Ultrasensitive multiplexed miRNA detection based on self-priming hairpin-triggered isothermal cascade reaction

2022 ◽  
Author(s):  
Jayeon Song ◽  
Chang Yeol Lee ◽  
Hyun Gyu Park

We herein describe an ultrasensitive isothermal strategy to detect miRNAs in a multiplexed manner by utilizing self-priming hairpin-triggered cascade reaction and adsorption property of graphene oxide (GO). In principle, the...

2018 ◽  
Vol 37 (9-10) ◽  
pp. 764-776 ◽  
Author(s):  
YL Qin ◽  
WW Zhao ◽  
Z Sun ◽  
XY Liu ◽  
GL Shi ◽  
...  

The visible light–driven ZnS–TiO2/RGO nanocomposites, with good visible light–driven photocatalytic activity, utilizing graphene oxide as precursor, were successfully synthesized via a facile solvothermal thermal method with graphene oxide as precursor. Ti4+ ions were derived from titanium dioxide powder (TiO2, P25) and tetrabutyl titanate. S2− ions and Zn2+ ions were provided by sodium sulfide and zinc acetate, respectively. The photocatalytic and absorbance activity of the nanocomposites was investigated through the photocatalytic degradation of methylene blue in aqueous solution. The results showed that the ZnS–TiO2/RGO nanocomposite prepared by tetrabutyl titanate exhibited better photocatalytic and adsorbance activity for methylene blue under visible light irradiation and its photocatalytic efficiency reached 90% in 60-min light irradiation, almost 1.5 times that of the synthesized ZnS–TiO2/RGO by P25, which was attributed to a cooperative reaction because of an increase of photo-absorption effect of graphene sheets and photocatalytic effect of ZnS nanoparticles. The adsorbance activity for ZnS–TiO2/RGO–tetrabutyl titanate) reached 48%, which was almost 1.7 times higher than that of ZnS–TiO2/RGO-P25 and almost 3.7 times higher than that of ZnS/RGO.


2016 ◽  
Vol 27 (4) ◽  
pp. 511-517 ◽  
Author(s):  
Le-Chen Chen ◽  
Shan Lei ◽  
Mo-Zhen Wang ◽  
Jun Yang ◽  
Xue-Wu Ge

2017 ◽  
Vol 2017 ◽  
pp. 1-10 ◽  
Author(s):  
Jin Wang ◽  
Qiufang Yao ◽  
Chengmin Sheng ◽  
Chunde Jin ◽  
Qingfeng Sun

Via a one-step ultrasonication method, cellulose nanofibril/graphene oxide hybrid (GO-CNF) aerogel was successfully prepared. The as-prepared GO-CNF possessed interconnected 3D network microstructure based on GO nanosheets grown along CNF through hydrogen bonds. The aerogel exhibited superior adsorption capacity toward four kinds of antibiotics. The removal percentages (R%) of these antibiotics were 81.5%, 79.5%, 79.1%, and 73.9% for Doxycycline (DXC), Chlortetracycline (CTC), Oxytetracycline (OTC), and tetracycline (TC), respectively. Simultaneously, the adsorption isotherms were well fitted to Langmuir model and kinetics study implied that the adsorption process was attributed to pseudo-second-order model. The maximum theoretical adsorption capacities of GO-CNF were 469.7, 396.5, 386.5, and 343.8 mg·g−1 for DXC, CTC, OTC, and TC, respectively, calculated by the Langmuir isotherm models. After five cycles, importantly, the regenerated aerogels still could be used with little degradation of adsorption property. Consequently, the as-synthesized GO-CNF was a successful application of effective removal of antibiotics.


The Analyst ◽  
2018 ◽  
Vol 143 (1) ◽  
pp. 208-214 ◽  
Author(s):  
Chang Liu ◽  
Yan-Lei Hu ◽  
Wen-Jing Deng ◽  
Qing-Shan Pan ◽  
Jin-Tao Yi ◽  
...  

Cytochrome c (Cyt c) and caspase-3 are the key mediators in apoptotic signaling.


Sensors ◽  
2014 ◽  
Vol 14 (6) ◽  
pp. 9984-9994 ◽  
Author(s):  
Camille De Souza ◽  
Samia Zrig ◽  
Dengjun Wang ◽  
Minh-Chau Pham ◽  
Benoit Piro

2019 ◽  
Vol 221 ◽  
pp. 47-57 ◽  
Author(s):  
Saisai Ding ◽  
Shiping Sun ◽  
Hongliang Xu ◽  
Biye Yang ◽  
Yingying Liu ◽  
...  

Polymers ◽  
2020 ◽  
Vol 12 (9) ◽  
pp. 2169
Author(s):  
Wei Zhu ◽  
Xueliang Jiang ◽  
Fangjun Liu ◽  
Feng You ◽  
Chu Yao

Graphene based aerogel has become one of the most likely functional adsorption materials that is applicable to purify various contaminated water sources, such as dye wastewater, because of its high porosity, structural stability, large specific surface area, and high adsorption capacity. In this study, chitosan and graphene oxide were first selected as the matrix to prepare the composite hydrogel through the hydrothermal method, which was further frozen and dried to obtain the target aerogel. The microscopic structures and adsorption capacity of the composite aerogel were then characterized by Fourier transform infrared spectroscopy (FT-IR), scanning electron microscopy (SEM), X-ray powder diffraction (XRD) and N2 (nitrogen) physical adsorption and desorption tests. The results show that the specific surface area of the composite aerogel was reached at 297.431 m2/g, which is higher than that of graphene oxide aerogel and chitosan aerogel. The aperture was reduced to about 3 nm. The adsorption rate of the composite aerogel for the methyl orange solution was as high as 97.2% at pH = 1, and the adsorption capacity was 48.6 mg/g. The adsorption process of the composite aerogel satisfies the Langmuir equation and can be described by the second-order adsorption kinetics. In addition, it is worth noting that this composite aerogel can provide a striking adsorption characteristic on methyl orange due to the combining effects from massive amino groups on chitosan and the structural conjugation of graphene oxide.


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