3D Bioprinting of shear-thinning hybrid bioinks with excellent bioactivity derived from gellan/alginate and thixotropic magnesium phosphate-based gels

2020 ◽  
Vol 8 (25) ◽  
pp. 5500-5514 ◽  
Author(s):  
You Chen ◽  
Xiong Xiong ◽  
Xin Liu ◽  
Rongwei Cui ◽  
Chen Wang ◽  
...  
Keyword(s):  
Sodium Alginate ◽  
Shear Thinning ◽  
Gellan Gum ◽  
Magnesium Phosphate ◽  
Mechanical Support ◽  
3D Bioprinting

A novel shear-thinning hybrid bioink with good printability, mechanical support, biocompatibility, and bioactivity was developed by combining gellan gum, sodium alginate, and thixotropic magnesium phosphate-based gel (GG–SA/TMP-BG).

Shear-Thinning and Thermo-Reversible Nanoengineered Inks for 3D Bioprinting

2017 ◽  
Vol 9 (50) ◽  
pp. 43449-43458 ◽  
Author(s):  
Scott A. Wilson ◽  
Lauren M. Cross ◽  
Charles W. Peak ◽  
Akhilesh K. Gaharwar
Keyword(s):  
Shear Thinning ◽  
3D Bioprinting

The Fabrication of Tissue Engineering Scaffolds by Inkjet Printing Technology

2018 ◽  
Vol 934 ◽  
pp. 129-133 ◽  
Author(s):  
Chao Fan Lv ◽  
Li Ya Zhu ◽  
Jian Ping Shi ◽  
Zong An Li ◽  
Wen Lai Tang ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Sodium Alginate ◽  
Inkjet Printing ◽  
Three Dimensional ◽  
3D Bioprinting ◽  
Tissue Engineering Scaffolds ◽  
Printing Technology ◽  
3D Printed ◽  
Inkjet Printing Technology ◽  
Alginate Scaffold

Three-dimensional (3D) printing has been playing an important role in diverse areas in medicine. In order to promote the development of tissue engineering, this study attempts to fabricate tissue engineering scaffolds using the inkjet printing technology. Sodium alginate, exhibiting similar properties to the native human extracellular matrix (ECM), was used as bioink. The jetted fluid of sodium alginate would be gelatinized when printed into the calcium chloride solution. The characteristics of the 3D-printed sodium alginate scaffold were systematically measured and analyzed. The results show that, the pore size, porosity and degradation property of these scaffolds could be well controlled. This study indicates the capability of 3D bioprinting technology for preparing tissue engineering scaffolds.

scholarly journals Optimization of 3D bioprinting of human neuroblastoma cells using sodium alginate hydrogel

2019 ◽  
Author(s):  
Jakub Lewicki ◽  
Joost Bergman ◽  
Caoimhe Kerins ◽  
Ola Hermanson
Keyword(s):  
Cell Culture ◽  
Cell Viability ◽  
Sodium Alginate ◽  
Neuroblastoma Cell ◽  
3D Culture ◽  
Fine Tuning ◽  
Human Neuroblastoma Cell ◽  
3D Bioprinting ◽  
Human Neuroblastoma

AbstractThere are many parameters in extrusion-based three-dimensional (3D) bioprinting of different materials that require fine-tuning to obtain the optimal print resolution and cell viability. To standardize this process, methods such as parameter optimization index (POI) have been introduced. The POI aims at pinpointing the optimal printing speed and pressure to achieve the highest accuracy keeping theoretical shear stress low. Here we applied the POI to optimize the process of 3D bioprinting human neuroblastoma cell-laden 2% sodium alginate (SA) hydrogel using freeform reversible embedding of suspended hydrogels (FRESH). Our results demonstrate a notable difference between optimal parameters for printing 2% SA with and without cells in the hydrogel. We also detected a significant influence of long-term cell culture on the printed constructs. This observation suggests that the POI has to be evaluated in the perspective of the final application. When taking these conditions into consideration, we could define a set of parameters that resulted in good quality prints maintaining high neuroblastoma cell viability (83% viable cells) during 7 days of cell culture using 2% SA and FRESH bioprinting. These results can be further used to manufacture neuroblastoma in vitro 3D culture systems to be used for cancer research.

