scholarly journals Loop-Mediated Isothermal Amplification (LAMP), An Innovation in Gene Amplification: Bridging the Gap in Molecular Diagnostics; A Review

2015 ◽  
Vol 8 (17) ◽  
Author(s):  
Umar Faruk Abdullahi ◽  
Rochman Naim ◽  
Wan Rohani Wan Taib ◽  
Ahmadu Saleh ◽  
Anas Muazu ◽  
...  
Keyword(s):  
Gene Amplification ◽  
Molecular Diagnostics ◽  
Isothermal Amplification ◽  
Loop Mediated Isothermal Amplification

Retroreflection-based sandwich type affinity sensing of isothermal gene amplification product for foodborne pathogen detection

The Analyst ◽  
2022 ◽  
Author(s):  
Danbi Lee ◽  
Eunsuk Kim ◽  
Kyung Won Lee ◽  
Ka Ram Kim ◽  
Hyeong Jin Chun ◽  
...  
Keyword(s):  
Gene Amplification ◽  
Molecular Diagnostics ◽  
Pathogen Detection ◽  
Target Genes ◽  
Foodborne Pathogen ◽  
Isothermal Amplification ◽  
Amplification Product ◽  
Loop Mediated Isothermal Amplification ◽  
Sandwich Type ◽  
Foodborne Pathogen Detection

Loop-mediated isothermal amplification (LAMP) is an outstanding method for molecular diagnostics, as the rapid, specific, and sensitive amplification of target genes is possible. However, it is necessary to measure fluorescence...

Loop-mediated isothermal amplification technique: principle, development and wide application in food safety

2020 ◽  
Vol 12 (46) ◽  
pp. 5551-5561
Author(s):  
Tianzeng Huang ◽  
Linzhi Li ◽  
Xing Liu ◽  
Qi Chen ◽  
Xueen Fang ◽  
...  
Keyword(s):  
Food Safety ◽  
Gene Amplification ◽  
Foodborne Pathogens ◽  
High Specificity ◽  
Isothermal Amplification ◽  
Isothermal Conditions ◽  
Loop Mediated Isothermal Amplification ◽  
Novel Gene ◽  
Amplification Method ◽  
Amplification Technique

LAMP is a relatively novel gene amplification method under isothermal conditions with rapidity, and high specificity. It is widely applied in the field of food safety, such as in the detection of foodborne pathogens, GM, OP pesticides and so on

scholarly journals Evaluation of a Loop-Mediated Isothermal Amplification Assay to Detect Carbapenemases Directly From Bronchoalveolar Lavage Fluid Spiked With Acinetobacter spp.

2021 ◽  
Vol 11 ◽  
Author(s):  
Javier Moreno-Morales ◽  
Andrea Vergara ◽  
Tomislav Kostyanev ◽  
Jesús Rodriguez-Baño ◽  
Herman Goossens ◽  
...  
Keyword(s):  
Bronchoalveolar Lavage ◽  
Molecular Diagnostics ◽  
Bronchoalveolar Lavage Fluid ◽  
Lavage Fluid ◽  
Isothermal Amplification ◽  
Loop Mediated Isothermal Amplification ◽  
Carbapenem Resistant ◽  
Amplification Assay ◽  
Acinetobacter Spp ◽  
Selection Of

Carbapenem-resistant Acinetobacter spp. mainly Acinetobacter baumannii are frequently causing nosocomial infections with high mortality. In this study, the efficacy of the Eazyplex® SuperBug Complete A system, based on loop-mediated isothermal amplification (LAMP), to detect the presence of carbapenemases in Acinetobacter spp. directly from bronchoalveolar lavage (BAL) samples was assessed. A total of 22 Acinetobacter spp. strains producing OXA-23, OXA-40, OXA-58, NDM, and IMP were selected. Eazyplex SuperBug Complete A kit, used with the Genie II device, is a molecular diagnostics kit that detects a selection of genes that express carbapenemases (blaKPC, blaNDM, blaVIM, blaOXA–48, blaOXA–23, blaOXA–40, and blaOXA–58). Negative BAL samples were identified, McFarland solutions were prepared from each of the 22 Acinetobacter strains and serial dilutions in saline solution were made to finally spike BAL samples to a concentration of 102 and 103 CFU/ml. Fifteen concentrations out of the 44 tested out did not provide detection of the carbapenemase-producing gene, all but one being at the lowest concentration tested at 102 CFU/ml; therefore, the limit of sensitivity is 103 CFU/ml. This assay represents the kind of advantages that investing in molecular diagnostics brings to the clinical practice, allowing the identification of carbapenemases in less than 30 min with a sensitivity of 103 CFU/ml.

