scholarly journals Ene-reductase transformation of massoia lactone to δ-decalactone in a continuous-flow reactor

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Ewa Szczepańska ◽  
Danilo Colombo ◽  
Francesca Tentori ◽  
Teresa Olejniczak ◽  
Elisabetta Brenna ◽  
...  
Keyword(s):  
Continuous Flow ◽  
Membrane Filtration ◽  
Flow Reactor ◽  
Immobilized Cells ◽  
Packed Bed ◽  
Natural Food ◽  
Regeneration System ◽  
Batch Mode ◽  
Cell Lysate ◽  
Continuous Flow Reactor

AbstractThe demand for natural food flavorings increases every year. Biotransformation has become an attractive approach to obtain natural products. In this work, enantiomerically pure (R)-(+)-δ-decalactone was obtained by reduction of the C=C double bond of natural massoia lactone in a continuous-flow reactor. Of 13 different ene-reductases isolated, purified and tested, OYE3 was found to be the most efficient biocatalyst. The selected biocatalyst, either in the form of purified enzyme, cell lysate, whole cells or immobilized cells, was tested in the batch system as well as in the packed-bed flow bioreactor. The biotransformation performed in batch mode, using Ca2+-alginate immobilized cells of Escherichia coli BL21(DE3)/pET30a-OYE3, furnished the desired product with complete conversion in 30 min. The process was intensified using a continuous-flow reactor-membrane filtration system (flow 0.1 mL/min, substrate concentration 10 mM, pH 7, 24 °C) with cell lysate as biocatalyst combined with a cofactor regeneration system, which allowed obtaining > 99% bioconversion of massoia lactone.

Enzymatic synthesis of a series of alkyl esters using novozyme 435 in a packed-bed, miniaturized, continuous flow reactor

2008 ◽  
Vol 26 (6) ◽  
pp. 466-472 ◽  
Author(s):  
L. L. Woodcock ◽  
C. Wiles ◽  
G. M. Greenway ◽  
P. Watts ◽  
A. Wells ◽  
...  
Keyword(s):  
Enzymatic Synthesis ◽  
Continuous Flow ◽  
Flow Reactor ◽  
Packed Bed ◽  
Alkyl Esters ◽  
Continuous Flow Reactor ◽  
Novozyme 435

Renewable Energy from Agricultural Waste

2018 ◽  
Vol 69 (6) ◽  
pp. 1363-1366 ◽  
Author(s):  
Stefania Daniela Bran ◽  
Petre Chipurici ◽  
Mariana Bran ◽  
Alexandru Vlaicu
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Gas Chromatography ◽  
Continuous Flow ◽  
Laboratory Experiments ◽  
Fermentation Process ◽  
Flow Reactor ◽  
Agricultural Waste ◽  
Continuous Flow Reactor ◽  
Natural Support ◽  
Co2 Flow

This paper has aimed at evaluating the concentration of bioethanol obtained using sunflower stem as natural support, molasses as carbon source and Saccharomyces cerevisiae yeast in a continuous flow reactor. The natural support was tested to investigate the immobilization/growth of S. cerevisiae yeast. The concentration of bioethanol produced by fermentation was analyzed by gas chromatography using two methods: aqueous solutions and extraction in organic phase. The CO2 flow obtained during the fermentation process was considered to estimate when the yeast was deactivated. The laboratory experiments have highlighted that the use of plant-based wastes to bioconversion in ethanol could be a non-pollutant and sustainable alternative.

Design of a continuous flow immobilized-cell reactor for biosynthetical production of L-aspartic acid

1985 ◽  
Vol 50 (10) ◽  
pp. 2122-2133 ◽  
Author(s):  
Jindřich Zahradník ◽  
Marie Fialová ◽  
Jan Škoda ◽  
Helena Škodová
Keyword(s):  
Aspartic Acid ◽  
Continuous Flow ◽  
Immobilized Cells ◽  
Scale Up ◽  
Fixed Bed ◽  
Packed Bed ◽  
Fixed Bed Reactor ◽  
Experimental Program ◽  
Design Parameters ◽  
Immobilized Cell

An experimental study was carried out aimed at establishing a data base for an optimum design of a continuous flow fixed-bed reactor for biotransformation of ammonium fumarate to L-aspartic acid catalyzed by immobilized cells of the strain Escherichia alcalescens dispar group. The experimental program included studies of the effect of reactor geometry, catalytic particle size, and packed bed arrangement on reactor hydrodynamics and on the rate of substrate conversion. An expression for the effective reaction rate was derived including the effect of mass transfer and conditions of the safe conversion-data scale-up were defined. Suggestions for the design of a pilot plant reactor (100 t/year) were formulated and decisive design parameters of such reactor were estimated for several variants of problem formulation.

Thermocatalytic Hydrodeoxygenation and Depolymerization of Waste Lignin to Oxygenates and Biofuels in a Continuous Flow Reactor at Atmospheric Pressure

2020 ◽  
Vol 8 (35) ◽  
pp. 13195-13205 ◽  
Author(s):  
Swathi Mukundan ◽  
Daria Boffito ◽  
Abhijit Shrotri ◽  
Luqman Atanda ◽  
Jorge Beltramini ◽  
...  
Keyword(s):  
Atmospheric Pressure ◽  
Continuous Flow ◽  
Flow Reactor ◽  
Continuous Flow Reactor

scholarly journals Development of a tubular continuous flow reactor for the investigation of improved gas–solid interaction in photocatalytic CO2 reduction on TiO2

2019 ◽  
Vol 18 (2) ◽  
pp. 314-318 ◽  
Author(s):  
Martin Dilla ◽  
Ahmet E. Becerikli ◽  
Alina Jakubowski ◽  
Robert Schlögl ◽  
Simon Ristig
Keyword(s):  
Gas Phase ◽  
Continuous Flow ◽  
Flow Reactor ◽  
Tubular Reactor ◽  
Co2 Reduction ◽  
Continuous Flow Reactor ◽  
Reactor Geometry

Newly developed tubular reactor geometry allows intensive gas–solid interaction in photocatalytic gas-phase CO2 reduction.

Design of a novel continuous flow reactor for low pH viral inactivation

2017 ◽  
Vol 115 (3) ◽  
pp. 606-616 ◽  
Author(s):  
Stephanie A. Parker ◽  
Linus Amarikwa ◽  
Kevin Vehar ◽  
Raquel Orozco ◽  
Scott Godfrey ◽  
...  
Keyword(s):  
Continuous Flow ◽  
Flow Reactor ◽  
Low Ph ◽  
Viral Inactivation ◽  
Continuous Flow Reactor

Stability of the first-generation Grubbs metathesis catalyst in a continuous flow reactor

2006 ◽  
Vol 691 (24-25) ◽  
pp. 5197-5203 ◽  
Author(s):  
Zenon Lysenko ◽  
Bob R. Maughon ◽  
Tezi Mokhtar-Zadeh ◽  
Michael L. Tulchinsky
Keyword(s):  
Continuous Flow ◽  
First Generation ◽  
Flow Reactor ◽  
Continuous Flow Reactor ◽  
Metathesis Catalyst
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