Light ◽  
Hydrogen Evolution ◽  
Composite Photocatalyst

scholarly journals Facile Preparation of Efficient WO3Photocatalysts Based on Surface Modification

2015 ◽  
Vol 2015 ◽  
pp. 1-7 ◽  
Author(s):  
Min Liu ◽  
Hongmei Li ◽  
Yangsu Zeng
Keyword(s):  
Photocatalytic Activity ◽  
Surface Modification ◽  
Visible Light ◽  
Photoelectron Spectroscopy ◽  
Impregnation Method ◽  
Light Spectra ◽  
Simple Strategy ◽  
Visible Light Active ◽  
Efficient Charge ◽  
Surface Modified

Tungsten trioxide (WO3) was surface modified with Cu(II) nanoclusters and titanium dioxide (TiO2) nanopowders by using a simple impregnation method followed by a physical combining method. The obtained nanocomposites were studied by scanning electron microscope, X-ray photoelectron spectroscopy spectra, UV-visible light spectra, and photoluminescence, respectively. Although the photocatalytic activity of WO3was negligible under visible light irradiation, the visible light photocatalytic activity of WO3was drastically enhanced by surface modification of Cu(II) nanoclusters and TiO2nanopowders. The enhanced photocatalytic activity is due to the efficient charge separation by TiO2and Cu(II) nanoclusters functioning as cocatalysts on the surface. Thus, this simple strategy provides a facile route to prepare efficient visible-light-active photocatalysts for practical application.

Extending the excitation wavelength from UV to visible light for a europium complex-based mitochondria targetable luminescent probe for singlet oxygen

2018 ◽  
Vol 47 (37) ◽  
pp. 12852-12857 ◽  
Author(s):  
Hua Ma ◽  
Xin Wang ◽  
Bo Song ◽  
Liu Wang ◽  
Zhixin Tang ◽  
...  
Keyword(s):  
Visible Light ◽  
Singlet Oxygen ◽  
Europium Complex ◽  
Excitation Wavelength ◽  
Luminescent Probe ◽  
Luminescence Detection

A visible-light-excitable Eu3+ complex-based luminescent probe for the time-gated luminescence detection of singlet oxygen in vitro and in vivo.

scholarly journals Singlet oxygen generation properties of an inclusion complex of cyclic free-base porphyrin dimer and fullerene C60

RSC Advances ◽  
2017 ◽  
Vol 7 (30) ◽  
pp. 18690-18695 ◽  
Author(s):  
Yousuke Ooyama ◽  
Toshiaki Enoki ◽  
Joji Ohshita ◽  
Takuya Kamimura ◽  
Shuwa Ozako ◽  
...  
Keyword(s):  
Inclusion Complex ◽  
Visible Light ◽  
Singlet Oxygen ◽  
Visible Light Irradiation ◽  
Fullerene C60 ◽  
Light Irradiation ◽  
Free Base ◽  
Oxygen Generation ◽  
Singlet Oxygen Generation ◽  
Porphyrin Dimer

We demonstrate that a cyclic free-base porphyrin dimer and its inclusion complex with fullerene C60 possess the ability to generate singlet oxygen (1O2) under visible light irradiation.

Sign in / Sign up

Export Citation Format

Share Document