scholarly journals Progress in loop-mediated isothermal amplification assay for detection of Schistosoma mansoni DNA: towards a ready-to-use test

2019 ◽  
Vol 9 (1) ◽  
Author(s):  
J. García-Bernalt Diego ◽  
P. Fernández-Soto ◽  
B. Crego-Vicente ◽  
S. Alonso-Castrillejo ◽  
B. Febrer-Sendra ◽  
...  
Keyword(s):  
Schistosoma Mansoni ◽  
Molecular Diagnostics ◽  
Neglected Tropical Diseases ◽  
Point Of Care ◽  
Isothermal Amplification ◽  
Diagnostic Tools ◽  
Important Species ◽  
Loop Mediated Isothermal Amplification ◽  
Amplification Assay ◽  
Diagnostic Applications

Abstract Schistosomiasis is one of the most prevalent Neglected Tropical Disease, affecting approximately 250 million people worldwide. Schistosoma mansoni is the most important species causing human intestinal schistosomiasis. Despite significant efforts in recent decades, the global disease burden of schistosomiasis remains extremely high. This could partly be attributed to the absence of accurate diagnostic tools, primarily in endemic areas. Loop-mediated isothermal amplification (LAMP) is increasingly used in molecular diagnostics as a field-friendly alternative to many other complex molecular methods and it has been proposed as an ideal candidate for revolutionizing point-of-care molecular diagnostics. In a previous work, a LAMP-based method to detect S. mansoni DNA (SmMIT-LAMP) was developed by our research group for early diagnosis of active schistosomiasis in an experimental infection murine model. The SmMIT-LAMP has been further successfully evaluated in both human stool and snail samples and, recently, in human urine samples. In this study, we developed an important improvement for SmMIT-LAMP molecular assay, transforming it into a cold maintenance dry format suitable for potentially manufacturing as kit for ready-to-use for schistosomiasis diagnosis. This procedure could be applied to create dry LAMP kits for a laboratory setting and for diagnostic applications for other neglected tropical diseases.

scholarly journals MorphoCatcher: a multiple-alignment based web tool for target selection and designing taxon-specific primers in the loop-mediated isothermal amplification method

PeerJ ◽  
2019 ◽  
Vol 7 ◽  
pp. e6801
Author(s):  
Fedor V. Shirshikov ◽  
Yuri A. Pekov ◽  
Konstantin A. Miroshnikov
Keyword(s):  
Molecular Diagnostics ◽  
Target Selection ◽  
Primer Design ◽  
Multiple Alignment ◽  
Isothermal Amplification ◽  
Specific Primer ◽  
Bioinformatics Tool ◽  
Loop Mediated Isothermal Amplification ◽  
Amplification Method ◽  
Selection Of

Background Advantages of loop-mediated isothermal amplification in molecular diagnostics allow to consider the method as a promising technology of nucleic acid detection in agriculture and medicine. A bioinformatics tool that provides rapid screening and selection of target nucleotide sequences with subsequent taxon-specific primer design toward polymorphic orthologous genes, not only unique or conserved common regions of genome, would contribute to the development of more specific and sensitive diagnostic assays. However, considering features of the original software for primer selection, also known as the PrimerExplorer (Eiken Chemical Co. LTD, Tokyo, Japan), the taxon-specific primer design using multiple sequence alignments of orthologs or even viral genomes with conservative architecture is still complicated. Findings Here, MorphoCatcher is introduced as a fast and simple web plugin for PrimerExplorer with a clear interface. It enables an execution of multiple-alignment based search of taxon-specific mutations, visual screening and selection of target sequences, and easy-to-start specific primer design using the PrimerExplorer software. The combination of MorphoCatcher and PrimerExplorer allows to perform processing of the multiple alignments of orthologs for informative sliding-window plot analysis, which is used to identify the sequence regions with a high density of taxon-specific mutations and cover them by the primer ends for better specificity of amplification. Conclusions We hope that this new bioinformatics tool developed for target selection and taxon-specific primer design, called the MorphoCatcher, will gain more popularity of the loop-mediated isothermal amplification method for molecular diagnostics community. MorphoCatcher is a simple web plugin tool for the PrimerExplorer software which is freely available only for non-commercial and academic users at http://morphocatcher.ru.

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