A fluorescent probe for specifically measuring overall thioredoxin and glutaredoxin reducing activity in bacterial cells

The Analyst ◽  
2022 ◽  
Author(s):  
Xin Zuo ◽  
Ying Zhao ◽  
Jintao Zhao ◽  
Yanfang Ouyang ◽  
Wenjun Qian ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Dna Synthesis ◽  
Fluorescent Probe ◽  
Bacterial Cells ◽  
Cellular Events ◽  
Reducing Activity

The thioredoxins (Trxs) and glutaredoxins (Grxs) are the two major thiol-dependent reductases, participating in many important cellular events such as defense against oxidative stress, DNA synthesis and repair. Both Trxs...

Molecular Dissection of Renal Ischemia-Reperfusion: Oxidative Stress and Cellular Events

2016 ◽  
Vol 23 (19) ◽  
pp. 1965-1980 ◽  
Author(s):  
Branislav Rovcanin ◽  
Branislava Medic ◽  
Gordana Kocic ◽  
Tatjana Cebovic ◽  
Marko Ristic ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Ischemia Reperfusion ◽  
Renal Ischemia ◽  
Molecular Dissection ◽  
Cellular Events

Transient inhibition of DNA synthesis by methotrexate, in the rat embryo and foetus

Development ◽  
1971 ◽  
Vol 26 (3) ◽  
pp. 469-474
Author(s):  
C. L. Berry
Keyword(s):  
Folic Acid ◽  
Dna Synthesis ◽  
Folinic Acid ◽  
Rat Embryo ◽  
Acid Inhibitor ◽  
Cellular Events ◽  
Foetal Life ◽  
Specific Antagonist ◽  
Catch Up ◽  
Inhibition Of Dna Synthesis

Massive doses of methotrexate, a folic acid inhibitor, followed by folinic acid, the specific antagonist, have been used to produce a period in which the embryo and foetus are exposed to tetrahydrofolate deficiency with subsequent inhibition of DNA synthesis. The effects of this inhibition vary at different stages of gestation, and in late foetal life provide a useful method of inducing a delay in the appearance of vertebral body ossification centres. This defect is rapidly repaired, although there may be permanent sequelae. It is hoped that this technique will be useful in the study of cellular events in ‘catch-up’ growth.

A new fluorescent probe with ultralow background fluorescence for imaging of endogenous cellular selenol under oxidative stress

2017 ◽  
Vol 28 (10) ◽  
pp. 1987-1990 ◽  
Author(s):  
Dan Cheng ◽  
Yue Pan ◽  
Bin-Cheng Yin ◽  
Lin Yuan ◽  
Xiao-Bing Zhang
Keyword(s):  
Oxidative Stress ◽  
Fluorescent Probe ◽  
Background Fluorescence

scholarly journals Correlation between DNA synthesis in the second, third and fourth generations of spermatogonia and the occurrence of apoptosis in both spermatogonia and spermatocytes

Reproduction ◽  
2003 ◽  
pp. 661-668 ◽  
Author(s):  
J Blanco-Rodriguez ◽  
C Martinez-Garcia ◽  
A Porras
Keyword(s):  
Cell Cycle ◽  
Dna Replication ◽  
Dna Synthesis ◽  
Functional Significance ◽  
Tunel Assay ◽  
Seminiferous Epithelium ◽  
Cell Cycle Checkpoints ◽  
Clear Correlation ◽  
Cellular Events ◽  
Dna Strand

