Retroreflection-based sandwich type affinity sensing of isothermal gene amplification product for foodborne pathogen detection

The Analyst ◽  
2022 ◽  
Author(s):  
Danbi Lee ◽  
Eunsuk Kim ◽  
Kyung Won Lee ◽  
Ka Ram Kim ◽  
Hyeong Jin Chun ◽  
...  
Keyword(s):  
Gene Amplification ◽  
Molecular Diagnostics ◽  
Pathogen Detection ◽  
Target Genes ◽  
Foodborne Pathogen ◽  
Isothermal Amplification ◽  
Amplification Product ◽  
Loop Mediated Isothermal Amplification ◽  
Sandwich Type ◽  
Foodborne Pathogen Detection

Loop-mediated isothermal amplification (LAMP) is an outstanding method for molecular diagnostics, as the rapid, specific, and sensitive amplification of target genes is possible. However, it is necessary to measure fluorescence...

A microfluidic lab-on-a-disc integrated loop mediated isothermal amplification for foodborne pathogen detection

2016 ◽  
Vol 227 ◽  
pp. 600-609 ◽  
Author(s):  
Abkar Ahmed Sayad ◽  
Fatimah Ibrahim ◽  
Shah Mukim Uddin ◽  
Koh Xiu Pei ◽  
Mas S. Mohktar ◽  
...  
Keyword(s):  
Pathogen Detection ◽  
Foodborne Pathogen ◽  
Isothermal Amplification ◽  
Loop Mediated Isothermal Amplification ◽  
Foodborne Pathogen Detection

scholarly journals A Portable Automatic Endpoint Detection System for Amplicons of Loop Mediated Isothermal Amplification on Microfluidic Compact Disk Platform

Sensors ◽  
2015 ◽  
Vol 15 (3) ◽  
pp. 5376-5389 ◽  
Author(s):  
Shah Uddin ◽  
Fatimah Ibrahim ◽  
Abkar Sayad ◽  
Aung Thiha ◽  
Koh Pei ◽  
...  
Keyword(s):  
Pathogen Detection ◽  
Detection System ◽  
Liquid Crystal Display ◽  
Foodborne Pathogen ◽  
Isothermal Amplification ◽  
Detection Methods ◽  
Endpoint Detection ◽  
Compact Disk ◽  
Loop Mediated Isothermal Amplification ◽  
The Impact

In recent years, many improvements have been made in foodborne pathogen detection methods to reduce the impact of food contamination. Several rapid methods have been developed with biosensor devices to improve the way of performing pathogen detection. This paper presents an automated endpoint detection system for amplicons generated by loop mediated isothermal amplification (LAMP) on a microfluidic compact disk platform. The developed detection system utilizes a monochromatic ultraviolet (UV) emitter for excitation of fluorescent labeled LAMP amplicons and a color sensor to detect the emitted florescence from target. Then it processes the sensor output and displays the detection results on liquid crystal display (LCD). The sensitivity test has been performed with detection limit up to 2.5 × 10−3 ng/µL with different DNA concentrations of Salmonella bacteria. This system allows a rapid and automatic endpoint detection which could lead to the development of a point-of-care diagnosis device for foodborne pathogens detection in a resource-limited environment.

Loop-mediated isothermal amplification technique: principle, development and wide application in food safety

2020 ◽  
Vol 12 (46) ◽  
pp. 5551-5561
Author(s):  
Tianzeng Huang ◽  
Linzhi Li ◽  
Xing Liu ◽  
Qi Chen ◽  
Xueen Fang ◽  
...  
Keyword(s):  
Food Safety ◽  
Gene Amplification ◽  
Foodborne Pathogens ◽  
High Specificity ◽  
Isothermal Amplification ◽  
Isothermal Conditions ◽  
Loop Mediated Isothermal Amplification ◽  
Novel Gene ◽  
Amplification Method ◽  
Amplification Technique

LAMP is a relatively novel gene amplification method under isothermal conditions with rapidity, and high specificity. It is widely applied in the field of food safety, such as in the detection of foodborne pathogens, GM, OP pesticides and so on

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