Continuous-flow stereoselective reduction of prochiral ketones in a whole cell bioreactor with natural deep eutectic solvents

2021 ◽  
Author(s):  
Francesca Annunziata ◽  
Alessandra Guaglio ◽  
Paola Conti ◽  
Lucia Tamborini ◽  
Raffaella Gandolfi
Keyword(s):  
Continuous Flow ◽  
Flow Reactor ◽  
Deep Eutectic Solvents ◽  
Packed Bed ◽  
Efficient Production ◽  
Stereoselective Reduction ◽  
Whole Cells ◽  
Natural Deep Eutectic Solvents ◽  
Prochiral Ketones ◽  
Immobilized Whole Cells

Immobilized whole cells of Rhodotorula rubra MIM147 were used in a packed bed flow reactor for the enantioselective reduction of β-ketonitriles and for the efficient production of a key building...

Co‐immobilized Whole Cells with ω‐Transaminase and Ketoreductase Activities for Continuous‐Flow Cascade Reactions

ChemBioChem ◽  
2018 ◽  
Vol 19 (17) ◽  
pp. 1845-1848 ◽  
Author(s):  
László Nagy‐Győr ◽  
Emese Abaházi ◽  
Viktória Bódai ◽  
Péter Sátorhelyi ◽  
Balázs Erdélyi ◽  
...  
Keyword(s):  
Continuous Flow ◽  
Cascade Reactions ◽  
Whole Cells ◽  
Immobilized Whole Cells

Immobilized Whole Cells as Effective Catalysts for Chiral Alcohol Production

2009 ◽  
Vol 62 (9) ◽  
pp. 1034 ◽  
Author(s):  
Jeck Fei Ng ◽  
Stephan Jaenicke
Keyword(s):  
Flow Reactor ◽  
Batch Reactor ◽  
Enantiomeric Excess ◽  
Lactobacillus Brevis ◽  
Packed Bed ◽  
Alginate Beads ◽  
Packed Bed Reactor ◽  
Process Conditions ◽  
Alcohol Production ◽  
Whole Cells

Recombinant Escherichia coli overexpressing the gene LbADH, which encodes for an alcohol dehydrogenase from Lactobacillus brevis, was successfully transformed and cultured. The cells are able to catalyze the reduction of pro-chiral ketones, e.g. ethyl acetoacetate into R-(–)ethyl hydroxybutyrate (EHB) with high conversion and enantiomeric excess >99%. Immobilizing the whole cells in alginate beads leads to a catalyst with improved stability and ease of handling while maintaining the high activity of the free cells. The whole-cell catalyst was tested in a stirred batch reactor (CSTR) and in a continuously operated packed-bed reactor. An Mg2+ concentration of 2 mM was crucial for maintaining the activity of the biocatalyst. After a partial optimization of the process conditions, a productivity of 1.4 gEHB gwcw–1 h–1 could be maintained in a continuous flow reactor over a prolonged period of time.

Enzymatic synthesis of a series of alkyl esters using novozyme 435 in a packed-bed, miniaturized, continuous flow reactor

2008 ◽  
Vol 26 (6) ◽  
pp. 466-472 ◽  
Author(s):  
L. L. Woodcock ◽  
C. Wiles ◽  
G. M. Greenway ◽  
P. Watts ◽  
A. Wells ◽  
...  
Keyword(s):  
Enzymatic Synthesis ◽  
Continuous Flow ◽  
Flow Reactor ◽  
Packed Bed ◽  
Alkyl Esters ◽  
Continuous Flow Reactor ◽  
Novozyme 435

scholarly journals Ene-reductase transformation of massoia lactone to δ-decalactone in a continuous-flow reactor

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Ewa Szczepańska ◽  
Danilo Colombo ◽  
Francesca Tentori ◽  
Teresa Olejniczak ◽  
Elisabetta Brenna ◽  
...  
Keyword(s):  
Continuous Flow ◽  
Membrane Filtration ◽  
Flow Reactor ◽  
Immobilized Cells ◽  
Packed Bed ◽  
Natural Food ◽  
Regeneration System ◽  
Batch Mode ◽  
Cell Lysate ◽  
Continuous Flow Reactor

AbstractThe demand for natural food flavorings increases every year. Biotransformation has become an attractive approach to obtain natural products. In this work, enantiomerically pure (R)-(+)-δ-decalactone was obtained by reduction of the C=C double bond of natural massoia lactone in a continuous-flow reactor. Of 13 different ene-reductases isolated, purified and tested, OYE3 was found to be the most efficient biocatalyst. The selected biocatalyst, either in the form of purified enzyme, cell lysate, whole cells or immobilized cells, was tested in the batch system as well as in the packed-bed flow bioreactor. The biotransformation performed in batch mode, using Ca2+-alginate immobilized cells of Escherichia coli BL21(DE3)/pET30a-OYE3, furnished the desired product with complete conversion in 30 min. The process was intensified using a continuous-flow reactor-membrane filtration system (flow 0.1 mL/min, substrate concentration 10 mM, pH 7, 24 °C) with cell lysate as biocatalyst combined with a cofactor regeneration system, which allowed obtaining > 99% bioconversion of massoia lactone.

scholarly journals Front Cover: Co‐immobilized Whole Cells with ω‐Transaminase and Ketoreductase Activities for Continuous‐Flow Cascade Reactions (ChemBioChem 17/2018)

ChemBioChem ◽  
2018 ◽  
Vol 19 (17) ◽  
pp. 1792-1792
Author(s):  
László Nagy‐Győr ◽  
Emese Abaházi ◽  
Viktória Bódai ◽  
Péter Sátorhelyi ◽  
Balázs Erdélyi ◽  
...  
Keyword(s):  
Continuous Flow ◽  
Cascade Reactions ◽  
Front Cover ◽  
Whole Cells ◽  
Immobilized Whole Cells
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