In the seminiferous epithelium, both DNA synthesis and apoptosis occur at equivalent stages in various species, with apoptosis taking place mainly at the same stages as DNA replication in the second, third and fourth spermatogonial generations. As preservation of the cellular associations found at these stages may have some functional significance, it is important to determine whether there is a correlation between these cellular events. In this study, pairs of immunoperoxidase-stained adjacent testis sections from rats, mice, rabbits and cats in which either bromodeoxyuridine incorporated into the newly synthesized DNA strand (BrdU labelling) or DNA 3' end labelling of the apoptotic DNA fragments (TUNEL assay) were detected were compared. In addition, both events were analysed in double-labelled sections. These two methods revealed a clear correlation between the occurrence of DNA replication in the second to fourth generations of spermatogonia and most physiological apoptosis taking place in both spermatogonia and spermatocytes in the three different mammalian orders (Rodentia, Lagomorpha and Carnivora). This correlation may result from the synchronization of mitotic spermatogonial and meiotic spermatocyte cell cycle checkpoints operating at these stages.

Investigation of endogenous malondialdehyde through fluorescent probe MDA-6 during oxidative stress

2020 ◽  
Vol 1116 ◽  
pp. 9-15 ◽  
Author(s):  
Jing Zhang ◽  
Zihao Yang ◽  
Shanshan Zhang ◽  
Zhenzhen Xie ◽  
Shichao Han ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Fluorescent Probe

scholarly journals Phenolic Compounds Reduce the Fat Content in Caenorhabditis elegans by Affecting Lipogenesis, Lipolysis, and Different Stress Responses

2020 ◽  
Vol 13 (11) ◽  
pp. 355
Author(s):  
Paula Aranaz ◽  
David Navarro-Herrera ◽  
María Zabala ◽  
Ana Romo-Hualde ◽  
Miguel López-Yoldi ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Fatty Acid ◽  
Phenolic Compounds ◽  
Fatty Acid Metabolism ◽  
Acid Metabolism ◽  
Lipid Mobilization ◽  
C Elegans ◽  
Unfolded Protein ◽  
Reducing Activity ◽  
Protein Response

Supplementation with bioactive compounds capable of regulating energy homeostasis is a promising strategy to manage obesity. Here, we have screened the ability of different phenolic compounds (myricetin, kaempferol, naringin, hesperidin, apigenin, luteolin, resveratrol, curcumin, and epicatechin) and phenolic acids (p-coumaric, ellagic, ferulic, gallic, and vanillic acids) regulating C. elegans fat accumulation. Resveratrol exhibited the strongest lipid-reducing activity, which was accompanied by the improvement of lifespan, oxidative stress, and aging, without affecting worm development. Whole-genome expression microarrays demonstrated that resveratrol affected fat mobilization, fatty acid metabolism, and unfolded protein response of the endoplasmic reticulum (UPRER), mimicking the response to calorie restriction. Apigenin induced the oxidative stress response and lipid mobilization, while vanillic acid affected the unfolded-protein response in ER. In summary, our data demonstrates that phenolic compounds exert a lipid-reducing activity in C. elegans through different biological processes and signaling pathways, including those related with lipid mobilization and fatty acid metabolism, oxidative stress, aging, and UPR-ER response. These findings open the door to the possibility of combining them in order to achieve complementary activity against obesity-related disorders.

Rearrangement regulated cysteine fluorescent probe for cellular oxidative stress evaluation induced by copper(II)

2020 ◽  
Vol 31 (11) ◽  
pp. 2970-2974 ◽  
Author(s):  
Na Zhou ◽  
Fangjun Huo ◽  
Yongkang Yue ◽  
Kaiqing Ma ◽  
Caixia Yin
Keyword(s):  
Oxidative Stress ◽  
Fluorescent Probe ◽  
Stress Evaluation

1,3,5‐Triphenylpyrazoline Based Fluorescent Probe for Selective Sensing and Imaging of Glutathione in Live Cell under Oxidative Stress

2020 ◽  
Vol 5 (2) ◽  
pp. 515-521 ◽  
Author(s):  
Siva Bala Subramaniyan ◽  
Sesuraj Babiola Annes ◽  
Manokaran Yuvasri ◽  
Kolanchinathan Nivedha ◽  
Subburethinam Ramesh ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Fluorescent Probe ◽  
Live Cell
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