scholarly journals A Thermogelling Organic-Inorganic Hybrid Hydrogel with Excellent Printability, Shape Fidelity and Cytocompatibility for 3D Bioprinting

2021 ◽  
Author(s):  
Chen Hu ◽  
Taufiq Ahmad ◽  
Malik Salman Haider ◽  
Lukas Hahn ◽  
Philipp Stahlhut ◽  
...  
Keyword(s):  
Block Copolymer ◽  
Shear Thinning ◽  
3D Bioprinting ◽  
Hybrid Hydrogel ◽  
Inorganic Hybrid

In this study, an advanced hybrid ink was developed, based on a thermogelling block copolymer, alginate and clay. The reversible thermogelling and shear thinning properties polymer acts at the same time as a fugitive material on the macromolecular level and facilitates the cell-laden extrusion based bioprinting. <br>

Nanostructured nickel sulphate: properties and applications in marmalade production

2021 ◽  
pp. 738-745
Author(s):  
A.A. Krolevets ◽  
S.G. Glotova ◽  
E.M. Mamaeva ◽  
K.V. Golubkova
Keyword(s):  
Sodium Alginate ◽  
Functional Food ◽  
Nickel Sulfate ◽  
Spherical Shape ◽  
Food Product ◽  
Gellan Gum ◽  
Nanoparticle Tracking Analysis ◽  
Nickel Sulphate ◽  
Cobalt Sulfate ◽  
Average Size

The paper presents the data on the use of nanostructured nickel sulfate in the production of marmalade as a functional food product for prophylactic purposes. The nanoparticle tracking analysis (NTA method) was used to determine the size of nanostructured nickel sulfate. In this case, the smallest size (25.7 nm) is formed only in gellan gum with a core:shell ratio of 1:1. When the core:shell ratio in gellan gum is 1:3, the average size of nanocapsules is 49.9 nm. This result can be explained by the tighter packing of cobalt sulfate in the capsule. In sodium alginate, the average nanocapsule size is already 191 nm. The polydispersity index in all studied shells and at different core:shell ratios are practically equal and amounts to 0.84–1.09, which corresponds to a spherical shape.

scholarly journals Extracellular Matrix/Amorphous Magnesium Phosphate Bioink for 3D Bioprinting of Craniomaxillofacial Bone Tissue

2020 ◽  
Vol 12 (21) ◽  
pp. 23752-23763 ◽  
Author(s):  
Nileshkumar Dubey ◽  
Jessica A. Ferreira ◽  
Jos Malda ◽  
Sarit B. Bhaduri ◽  
Marco C. Bottino
Keyword(s):  
Extracellular Matrix ◽  
Bone Tissue ◽  
Magnesium Phosphate ◽  
3D Bioprinting

Rheological Properties of Limited Enzymatic Hydrolyzed Wheat Gluten

2012 ◽  
Vol 550-553 ◽  
pp. 1535-1539
Author(s):  
Zhang Cun Wang ◽  
Le Jing Li ◽  
Xue Wei Zhao ◽  
Sheng Wen Cui
Keyword(s):  
Rheological Properties ◽  
Sodium Alginate ◽  
Apparent Viscosity ◽  
Xanthan Gum ◽  
Wheat Gluten ◽  
Shear Thinning ◽  
Effects Of Temperature ◽  
Complex Solutions

The rheological properties of Limited Enzyme-hydrolyzed Wheat Gluten (LHWG) and its complex solutions with xanthan gum (XG), sodium alginate (SA) or gelatin were researched by Brookfield DV-Ⅱ+Pro Viscometer. The results showed that they all exhibited typical shear-thinning behavior and the higher LHWG concentration, the more evident of the shear thinning was. The effects of temperature on the rheology of LHWG were very complicated based on the concentration. The apparent viscosity of 300 mg/mL LHWG (20°C) was higher than that of 100 mg/mL and 200 mg/mL, and showed fluctuation between 20°C and 90°C, which were strongly different from that of low LHWG solution. However, the rheological properties of LHWG were influenced slightly by shearing time.

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